Restriction map for
pF1KB9714
(Linear) (Six-base) MAPSORT of: KIBB9714.seq Check: 4912 from: 1 to: 1146
KIBB9714.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 223 1146
Size: 223 923
AccI GT'mk_AC
Cuts at: 0 341 917 1146
Size: 341 576 229
Fragments arranged by size:
576 341 229
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 430 1146
Size: 370 60 716
Fragments arranged by size:
716 370 60
AlwNI CAG_nnn'CTG
Cuts at: 0 161 807 897 1072 1146
Size: 161 646 90 175 74
Fragments arranged by size:
646 175 161 90 74
ApaI G_GGCC'C
Cuts at: 0 330 1146
Size: 330 816
ApoI r'AATT_y
Cuts at: 0 1128 1146
Size: 1128 18
BanI G'GyrC_C
Cuts at: 0 988 1146
Size: 988 158
BanII G_rGCy'C
Cuts at: 0 91 203 330 478 814 998 1146
Size: 91 112 127 148 336 184 148
Fragments arranged by size:
336 184 148 148 127 112 91
BbvCI CC'TCA_GC
Cuts at: 0 557 1146
Size: 557 589
BciVI GTATCCnnnnn_n'
Cuts at: 0 657 1146
Size: 657 489
BglI GCCn_nnn'nGGC
Cuts at: 0 538 1146
Size: 538 608
BlpI GC'TnA_GC
Cuts at: 0 463 808 1146
Size: 463 345 338
Fragments arranged by size:
463 345 338
Bme1580I G_kGCm'C
Cuts at: 0 330 1146
Size: 330 816
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 695 833 1146
Size: 695 138 313
Fragments arranged by size:
695 313 138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 986 1146
Size: 226 760 160
Fragments arranged by size:
760 226 160
Bpu10I CC'TnA_GC
Cuts at: 0 349 557 1146
Size: 349 208 589
Fragments arranged by size:
589 349 208
BsaI GGTCTCn'nnnn_
Cuts at: 0 202 949 962 1146
Size: 202 747 13 184
Fragments arranged by size:
747 202 184 13
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 878 908 1012 1042 1146
Size: 878 30 104 30 104
Fragments arranged by size:
878 104 104 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 54 1146
Size: 37 17 1092
Fragments arranged by size:
1092 37 17
BseYI C'CCAG_C
Cuts at: 0 72 477 841 1072 1146
Size: 72 405 364 231 74
Fragments arranged by size:
405 364 231 74 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 1146
Size: 199 947
BsiEI CG_ry'CG
Cuts at: 0 44 1146
Size: 44 1102
BsiHKAI G_wGCw'C
Cuts at: 0 814 1146
Size: 814 332
BsmI GAATG_Cn'
Cuts at: 0 625 1146
Size: 625 521
Bsp1286I G_dGCh'C
Cuts at: 0 91 203 330 478 814 998 1146
Size: 91 112 127 148 336 184 148
Fragments arranged by size:
336 184 148 148 127 112 91
BspHI T'CATG_A
Cuts at: 0 857 1146
Size: 857 289
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 223 1146
Size: 223 923
BsrBI CCG'CTC
Cuts at: 0 82 453 1146
Size: 82 371 693
Fragments arranged by size:
693 371 82
BstAPI GCAn_nnn'nTGC
Cuts at: 0 177 1146
Size: 177 969
BstXI CCAn_nnnn'nTGG
Cuts at: 0 964 1146
Size: 964 182
BstYI r'GATC_y
Cuts at: 0 927 1146
Size: 927 219
BstZ17I GTA'TAC
Cuts at: 0 342 1146
Size: 342 804
BtgI C'CryG_G
Cuts at: 0 534 1146
Size: 534 612
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 285 1146
Size: 285 861
BtsI GCAGTG_nn'
Cuts at: 0 178 187 1146
Size: 178 9 959
Fragments arranged by size:
959 178 9
EaeI y'GGCC_r
Cuts at: 0 758 899 1146
Size: 758 141 247
Fragments arranged by size:
758 247 141
EarI CTCTTCn'nnn_
Cuts at: 0 328 1146
Size: 328 818
EcoICRI GAG'CTC
Cuts at: 0 812 1146
Size: 812 334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 370 430 986 1146
Size: 226 144 60 556 160
Fragments arranged by size:
556 226 160 144 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 412 1146
Size: 412 734
EcoRI G'AATT_C
Cuts at: 0 1128 1146
Size: 1128 18
HaeII r_GCGC'y
Cuts at: 0 582 1146
Size: 582 564
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 929 961 1012 1044 1146
Size: 929 32 51 32 102
Fragments arranged by size:
929 102 51 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 960 1146
Size: 960 186
MscI TGG'CCA
Cuts at: 0 760 901 1146
Size: 760 141 245
Fragments arranged by size:
760 245 141
MslI CAynn'nnrTG
Cuts at: 0 983 1146
Size: 983 163
MspA1I CmG'CkG
Cuts at: 0 117 423 1146
Size: 117 306 723
Fragments arranged by size:
723 306 117
NcoI C'CATG_G
Cuts at: 0 534 1146
Size: 534 612
NspI r_CATG'y
Cuts at: 0 978 1146
Size: 978 168
PflMI CCAn_nnn'nTGG
Cuts at: 0 1011 1146
Size: 1011 135
PspOMI G'GGCC_C
Cuts at: 0 326 1146
Size: 326 820
PstI C_TGCA'G
Cuts at: 0 664 690 1085 1146
Size: 664 26 395 61
Fragments arranged by size:
664 395 61 26
PvuI CG_AT'CG
Cuts at: 0 44 1146
Size: 44 1102
PvuII CAG'CTG
Cuts at: 0 423 1146
Size: 423 723
SacI G_AGCT'C
Cuts at: 0 814 1146
Size: 814 332
SbfI CC_TGCA'GG
Cuts at: 0 664 1146
Size: 664 482
SfcI C'TryA_G
Cuts at: 0 660 686 918 1081 1146
Size: 660 26 232 163 65
Fragments arranged by size:
660 232 163 65 26
StuI AGG'CCT
Cuts at: 0 634 1146
Size: 634 512
StyI C'CwwG_G
Cuts at: 0 534 1146
Size: 534 612
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 814 1033 1146
Size: 814 219 113
Fragments arranged by size:
814 219 113
TatI w'GTAC_w
Cuts at: 0 239 1115 1146
Size: 239 876 31
Fragments arranged by size:
876 239 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 878 910 1146
Size: 878 32 236
Fragments arranged by size:
878 236 32
Tth111I GACn'n_nGTC
Cuts at: 0 941 1146
Size: 941 205
Enzymes that do cut:
AarI AccI AcuI AlwNI ApaI ApoI BanI BanII
BbvCI BciVI BglI BlpI Bme1580I BplI BpmI Bpu10I
BsaI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BstAPI BstXI BstYI BstZ17I
BtgI BtgZI BtsI EaeI EarI EcoICRI Eco57MI EcoNI
EcoRI HaeII Hin4I MmeI MscI MslI MspA1I NcoI
NspI PflMI PspOMI PstI PvuI PvuII SacI SbfI
SfcI StuI StyI TaqII TatI TstI Tth111I
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BcgI BclI BfrBI
BglII BmgBI BmrI BmtI BpuEI BsaAI BsaBI BsaHI
BsaWI BsiWI BsmBI BspEI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstEII Bsu36I ClaI CspCI DraI DraIII
DrdI EagI EciI EcoO109I EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI RsrII SacII SalI SanDI
SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI XbaI
XcmI XhoI XmaI XmnI ZraI