Restriction map for
pF1KB9728
(Linear) (Six-base) MAPSORT of: KIBB9728.seq Check: 3391 from: 1 to: 1332
KIBB9728.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
Acc65I G'GTAC_C
Cuts at: 0 948 1332
Size: 948 384
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 570 1332
Size: 570 762
AflIII A'CryG_T
Cuts at: 0 824 1332
Size: 824 508
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1073 1332
Size: 1073 259
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 547 579 1332
Size: 547 32 753
Fragments arranged by size:
753 547 32
AlwNI CAG_nnn'CTG
Cuts at: 0 387 1332
Size: 387 945
AvaI C'yCGr_G
Cuts at: 0 276 292 493 837 880 1332
Size: 276 16 201 344 43 452
Fragments arranged by size:
452 344 276 201 43 16
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 940 973 1332
Size: 940 33 359
Fragments arranged by size:
940 359 33
BanI G'GyrC_C
Cuts at: 0 244 259 415 684 696 772 867 948
Size: 244 15 156 269 12 76 95 81
Cuts at: 948 1030 1332
Size: 82 302
Fragments arranged by size:
302 269 244 156 95 82 81 76
15 12
BanII G_rGCy'C
Cuts at: 0 88 277 346 562 1006 1024 1332
Size: 88 189 69 216 444 18 308
Fragments arranged by size:
444 308 216 189 88 69 18
BbeI G_GCGC'C
Cuts at: 0 248 263 419 688 700 776 1034 1332
Size: 248 15 156 269 12 76 258 298
Fragments arranged by size:
298 269 258 248 156 76 15 12
BbvCI CC'TCA_GC
Cuts at: 0 1263 1332
Size: 1263 69
BglI GCCn_nnn'nGGC
Cuts at: 0 208 268 722 1090 1332
Size: 208 60 454 368 242
Fragments arranged by size:
454 368 242 208 60
BglII A'GATC_T
Cuts at: 0 374 1332
Size: 374 958
BlpI GC'TnA_GC
Cuts at: 0 367 1332
Size: 367 965
Bme1580I G_kGCm'C
Cuts at: 0 872 1332
Size: 872 460
BmgBI CAC'GTC
Cuts at: 0 816 1332
Size: 816 516
BmtI G_CTAG'C
Cuts at: 0 1271 1332
Size: 1271 61
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 150 576 1182 1332
Size: 150 426 606 150
Fragments arranged by size:
606 426 150 150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 1038 1089 1147 1187 1332
Size: 52 986 51 58 40 145
Fragments arranged by size:
986 145 58 52 51 40
Bpu10I CC'TnA_GC
Cuts at: 0 1263 1332
Size: 1263 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 1030 1332
Size: 592 438 302
Fragments arranged by size:
592 438 302
BsaI GGTCTCn'nnnn_
Cuts at: 0 121 1332
Size: 121 1211
BsaHI Gr'CG_yC
Cuts at: 0 245 260 416 652 685 697 761 773
Size: 245 15 156 236 33 12 64 12
Cuts at: 773 887 1031 1066 1332
Size: 114 144 35 266
Fragments arranged by size:
266 245 236 156 144 114 64 35
33 15 12 12
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 547 577 1332
Size: 547 30 755
Fragments arranged by size:
755 547 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 126 129 132 174 568 1332
Size: 126 3 3 42 394 764
Fragments arranged by size:
764 394 126 42 3 3
BseYI C'CCAG_C
Cuts at: 0 349 778 1069 1332
Size: 349 429 291 263
Fragments arranged by size:
429 349 291 263
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 1084 1332
Size: 66 1018 248
Fragments arranged by size:
1018 248 66
BsiEI CG_ry'CG
Cuts at: 0 577 899 1332
Size: 577 322 433
Fragments arranged by size:
577 433 322
BsiHKAI G_wGCw'C
Cuts at: 0 277 346 1024 1332
Size: 277 69 678 308
Fragments arranged by size:
678 308 277 69
BsmI GAATG_Cn'
Cuts at: 0 988 1332
Size: 988 344
Bsp1286I G_dGCh'C
Cuts at: 0 88 277 346 562 872 1006 1024 1332
Size: 88 189 69 216 310 134 18 308
Fragments arranged by size:
310 308 216 189 134 88 69 18
BsrBI CCG'CTC
Cuts at: 0 33 254 635 1332
Size: 33 221 381 697
Fragments arranged by size:
697 381 221 33
BsrFI r'CCGG_y
Cuts at: 0 24 572 763 1332
Size: 24 548 191 569
Fragments arranged by size:
569 548 191 24
BssHII G'CGCG_C
Cuts at: 0 62 394 1332
Size: 62 332 938
Fragments arranged by size:
938 332 62
BssSI C'ACGA_G
Cuts at: 0 999 1332
Size: 999 333
BstXI CCAn_nnnn'nTGG
Cuts at: 0 852 1332
Size: 852 480
BstYI r'GATC_y
Cuts at: 0 374 1332
Size: 374 958
Bsu36I CC'TnA_GG
Cuts at: 0 485 1332
Size: 485 847
BtgI C'CryG_G
Cuts at: 0 411 749 872 965 1332
Size: 411 338 123 93 367
Fragments arranged by size:
411 367 338 123 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 300 1332
Size: 300 1032
DraIII CAC_nnn'GTG
Cuts at: 0 627 1332
Size: 627 705
EaeI y'GGCC_r
Cuts at: 0 194 332 533 574 854 896 962 1332
Size: 194 138 201 41 280 42 66 370
Fragments arranged by size:
370 280 201 194 138 66 42 41
EagI C'GGCC_G
Cuts at: 0 574 896 1332
Size: 574 322 436
Fragments arranged by size:
574 436 322
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 25 147 556 606 1332
Size: 25 122 409 50 726
Fragments arranged by size:
726 409 122 50 25
EcoICRI GAG'CTC
Cuts at: 0 275 344 1022 1332
Size: 275 69 678 310
Fragments arranged by size:
678 310 275 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 570 1038 1089 1147 1187 1332
Size: 52 518 468 51 58 40 145
Fragments arranged by size:
518 468 145 58 52 51 40
EcoO109I rG'GnC_Cy
Cuts at: 0 489 877 1161 1332
Size: 489 388 284 171
Fragments arranged by size:
489 388 284 171
HaeII r_GCGC'y
Cuts at: 0 203 248 263 360 419 607 688 700
Size: 203 45 15 97 59 188 81 12
Cuts at: 700 776 977 1034 1332
Size: 76 201 57 298
Fragments arranged by size:
298 203 201 188 97 81 76 59
57 45 15 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1290 1322 1332
Size: 1290 32 10
Fragments arranged by size:
1290 32 10
KasI G'GCGC_C
Cuts at: 0 244 259 415 684 696 772 1030 1332
Size: 244 15 156 269 12 76 258 302
Fragments arranged by size:
302 269 258 244 156 76 15 12
KpnI G_GTAC'C
Cuts at: 0 952 1332
Size: 952 380
MscI TGG'CCA
Cuts at: 0 334 856 1332
Size: 334 522 476
Fragments arranged by size:
522 476 334
MslI CAynn'nnrTG
Cuts at: 0 328 850 1332
Size: 328 522 482
Fragments arranged by size:
522 482 328
MspA1I CmG'CkG
Cuts at: 0 413 751 860 874 1332
Size: 413 338 109 14 458
Fragments arranged by size:
458 413 338 109 14
NaeI GCC'GGC
Cuts at: 0 26 574 765 1332
