(Linear) (Six-base) MAPSORT of: KIBB9675.seq  Check: 6211  from: 1  to: 252



KIBB9675.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     137     252 
   Size:        106      31     115

  Fragments arranged by size: 

                 115     106      31
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      57     252 
   Size:         57     195
 
BanII G_rGCy'C

Cuts at:      0     157     252 
   Size:        157      95
 
BmgBI CAC'GTC

Cuts at:      0     193     252 
   Size:        193      59
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     252 
   Size:        196      56
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211     252 
   Size:        211      41
 
BsaAI yAC'GTr

Cuts at:      0      66     252 
   Size:         66     186
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     209     252 
   Size:        209      43
 
Bsp1286I G_dGCh'C

Cuts at:      0     157     252 
   Size:        157      95
 
BsrFI r'CCGG_y

Cuts at:      0      56     252 
   Size:         56     196
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      96     252 
   Size:         96     156
 
BtsI GCAGTG_nn'

Cuts at:      0      41     252 
   Size:         41     211
 
EaeI y'GGCC_r

Cuts at:      0     128     252 
   Size:        128     124
 
EarI CTCTTCn'nnn_

Cuts at:      0     113     227     252 
   Size:        113     114      25

  Fragments arranged by size: 

                 114     113      25
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     137     196     252 
   Size:        106      31      59      56

  Fragments arranged by size: 

                 106      59      56      31
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     178     252 
   Size:        178      74
 
NaeI GCC'GGC

Cuts at:      0      58     252 
   Size:         58     194
 
NgoMIV G'CCGG_C

Cuts at:      0      56     252 
   Size:         56     196
 
SmlI C'TyrA_G

Cuts at:      0     226     252 
   Size:        226      26
 
XmnI GAAnn'nnTTC

Cuts at:      0     213     252 
   Size:        213      39
 

 Enzymes that do cut:

     AcuI     AlfI    BanII    BmgBI     BpmI    BpuEI    BsaAI    BseRI
 Bsp1286I    BsrFI   BstAPI     BtsI     EaeI     EarI  Eco57MI    EcoNI
     NaeI   NgoMIV     SmlI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I     BmrI     BmtI     BplI   Bpu10I     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI