Restriction map for
pF1KB9689
(Linear) (Six-base) MAPSORT of: KIBB9689.seq Check: 2916 from: 1 to: 690
KIBB9689.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
ApaLI G'TGCA_C
Cuts at: 0 505 690
Size: 505 185
AscI GG'CGCG_CC
Cuts at: 0 578 690
Size: 578 112
AvaI C'yCGr_G
Cuts at: 0 8 128 690
Size: 8 120 562
Fragments arranged by size:
562 120 8
BanI G'GyrC_C
Cuts at: 0 68 585 639 690
Size: 68 517 54 51
Fragments arranged by size:
517 68 54 51
BanII G_rGCy'C
Cuts at: 0 221 690
Size: 221 469
BbeI G_GCGC'C
Cuts at: 0 72 589 643 690
Size: 72 517 54 47
Fragments arranged by size:
517 72 54 47
BbvCI CC'TCA_GC
Cuts at: 0 103 690
Size: 103 587
BglI GCCn_nnn'nGGC
Cuts at: 0 232 349 469 690
Size: 232 117 120 221
Fragments arranged by size:
232 221 120 117
Bme1580I G_kGCm'C
Cuts at: 0 509 690
Size: 509 181
BmrI ACTGGGnnnn_n'
Cuts at: 0 250 496 690
Size: 250 246 194
Fragments arranged by size:
250 246 194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 178 221 419 690
Size: 139 39 43 198 271
Fragments arranged by size:
271 198 139 43 39
Bpu10I CC'TnA_GC
Cuts at: 0 103 690
Size: 103 587
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 216 690
Size: 31 185 474
Fragments arranged by size:
474 185 31
BsaI GGTCTCn'nnnn_
Cuts at: 0 672 690
Size: 672 18
BsaHI Gr'CG_yC
Cuts at: 0 69 266 341 586 640 690
Size: 69 197 75 245 54 50
Fragments arranged by size:
245 197 75 69 54 50
BsaWI w'CCGG_w
Cuts at: 0 143 295 595 690
Size: 143 152 300 95
Fragments arranged by size:
300 152 143 95
BseYI C'CCAG_C
Cuts at: 0 362 690
Size: 362 328
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 472 690
Size: 364 108 218
Fragments arranged by size:
364 218 108
BsiEI CG_ry'CG
Cuts at: 0 227 353 690
Size: 227 126 337
Fragments arranged by size:
337 227 126
BsiHKAI G_wGCw'C
Cuts at: 0 509 690
Size: 509 181
Bsp1286I G_dGCh'C
Cuts at: 0 221 509 690
Size: 221 288 181
Fragments arranged by size:
288 221 181
BspEI T'CCGG_A
Cuts at: 0 143 690
Size: 143 547
BsrBI CCG'CTC
Cuts at: 0 123 690
Size: 123 567
BsrFI r'CCGG_y
Cuts at: 0 417 468 582 690
Size: 417 51 114 108
Fragments arranged by size:
417 114 108 51
BssHII G'CGCG_C
Cuts at: 0 561 578 690
Size: 561 17 112
Fragments arranged by size:
561 112 17
BstEII G'GTnAC_C
Cuts at: 0 3 690
Size: 3 687
BstYI r'GATC_y
Cuts at: 0 423 690
Size: 423 267
BtgI C'CryG_G
Cuts at: 0 185 230 690
Size: 185 45 460
Fragments arranged by size:
460 185 45
EaeI y'GGCC_r
Cuts at: 0 224 350 690
Size: 224 126 340
Fragments arranged by size:
340 224 126
EagI C'GGCC_G
Cuts at: 0 224 350 690
Size: 224 126 340
Fragments arranged by size:
340 224 126
EarI CTCTTCn'nnn_
Cuts at: 0 92 373 690
Size: 92 281 317
Fragments arranged by size:
317 281 92
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 178 221 419 690
Size: 139 39 43 198 271
Fragments arranged by size:
271 198 139 43 39
EcoO109I rG'GnC_Cy
Cuts at: 0 648 690
Size: 648 42
FspI TGC'GCA
Cuts at: 0 534 690
Size: 534 156
HaeII r_GCGC'y
Cuts at: 0 72 277 442 498 589 643 690
Size: 72 205 165 56 91 54 47
Fragments arranged by size:
205 165 91 72 56 54 47
KasI G'GCGC_C
Cuts at: 0 68 585 639 690
Size: 68 517 54 51
Fragments arranged by size:
517 68 54 51
MspA1I CmG'CkG
Cuts at: 0 232 284 654 690
Size: 232 52 370 36
Fragments arranged by size:
370 232 52 36
NaeI GCC'GGC
Cuts at: 0 584 690
Size: 584 106
NarI GG'CG_CC
Cuts at: 0 69 586 640 690
Size: 69 517 54 50
Fragments arranged by size:
517 69 54 50
NgoMIV G'CCGG_C
Cuts at: 0 582 690
Size: 582 108
PfoI T'CCnGG_A
Cuts at: 0 260 690
Size: 260 430
PvuII CAG'CTG
Cuts at: 0 284 690
Size: 284 406
SacII CC_GC'GG
Cuts at: 0 233 690
Size: 233 457
SapI GCTCTTCn'nnn_
Cuts at: 0 92 373 690
Size: 92 281 317
Fragments arranged by size:
317 281 92
SfoI GGC'GCC
Cuts at: 0 70 587 641 690
Size: 70 517 54 49
Fragments arranged by size:
517 70 54 49
SgrAI Cr'CCGG_yG
Cuts at: 0 582 690
Size: 582 108
SmaI CCC'GGG
Cuts at: 0 10 130 690
Size: 10 120 560
Fragments arranged by size:
560 120 10
SmlI C'TyrA_G
Cuts at: 0 46 195 690
Size: 46 149 495
Fragments arranged by size:
495 149 46
StuI AGG'CCT
Cuts at: 0 432 690
Size: 432 258
StyI C'CwwG_G
Cuts at: 0 474 644 682 690
Size: 474 170 38 8
Fragments arranged by size:
474 170 38 8
XmaI C'CCGG_G
Cuts at: 0 8 128 690
Size: 8 120 562
Fragments arranged by size:
562 120 8
Enzymes that do cut:
ApaLI AscI AvaI BanI BanII BbeI BbvCI BglI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BsrBI BsrFI
BssHII BstEII BstYI BtgI EaeI EagI EarI Eco57MI
EcoO109I FspI HaeII KasI MspA1I NaeI NarI NgoMIV
PfoI PvuII SacII SapI SfoI SgrAI SmaI SmlI
StuI StyI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApoI AseI AsiSI AvrII BaeI BamHI BbsI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI
BsaAI BsaBI BsaXI BseRI BsiWI BsmI BsmBI BspHI
BspMI BsrDI BsrGI BssSI BstAPI BstBI BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SalI SanDI SbfI ScaI SexAI SfcI
SfiI SgfI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI
ZraI