(Linear) (Six-base) MAPSORT of: KIBB9700.seq  Check: 3499  from: 1  to: 888



KIBB9700.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     492     572     660     828     888 
   Size:        492      80      88     168      60

  Fragments arranged by size: 

                 492     168      88      80      60
 
AflIII A'CryG_T

Cuts at:      0     736     888 
   Size:        736     152
 
AleI CACnn'nnGTG

Cuts at:      0     803     888 
   Size:        803      85
 
ApoI r'AATT_y

Cuts at:      0     542     690     757     888 
   Size:        542     148      67     131

  Fragments arranged by size: 

                 542     148     131      67
 
BamHI G'GATC_C

Cuts at:      0      33     888 
   Size:         33     855
 
BanI G'GyrC_C

Cuts at:      0     374     888 
   Size:        374     514
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     180     888 
   Size:        180     708
 
BglII A'GATC_T

Cuts at:      0     606     888 
   Size:        606     282
 
BsaHI Gr'CG_yC

Cuts at:      0     287     888 
   Size:        287     601
 
BsaWI w'CCGG_w

Cuts at:      0     788     888 
   Size:        788     100
 
BsiHKAI G_wGCw'C

Cuts at:      0     113     888 
   Size:        113     775
 
Bsp1286I G_dGCh'C

Cuts at:      0     113     888 
   Size:        113     775
 
BspHI T'CATG_A

Cuts at:      0     741     888 
   Size:        741     147
 
BstYI r'GATC_y

Cuts at:      0      33     606     888 
   Size:         33     573     282

  Fragments arranged by size: 

                 573     282      33
 
BtgI C'CryG_G

Cuts at:      0     157     888 
   Size:        157     731
 
DraI TTT'AAA

Cuts at:      0     483     888 
   Size:        483     405
 
DraIII CAC_nnn'GTG

Cuts at:      0     363     888 
   Size:        363     525
 
EarI CTCTTCn'nnn_

Cuts at:      0     146     548     888 
   Size:        146     402     340

  Fragments arranged by size: 

                 402     340     146
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     492     572     660     828     888 
   Size:        492      80      88     168      60

  Fragments arranged by size: 

                 492     168      88      80      60
 
EcoO109I rG'GnC_Cy

Cuts at:      0      48     888 
   Size:         48     840
 
EcoRI G'AATT_C

Cuts at:      0     542     888 
   Size:        542     346
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     120     152     591     623     888 
   Size:        120      32     439      32     265

  Fragments arranged by size: 

                 439     265     120      32      32
 
HincII GTy'rAC

Cuts at:      0     574     888 
   Size:        574     314
 
HindIII A'AGCT_T

Cuts at:      0     672     888 
   Size:        672     216
 
MslI CAynn'nnrTG

Cuts at:      0     651     746     803     888 
   Size:        651      95      57      85

  Fragments arranged by size: 

                 651      95      85      57
 
NcoI C'CATG_G

Cuts at:      0     157     888 
   Size:        157     731
 
NdeI CA'TA_TG

Cuts at:      0     829     888 
   Size:        829      59
 
NspI r_CATG'y

Cuts at:      0     740     888 
   Size:        740     148
 
PciI A'CATG_T

Cuts at:      0     736     888 
   Size:        736     152
 
SapI GCTCTTCn'nnn_

Cuts at:      0     146     888 
   Size:        146     742
 
SfcI C'TryA_G

Cuts at:      0     206     888 
   Size:        206     682
 
StyI C'CwwG_G

Cuts at:      0     157     633     888 
   Size:        157     476     255

  Fragments arranged by size: 

                 476     255     157
 
XbaI T'CTAG_A

Cuts at:      0     138     888 
   Size:        138     750
 
XmnI GAAnn'nnTTC

Cuts at:      0     505     673     888 
   Size:        505     168     215

  Fragments arranged by size: 

                 505     215     168
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     ApoI    BamHI     BanI    BciVI    BglII
    BsaHI    BsaWI  BsiHKAI Bsp1286I    BspHI    BstYI     BtgI     DraI
   DraIII     EarI  Eco57MI EcoO109I    EcoRI    Hin4I   HincII  HindIII
     MslI     NcoI     NdeI     NspI     PciI     SapI     SfcI     StyI
     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BanII     BbeI     BbsI    BbvCI
     BcgI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI
     XmaI     ZraI