Restriction map for
pF1KB9688
(Linear) (Six-base) MAPSORT of: KIBB9688.seq Check: 9916 from: 1 to: 666
KIBB9688.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AccI GT'mk_AC
Cuts at: 0 182 666
Size: 182 484
AflII C'TTAA_G
Cuts at: 0 132 666
Size: 132 534
AlwNI CAG_nnn'CTG
Cuts at: 0 113 501 666
Size: 113 388 165
Fragments arranged by size:
388 165 113
ApoI r'AATT_y
Cuts at: 0 517 666
Size: 517 149
AvaI C'yCGr_G
Cuts at: 0 200 286 508 666
Size: 200 86 222 158
Fragments arranged by size:
222 200 158 86
BanII G_rGCy'C
Cuts at: 0 7 40 207 666
Size: 7 33 167 459
Fragments arranged by size:
459 167 33 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 543 577 666
Size: 543 34 89
Fragments arranged by size:
543 89 34
BglII A'GATC_T
Cuts at: 0 599 666
Size: 599 67
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 666
Size: 343 323
BsaAI yAC'GTr
Cuts at: 0 12 666
Size: 12 654
BsaBI GATnn'nnATC
Cuts at: 0 598 666
Size: 598 68
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 266 340 373 666
Size: 266 74 33 293
Fragments arranged by size:
293 266 74 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 424 666
Size: 118 306 242
Fragments arranged by size:
306 242 118
BsiEI CG_ry'CG
Cuts at: 0 216 239 666
Size: 216 23 427
Fragments arranged by size:
427 216 23
BsiHKAI G_wGCw'C
Cuts at: 0 7 666
Size: 7 659
BsmBI CGTCTCn'nnnn_
Cuts at: 0 257 448 666
Size: 257 191 218
Fragments arranged by size:
257 218 191
Bsp1286I G_dGCh'C
Cuts at: 0 7 40 207 666
Size: 7 33 167 459
Fragments arranged by size:
459 167 33 7
BsrBI CCG'CTC
Cuts at: 0 241 666
Size: 241 425
BsrFI r'CCGG_y
Cuts at: 0 402 666
Size: 402 264
BsrGI T'GTAC_A
Cuts at: 0 293 666
Size: 293 373
BssSI C'ACGA_G
Cuts at: 0 451 666
Size: 451 215
BstYI r'GATC_y
Cuts at: 0 599 666
Size: 599 67
BtgI C'CryG_G
Cuts at: 0 174 233 424 666
Size: 174 59 191 242
Fragments arranged by size:
242 191 174 59
BtsI GCAGTG_nn'
Cuts at: 0 348 666
Size: 348 318
EaeI y'GGCC_r
Cuts at: 0 236 666
Size: 236 430
EagI C'GGCC_G
Cuts at: 0 236 666
Size: 236 430
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 137 666
Size: 137 529
EcoICRI GAG'CTC
Cuts at: 0 5 666
Size: 5 661
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 666
Size: 343 323
EcoRI G'AATT_C
Cuts at: 0 517 666
Size: 517 149
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 158 666
Size: 158 508
HaeII r_GCGC'y
Cuts at: 0 233 322 666
Size: 233 89 344
Fragments arranged by size:
344 233 89
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 282 314 666
Size: 282 32 352
Fragments arranged by size:
352 282 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 666
Size: 188 478
MspA1I CmG'CkG
Cuts at: 0 235 666
Size: 235 431
NotI GC'GGCC_GC
Cuts at: 0 236 666
Size: 236 430
NspI r_CATG'y
Cuts at: 0 63 666
Size: 63 603
PflMI CCAn_nnn'nTGG
Cuts at: 0 501 666
Size: 501 165
PfoI T'CCnGG_A
Cuts at: 0 243 666
Size: 243 423
PstI C_TGCA'G
Cuts at: 0 229 666
Size: 229 437
SacI G_AGCT'C
Cuts at: 0 7 666
Size: 7 659
SacII CC_GC'GG
Cuts at: 0 236 666
Size: 236 430
SfcI C'TryA_G
Cuts at: 0 225 666
Size: 225 441
SmaI CCC'GGG
Cuts at: 0 202 288 666
Size: 202 86 378
Fragments arranged by size:
378 202 86
SmlI C'TyrA_G
Cuts at: 0 132 508 666
Size: 132 376 158
Fragments arranged by size:
376 158 132
SnaBI TAC'GTA
Cuts at: 0 12 666
Size: 12 654
TatI w'GTAC_w
Cuts at: 0 293 666
Size: 293 373
XhoI C'TCGA_G
Cuts at: 0 508 666
Size: 508 158
XmaI C'CCGG_G
Cuts at: 0 200 286 666
Size: 200 86 380
Fragments arranged by size:
380 200 86
XmnI GAAnn'nnTTC
Cuts at: 0 517 666
Size: 517 149
Enzymes that do cut:
AccI AflII AlwNI ApoI AvaI BanII BcgI BglII
BpmI BsaAI BsaBI BseRI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BsrBI BsrFI BsrGI BssSI BstYI BtgI BtsI
EaeI EagI EciI EcoICRI Eco57MI EcoRI FalI HaeII
Hin4I MmeI MspA1I NotI NspI PflMI PfoI PstI
SacI SacII SfcI SmaI SmlI SnaBI TatI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseYI BsiWI BsmI BspEI BspHI BspMI BsrDI BssHII
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EarI EcoNI EcoO109I EcoRV
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MscI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TstI Tth111I XbaI
XcmI ZraI