Restriction map for
pF1KB9692
(Linear) (Six-base) MAPSORT of: KIBB9692.seq Check: 7506 from: 1 to: 729
KIBB9692.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AleI CACnn'nnGTG
Cuts at: 0 112 729
Size: 112 617
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 475 507 729
Size: 475 32 222
Fragments arranged by size:
475 222 32
AlwNI CAG_nnn'CTG
Cuts at: 0 93 474 672 729
Size: 93 381 198 57
Fragments arranged by size:
381 198 93 57
ApaI G_GGCC'C
Cuts at: 0 684 729
Size: 684 45
BamHI G'GATC_C
Cuts at: 0 300 729
Size: 300 429
BanII G_rGCy'C
Cuts at: 0 7 373 385 684 729
Size: 7 366 12 299 45
Fragments arranged by size:
366 299 45 12 7
BglI GCCn_nnn'nGGC
Cuts at: 0 688 729
Size: 688 41
Bme1580I G_kGCm'C
Cuts at: 0 684 729
Size: 684 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 502 679 694 729
Size: 496 6 177 15 35
Fragments arranged by size:
496 177 35 15 6
BsaWI w'CCGG_w
Cuts at: 0 587 729
Size: 587 142
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 475 505 729
Size: 475 30 224
Fragments arranged by size:
475 224 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 373 667 729
Size: 373 294 62
Fragments arranged by size:
373 294 62
BseYI C'CCAG_C
Cuts at: 0 124 631 729
Size: 124 507 98
Fragments arranged by size:
507 124 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 729
Size: 334 395
BsiEI CG_ry'CG
Cuts at: 0 102 729
Size: 102 627
BsiHKAI G_wGCw'C
Cuts at: 0 7 729
Size: 7 722
Bsp1286I G_dGCh'C
Cuts at: 0 7 373 385 684 729
Size: 7 366 12 299 45
Fragments arranged by size:
366 299 45 12 7
BsrBI CCG'CTC
Cuts at: 0 678 729
Size: 678 51
BstAPI GCAn_nnn'nTGC
Cuts at: 0 641 729
Size: 641 88
BstXI CCAn_nnnn'nTGG
Cuts at: 0 351 729
Size: 351 378
BstYI r'GATC_y
Cuts at: 0 300 729
Size: 300 429
BtgI C'CryG_G
Cuts at: 0 110 174 194 224 234 729
Size: 110 64 20 30 10 495
Fragments arranged by size:
495 110 64 30 20 10
EaeI y'GGCC_r
Cuts at: 0 99 729
Size: 99 630
EagI C'GGCC_G
Cuts at: 0 99 729
Size: 99 630
EarI CTCTTCn'nnn_
Cuts at: 0 406 729
Size: 406 323
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 709 729
Size: 709 20
EcoICRI GAG'CTC
Cuts at: 0 5 729
Size: 5 724
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 502 679 694 729
Size: 496 6 177 15 35
Fragments arranged by size:
496 177 35 15 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 355 729
Size: 355 374
EcoO109I rG'GnC_Cy
Cuts at: 0 90 240 681 729
Size: 90 150 441 48
Fragments arranged by size:
441 150 90 48
FspI TGC'GCA
Cuts at: 0 296 729
Size: 296 433
HincII GTy'rAC
Cuts at: 0 18 729
Size: 18 711
HindIII A'AGCT_T
Cuts at: 0 331 729
Size: 331 398
MslI CAynn'nnrTG
Cuts at: 0 112 729
Size: 112 617
MspA1I CmG'CkG
Cuts at: 0 131 270 347 729
Size: 131 139 77 382
Fragments arranged by size:
382 139 131 77
PasI CC'CwG_GG
Cuts at: 0 546 729
Size: 546 183
PflMI CCAn_nnn'nTGG
Cuts at: 0 93 474 729
Size: 93 381 255
Fragments arranged by size:
381 255 93
PfoI T'CCnGG_A
Cuts at: 0 161 729
Size: 161 568
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 475 507 729
Size: 475 32 222
Fragments arranged by size:
475 222 32
PpuMI rG'GwC_Cy
Cuts at: 0 90 240 729
Size: 90 150 489
Fragments arranged by size:
489 150 90
PspOMI G'GGCC_C
Cuts at: 0 680 729
Size: 680 49
SacI G_AGCT'C
Cuts at: 0 7 729
Size: 7 722
SanDI GG'GwC_CC
Cuts at: 0 240 729
Size: 240 489
SapI GCTCTTCn'nnn_
Cuts at: 0 406 729
Size: 406 323
SexAI A'CCwGG_T
Cuts at: 0 308 729
Size: 308 421
SfcI C'TryA_G
Cuts at: 0 456 729
Size: 456 273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 386 418 729
Size: 386 32 311
Fragments arranged by size:
386 311 32
XmnI GAAnn'nnTTC
Cuts at: 0 490 729
Size: 490 239
Enzymes that do cut:
AleI AloI AlwNI ApaI BamHI BanII BglI Bme1580I
BpmI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BsrBI BstAPI BstXI BstYI BtgI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HincII
HindIII MslI MspA1I PasI PflMI PfoI PpiI PpuMI
PspOMI SacI SanDI SapI SexAI SfcI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EcoRI EcoRV
FalI FseI FspAI HaeII Hin4I HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TatI Tth111I XbaI
XcmI XhoI XmaI ZraI