Size: 26 548 191 567
Fragments arranged by size:
567 548 191 26
NarI GG'CG_CC
Cuts at: 0 245 260 416 685 697 773 1031 1332
Size: 245 15 156 269 12 76 258 301
Fragments arranged by size:
301 269 258 245 156 76 15 12
NcoI C'CATG_G
Cuts at: 0 965 1332
Size: 965 367
NgoMIV G'CCGG_C
Cuts at: 0 24 572 763 1332
Size: 24 548 191 569
Fragments arranged by size:
569 548 191 24
NheI G'CTAG_C
Cuts at: 0 1267 1332
Size: 1267 65
NruI TCG'CGA
Cuts at: 0 1157 1332
Size: 1157 175
NspI r_CATG'y
Cuts at: 0 828 1113 1332
Size: 828 285 219
Fragments arranged by size:
828 285 219
PasI CC'CwG_GG
Cuts at: 0 666 1332
Size: 666 666
PciI A'CATG_T
Cuts at: 0 824 1332
Size: 824 508
PflMI CCAn_nnn'nTGG
Cuts at: 0 627 1332
Size: 627 705
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 532 547 564 579 1332
Size: 532 15 17 15 753
Fragments arranged by size:
753 532 17 15 15
PpuMI rG'GwC_Cy
Cuts at: 0 877 1161 1332
Size: 877 284 171
Fragments arranged by size:
877 284 171
PstI C_TGCA'G
Cuts at: 0 392 1332
Size: 392 940
PvuII CAG'CTG
Cuts at: 0 860 1332
Size: 860 472
SacI G_AGCT'C
Cuts at: 0 277 346 1024 1332
Size: 277 69 678 308
Fragments arranged by size:
678 308 277 69
SacII CC_GC'GG
Cuts at: 0 414 752 875 1332
Size: 414 338 123 457
Fragments arranged by size:
457 414 338 123
SanDI GG'GwC_CC
Cuts at: 0 877 1332
Size: 877 455
SbfI CC_TGCA'GG
Cuts at: 0 392 1332
Size: 392 940
SfcI C'TryA_G
Cuts at: 0 388 1332
Size: 388 944
SfoI GGC'GCC
Cuts at: 0 246 261 417 686 698 774 1032 1332
Size: 246 15 156 269 12 76 258 300
Fragments arranged by size:
300 269 258 246 156 76 15 12
SgrAI Cr'CCGG_yG
Cuts at: 0 763 1332
Size: 763 569
SmaI CCC'GGG
Cuts at: 0 839 882 1332
Size: 839 43 450
Fragments arranged by size:
839 450 43
SmlI C'TyrA_G
Cuts at: 0 607 1045 1332
Size: 607 438 287
Fragments arranged by size:
607 438 287
StuI AGG'CCT
Cuts at: 0 1097 1332
Size: 1097 235
StyI C'CwwG_G
Cuts at: 0 965 1332
Size: 965 367
XmaI C'CCGG_G
Cuts at: 0 837 880 1332
Size: 837 43 452
Fragments arranged by size:
837 452 43
Enzymes that do cut:
Acc65I AcuI AflIII AlfI AloI AlwNI AvaI BaeI
BanI BanII BbeI BbvCI BglI BglII BlpI Bme1580I
BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BsrBI BsrFI BssHII BssSI BstXI BstYI Bsu36I BtgI
BtgZI DraIII EaeI EagI EciI EcoICRI Eco57MI EcoO109I
HaeII Hin4I KasI KpnI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NruI NspI PasI PciI
PflMI PpiI PpuMI PstI PvuII SacI SacII SanDI
SbfI SfcI SfoI SgrAI SmaI SmlI StuI StyI
XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BamHI BbsI BcgI BciVI BclI BfrBI BmrI BsaAI
BsaBI BsaWI BsiWI BsmBI BspEI BspHI BspMI BsrDI
BsrGI BstAPI BstBI BstEII BstZ17I BtsI ClaI CspCI
DraI DrdI EarI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI MfeI MluI MmeI
NdeI NotI NsiI PacI PfoI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SalI SapI
ScaI SexAI SfiI SgfI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmnI ZraI