Restriction map for
pF1KB9727
(Linear) (Six-base) MAPSORT of: KIBB9727.seq Check: 9710 from: 1 to: 1317
KIBB9727.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 682 1317
Size: 682 635
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 896 1317
Size: 44 852 421
Fragments arranged by size:
852 421 44
BanI G'GyrC_C
Cuts at: 0 698 1317
Size: 698 619
BanII G_rGCy'C
Cuts at: 0 557 1317
Size: 557 760
BbsI GAAGACnn'nnnn_
Cuts at: 0 746 1317
Size: 746 571
BclI T'GATC_A
Cuts at: 0 110 1317
Size: 110 1207
BfrBI ATG'CAT
Cuts at: 0 565 1317
Size: 565 752
BglII A'GATC_T
Cuts at: 0 786 1317
Size: 786 531
Bme1580I G_kGCm'C
Cuts at: 0 701 1317
Size: 701 616
BmtI G_CTAG'C
Cuts at: 0 686 1317
Size: 686 631
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 991 1317
Size: 991 326
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 695 1016 1317
Size: 166 529 321 301
Fragments arranged by size:
529 321 301 166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 631 1317
Size: 139 492 686
Fragments arranged by size:
686 492 139
BsaI GGTCTCn'nnnn_
Cuts at: 0 337 1063 1317
Size: 337 726 254
Fragments arranged by size:
726 337 254
BsaBI GATnn'nnATC
Cuts at: 0 791 1317
Size: 791 526
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 1317
Size: 148 1169
BseYI C'CCAG_C
Cuts at: 0 633 937 1317
Size: 633 304 380
Fragments arranged by size:
633 380 304
BsiHKAI G_wGCw'C
Cuts at: 0 972 1317
Size: 972 345
Bsp1286I G_dGCh'C
Cuts at: 0 557 701 972 1317
Size: 557 144 271 345
Fragments arranged by size:
557 345 271 144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 682 1317
Size: 682 635
BsrDI GCAATG_nn'
Cuts at: 0 506 565 1289 1317
Size: 506 59 724 28
Fragments arranged by size:
724 506 59 28
BssSI C'ACGA_G
Cuts at: 0 982 1317
Size: 982 335
BstAPI GCAn_nnn'nTGC
Cuts at: 0 670 1317
Size: 670 647
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1188 1317
Size: 1188 129
BstYI r'GATC_y
Cuts at: 0 786 1317
Size: 786 531
BtgI C'CryG_G
Cuts at: 0 1004 1317
Size: 1004 313
BtsI GCAGTG_nn'
Cuts at: 0 970 1059 1317
Size: 970 89 258
Fragments arranged by size:
970 258 89
DraI TTT'AAA
Cuts at: 0 197 1317
Size: 197 1120
DrdI GACnn_nn'nnGTC
Cuts at: 0 13 1077 1183 1317
Size: 13 1064 106 134
Fragments arranged by size:
1064 134 106 13
EaeI y'GGCC_r
Cuts at: 0 1197 1231 1317
Size: 1197 34 86
Fragments arranged by size:
1197 86 34
EarI CTCTTCn'nnn_
Cuts at: 0 479 950 1127 1136 1317
Size: 479 471 177 9 181
Fragments arranged by size:
479 471 181 177 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 166 695 896 1016 1317
Size: 44 122 529 201 120 301
Fragments arranged by size:
529 301 201 122 120 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 443 1317
Size: 443 874
EcoO109I rG'GnC_Cy
Cuts at: 0 727 1305 1317
Size: 727 578 12
Fragments arranged by size:
727 578 12
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 805 1317
Size: 805 512
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 478 510 1317
Size: 478 32 807
Fragments arranged by size:
807 478 32
MslI CAynn'nnrTG
Cuts at: 0 769 901 1186 1317
Size: 769 132 285 131
Fragments arranged by size:
769 285 132 131
MspA1I CmG'CkG
Cuts at: 0 458 1027 1042 1111 1317
Size: 458 569 15 69 206
Fragments arranged by size:
569 458 206 69 15
NcoI C'CATG_G
Cuts at: 0 1004 1317
Size: 1004 313
NdeI CA'TA_TG
Cuts at: 0 867 1317
Size: 867 450
NheI G'CTAG_C
Cuts at: 0 682 1317
Size: 682 635
NsiI A_TGCA'T
Cuts at: 0 567 1317
Size: 567 750
NspI r_CATG'y
Cuts at: 0 91 1299 1317
Size: 91 1208 18
Fragments arranged by size:
1208 91 18
PpuMI rG'GwC_Cy
Cuts at: 0 727 1305 1317
Size: 727 578 12
Fragments arranged by size:
727 578 12
PstI C_TGCA'G
Cuts at: 0 246 1317
Size: 246 1071
PvuII CAG'CTG
Cuts at: 0 1027 1111 1317
Size: 1027 84 206
Fragments arranged by size:
1027 206 84
ScaI AGT'ACT
Cuts at: 0 402 1317
Size: 402 915
SfcI C'TryA_G
Cuts at: 0 242 393 1212 1317
Size: 242 151 819 105
Fragments arranged by size:
819 242 151 105
SmlI C'TyrA_G
Cuts at: 0 154 610 1317
Size: 154 456 707
Fragments arranged by size:
707 456 154
SphI G_CATG'C
Cuts at: 0 91 1317
Size: 91 1226
StuI AGG'CCT
Cuts at: 0 831 1317
Size: 831 486
StyI C'CwwG_G
Cuts at: 0 92 98 858 1004 1317
Size: 92 6 760 146 313
Fragments arranged by size:
760 313 146 92 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 361 1317
Size: 361 956
TatI w'GTAC_w
Cuts at: 0 400 460 1317
Size: 400 60 857
Fragments arranged by size:
857 400 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 12 44 108 140 711 743 1317
Size: 12 32 64 32 571 32 574
Fragments arranged by size:
574 571 64 32 32 32 12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 865 1188 1317
Size: 865 323 129
Fragments arranged by size:
865 323 129
XmnI GAAnn'nnTTC
Cuts at: 0 788 1317
Size: 788 529
Enzymes that do cut:
AarI AcuI BanI BanII BbsI BclI BfrBI BglII
Bme1580I BmtI BplI BpmI BpuEI BsaI BsaBI BseRI
BseYI BsiHKAI Bsp1286I BspMI BsrDI BssSI BstAPI BstXI
BstYI BtgI BtsI DraI DrdI EaeI EarI Eco57MI
EcoNI EcoO109I FalI Hin4I MslI MspA1I NcoI NdeI
NheI NsiI NspI PpuMI PstI PvuII ScaI SfcI
SmlI SphI StuI StyI TaqII TatI TstI XcmI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI
BbvCI BcgI BciVI BglI BlpI BmgBI BmrI Bpu10I
BsaAI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII EagI
EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NgoMIV NotI NruI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI
XhoI XmaI ZraI