(Linear) (Six-base) MAPSORT of: KIBB9678.seq  Check: 9114  from: 1  to: 324



KIBB9678.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     257     324 
   Size:        257      67
 
BanII G_rGCy'C

Cuts at:      0     230     324 
   Size:        230      94
 
BbvCI CC'TCA_GC

Cuts at:      0      73     324 
   Size:         73     251
 
BglII A'GATC_T

Cuts at:      0      11     324 
   Size:         11     313
 
BlpI GC'TnA_GC

Cuts at:      0     112     324 
   Size:        112     212
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     280     324 
   Size:         83     197      44

  Fragments arranged by size: 

                 197      83      44
 
Bpu10I CC'TnA_GC

Cuts at:      0      73     209     324 
   Size:         73     136     115

  Fragments arranged by size: 

                 136     115      73
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     221     298     324 
   Size:        221      77      26

  Fragments arranged by size: 

                 221      77      26
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      81     111     324 
   Size:         81      30     213

  Fragments arranged by size: 

                 213      81      30
 
Bsp1286I G_dGCh'C

Cuts at:      0     230     324 
   Size:        230      94
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     257     324 
   Size:        257      67
 
BsrBI CCG'CTC

Cuts at:      0      33     324 
   Size:         33     291
 
BsrDI GCAATG_nn'

Cuts at:      0     146     324 
   Size:        146     178
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     186     324 
   Size:        186     138
 
BstYI r'GATC_y

Cuts at:      0      11     324 
   Size:         11     313
 
EarI CTCTTCn'nnn_

Cuts at:      0     235     324 
   Size:        235      89
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     280     324 
   Size:         83     197      44

  Fragments arranged by size: 

                 197      83      44
 
EcoO109I rG'GnC_Cy

Cuts at:      0     241     324 
   Size:        241      83
 
HaeII r_GCGC'y

Cuts at:      0      62     324 
   Size:         62     262
 
HincII GTy'rAC

Cuts at:      0      54     324 
   Size:         54     270
 
PpuMI rG'GwC_Cy

Cuts at:      0     241     324 
   Size:        241      83
 
SapI GCTCTTCn'nnn_

Cuts at:      0     235     324 
   Size:        235      89
 
SexAI A'CCwGG_T

Cuts at:      0     287     300     324 
   Size:        287      13      24

  Fragments arranged by size: 

                 287      24      13
 
SmlI C'TyrA_G

Cuts at:      0     236     277     324 
   Size:        236      41      47

  Fragments arranged by size: 

                 236      47      41
 
StuI AGG'CCT

Cuts at:      0     257     324 
   Size:        257      67
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      81     113     324 
   Size:         81      32     211

  Fragments arranged by size: 

                 211      81      32
 
XmnI GAAnn'nnTTC

Cuts at:      0      13     128     324 
   Size:         13     115     196

  Fragments arranged by size: 

                 196     115      13
 

 Enzymes that do cut:

     AarI    BanII    BbvCI    BglII     BlpI     BpmI   Bpu10I    BpuEI
    BsaXI Bsp1286I    BspMI    BsrBI    BsrDI   BstAPI    BstYI     EarI
  Eco57MI EcoO109I    HaeII   HincII    PpuMI     SapI    SexAI     SmlI
     StuI     TstI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StyI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI