Restriction map for
pF1KB9698
(Linear) (Six-base) MAPSORT of: KIBB9698.seq Check: 6519 from: 1 to: 873
KIBB9698.orf
With 182 enzymes: SgfI *
February 25, 2010 13:26 ..
AccI GT'mk_AC
Cuts at: 0 102 136 873
Size: 102 34 737
Fragments arranged by size:
737 102 34
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 764 873
Size: 764 109
AleI CACnn'nnGTG
Cuts at: 0 127 873
Size: 127 746
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 104 873
Size: 104 769
AlwNI CAG_nnn'CTG
Cuts at: 0 313 873
Size: 313 560
ApoI r'AATT_y
Cuts at: 0 45 238 412 490 873
Size: 45 193 174 78 383
Fragments arranged by size:
383 193 174 78 45
BciVI GTATCCnnnnn_n'
Cuts at: 0 433 873
Size: 433 440
BfrBI ATG'CAT
Cuts at: 0 81 547 873
Size: 81 466 326
Fragments arranged by size:
466 326 81
BlpI GC'TnA_GC
Cuts at: 0 532 590 873
Size: 532 58 283
Fragments arranged by size:
532 283 58
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 601 706 778 873
Size: 362 239 105 72 95
Fragments arranged by size:
362 239 105 95 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 276 707 873
Size: 276 431 166
Fragments arranged by size:
431 276 166
BsaHI Gr'CG_yC
Cuts at: 0 53 873
Size: 53 820
BsaWI w'CCGG_w
Cuts at: 0 791 873
Size: 791 82
BsmBI CGTCTCn'nnnn_
Cuts at: 0 417 873
Size: 417 456
BspHI T'CATG_A
Cuts at: 0 731 873
Size: 731 142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 202 873
Size: 202 671
BsrDI GCAATG_nn'
Cuts at: 0 665 873
Size: 665 208
BsrGI T'GTAC_A
Cuts at: 0 608 873
Size: 608 265
BstXI CCAn_nnnn'nTGG
Cuts at: 0 579 873
Size: 579 294
DraI TTT'AAA
Cuts at: 0 417 540 873
Size: 417 123 333
Fragments arranged by size:
417 333 123
EarI CTCTTCn'nnn_
Cuts at: 0 293 350 873
Size: 293 57 523
Fragments arranged by size:
523 293 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 351 873
Size: 351 522
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 601 706 764 778 873
Size: 362 239 105 58 14 95
Fragments arranged by size:
362 239 105 95 58 14
EcoO109I rG'GnC_Cy
Cuts at: 0 300 465 873
Size: 300 165 408
Fragments arranged by size:
408 300 165
EcoRI G'AATT_C
Cuts at: 0 45 490 873
Size: 45 445 383
Fragments arranged by size:
445 383 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 66 98 873
Size: 66 32 775
Fragments arranged by size:
775 66 32
HindIII A'AGCT_T
Cuts at: 0 11 873
Size: 11 862
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 873
Size: 137 736
MslI CAynn'nnrTG
Cuts at: 0 86 127 736 873
Size: 86 41 609 137
Fragments arranged by size:
609 137 86 41
MspA1I CmG'CkG
Cuts at: 0 310 475 873
Size: 310 165 398
Fragments arranged by size:
398 310 165
NsiI A_TGCA'T
Cuts at: 0 83 549 873
Size: 83 466 324
Fragments arranged by size:
466 324 83
NspI r_CATG'y
Cuts at: 0 551 873
Size: 551 322
PpuMI rG'GwC_Cy
Cuts at: 0 300 873
Size: 300 573
PsiI TTA'TAA
Cuts at: 0 236 873
Size: 236 637
PstI C_TGCA'G
Cuts at: 0 530 873
Size: 530 343
PvuII CAG'CTG
Cuts at: 0 310 475 873
Size: 310 165 398
Fragments arranged by size:
398 310 165
SapI GCTCTTCn'nnn_
Cuts at: 0 350 873
Size: 350 523
SfcI C'TryA_G
Cuts at: 0 137 526 873
Size: 137 389 347
Fragments arranged by size:
389 347 137
SmlI C'TyrA_G
Cuts at: 0 255 722 862 873
Size: 255 467 140 11
Fragments arranged by size:
467 255 140 11
SphI G_CATG'C
Cuts at: 0 551 873
Size: 551 322
SspI AAT'ATT
Cuts at: 0 566 873
Size: 566 307
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 460 873
Size: 460 413
TatI w'GTAC_w
Cuts at: 0 608 873
Size: 608 265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 454 873
Size: 454 419
Enzymes that do cut:
AccI AcuI AleI AlfI AlwNI ApoI BciVI BfrBI
BlpI BpmI BpuEI BsaHI BsaWI BsmBI BspHI BspMI
BsrDI BsrGI BstXI DraI EarI EciI Eco57MI EcoO109I
EcoRI Hin4I HindIII MmeI MslI MspA1I NsiI NspI
PpuMI PsiI PstI PvuII SapI SfcI SmlI SphI
SspI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BclI BglI BglII Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BsaI BsaAI BsaBI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII DrdI
EaeI EagI EcoICRI EcoNI EcoRV FalI FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI StuI StyI
SwaI TstI Tth111I XbaI XhoI XmaI XmnI ZraI