Restriction map for
pF1KB9710
(Linear) (Six-base) MAPSORT of: KIBB9710.seq Check: 6063 from: 1 to: 1044
KIBB9710.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 858 1044
Size: 858 186
AccI GT'mk_AC
Cuts at: 0 119 727 1044
Size: 119 608 317
Fragments arranged by size:
608 317 119
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 1044
Size: 48 996
AfeI AGC'GCT
Cuts at: 0 218 829 1044
Size: 218 611 215
Fragments arranged by size:
611 218 215
AlwNI CAG_nnn'CTG
Cuts at: 0 393 777 1044
Size: 393 384 267
Fragments arranged by size:
393 384 267
AvaI C'yCGr_G
Cuts at: 0 669 1044
Size: 669 375
BanI G'GyrC_C
Cuts at: 0 706 1044
Size: 706 338
BanII G_rGCy'C
Cuts at: 0 478 950 1044
Size: 478 472 94
Fragments arranged by size:
478 472 94
BbeI G_GCGC'C
Cuts at: 0 710 1044
Size: 710 334
BbsI GAAGACnn'nnnn_
Cuts at: 0 572 671 1044
Size: 572 99 373
Fragments arranged by size:
572 373 99
BglI GCCn_nnn'nGGC
Cuts at: 0 150 1044
Size: 150 894
BmgBI CAC'GTC
Cuts at: 0 725 990 1044
Size: 725 265 54
Fragments arranged by size:
725 265 54
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 612 1044
Size: 612 432
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 622 703 799 1044
Size: 258 364 81 96 245
Fragments arranged by size:
364 258 245 96 81
BsaI GGTCTCn'nnnn_
Cuts at: 0 682 1044
Size: 682 362
BsaBI GATnn'nnATC
Cuts at: 0 632 1044
Size: 632 412
BsaHI Gr'CG_yC
Cuts at: 0 707 1044
Size: 707 337
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 601 700 1044
Size: 601 99 344
Fragments arranged by size:
601 344 99
BseYI C'CCAG_C
Cuts at: 0 45 100 250 347 1044
Size: 45 55 150 97 697
Fragments arranged by size:
697 150 97 55 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 886 1044
Size: 529 357 158
Fragments arranged by size:
529 357 158
BsiEI CG_ry'CG
Cuts at: 0 154 500 1044
Size: 154 346 544
Fragments arranged by size:
544 346 154
BsmBI CGTCTCn'nnnn_
Cuts at: 0 496 1044
Size: 496 548
Bsp1286I G_dGCh'C
Cuts at: 0 478 950 1044
Size: 478 472 94
Fragments arranged by size:
478 472 94
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 858 986 1044
Size: 858 128 58
Fragments arranged by size:
858 128 58
BsrDI GCAATG_nn'
Cuts at: 0 930 1044
Size: 930 114
BsrFI r'CCGG_y
Cuts at: 0 124 149 153 740 1044
Size: 124 25 4 587 304
Fragments arranged by size:
587 304 124 25 4
BstAPI GCAn_nnn'nTGC
Cuts at: 0 393 925 1044
Size: 393 532 119
Fragments arranged by size:
532 393 119
BstYI r'GATC_y
Cuts at: 0 245 1044
Size: 245 799
BtgI C'CryG_G
Cuts at: 0 494 710 1044
Size: 494 216 334
Fragments arranged by size:
494 334 216
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 623 1044
Size: 623 421
BtsI GCAGTG_nn'
Cuts at: 0 798 1044
Size: 798 246
EaeI y'GGCC_r
Cuts at: 0 108 151 314 464 497 642 1044
Size: 108 43 163 150 33 145 402
Fragments arranged by size:
402 163 150 145 108 43 33
EagI C'GGCC_G
Cuts at: 0 151 497 1044
Size: 151 346 547
Fragments arranged by size:
547 346 151
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 436 765 1044
Size: 436 329 279
Fragments arranged by size:
436 329 279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 258 622 703 799 1044
Size: 48 210 364 81 96 245
Fragments arranged by size:
364 245 210 96 81 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 283 1044
Size: 283 761
HaeII r_GCGC'y
Cuts at: 0 42 220 298 430 710 831 1044
Size: 42 178 78 132 280 121 213
Fragments arranged by size:
280 213 178 132 121 78 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 607 639 1044
Size: 607 32 405
Fragments arranged by size:
607 405 32
KasI G'GCGC_C
Cuts at: 0 706 1044
Size: 706 338
MscI TGG'CCA
Cuts at: 0 316 466 644 1044
Size: 316 150 178 400
Fragments arranged by size:
400 316 178 150
MslI CAynn'nnrTG
Cuts at: 0 139 1044
Size: 139 905
MspA1I CmG'CkG
Cuts at: 0 45 367 393 496 704 712 774 1044
Size: 45 322 26 103 208 8 62 270
Fragments arranged by size:
322 270 208 103 62 45 26 8
NarI GG'CG_CC
Cuts at: 0 707 1044
Size: 707 337
PflMI CCAn_nnn'nTGG
Cuts at: 0 681 1044
Size: 681 363
PfoI T'CCnGG_A
Cuts at: 0 952 1044
Size: 952 92
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 778 810 1044
Size: 778 32 234
Fragments arranged by size:
778 234 32
PstI C_TGCA'G
Cuts at: 0 212 434 1044
Size: 212 222 610
Fragments arranged by size:
610 222 212
PvuII CAG'CTG
Cuts at: 0 774 1044
Size: 774 270
SacII CC_GC'GG
Cuts at: 0 497 713 1044
Size: 497 216 331
Fragments arranged by size:
497 331 216
SfcI C'TryA_G
Cuts at: 0 208 430 618 1044
Size: 208 222 188 426
Fragments arranged by size:
426 222 208 188
SfoI GGC'GCC
Cuts at: 0 708 1044
Size: 708 336
SmaI CCC'GGG
Cuts at: 0 671 1044
Size: 671 373
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 838 1044
Size: 838 206
XmaI C'CCGG_G
Cuts at: 0 669 1044
Size: 669 375
XmnI GAAnn'nnTTC
Cuts at: 0 793 1044
Size: 793 251
Enzymes that do cut:
AarI AccI AcuI AfeI AlwNI AvaI BanI BanII
BbeI BbsI BglI BmgBI BplI BpmI BsaI BsaBI
BsaHI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BspMI
BsrDI BsrFI BstAPI BstYI BtgI BtgZI BtsI EaeI
EagI EciI Eco57MI EcoNI HaeII Hin4I KasI MscI
MslI MspA1I NarI PflMI PfoI PpiI PstI PvuII
SacII SfcI SfoI SmaI TaqII XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BmtI Bpu10I BpuEI BsaAI
BsaWI BsaXI BsiHKAI BsiWI BsmI BspEI BspHI BsrBI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EarI EcoICRI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TatI TstI
Tth111I XbaI XcmI XhoI ZraI