Restriction map for
pF1KB9671
(Linear) (Six-base) MAPSORT of: KIBB9671.seq Check: 4491 from: 1 to: 1548
KIBB9671.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 876 960 1044 1212 1548
Size: 119 757 84 84 168 336
Fragments arranged by size:
757 336 168 119 84 84
AflIII A'CryG_T
Cuts at: 0 1540 1548
Size: 1540 8
ApoI r'AATT_y
Cuts at: 0 422 563 590 647 926 1152 1236 1262
Size: 422 141 27 57 279 226 84 26
Cuts at: 1262 1346 1548
Size: 84 202
Fragments arranged by size:
422 279 226 202 141 84 84 57
27 26
BbsI GAAGACnn'nnnn_
Cuts at: 0 462 630 798 882 966 1050 1218 1302
Size: 462 168 168 84 84 84 168 84
Cuts at: 1302 1548
Size: 246
Fragments arranged by size:
462 246 168 168 168 84 84 84
84
BclI T'GATC_A
Cuts at: 0 120 755 1548
Size: 120 635 793
Fragments arranged by size:
793 635 120
BfrBI ATG'CAT
Cuts at: 0 3 1548
Size: 3 1545
BglII A'GATC_T
Cuts at: 0 383 1548
Size: 383 1165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 536 704 788 872 942 956 1026
Size: 452 84 168 84 84 70 14 70
Cuts at: 1026 1040 1208 1292 1376 1460 1548
Size: 14 168 84 84 84 88
Fragments arranged by size:
452 168 168 88 84 84 84 84
84 84 70 70 14 14
BsaWI w'CCGG_w
Cuts at: 0 598 1548
Size: 598 950
BsmI GAATG_Cn'
Cuts at: 0 1141 1548
Size: 1141 407
BsmBI CGTCTCn'nnnn_
Cuts at: 0 346 1548
Size: 346 1202
BsrFI r'CCGG_y
Cuts at: 0 1102 1548
Size: 1102 446
BsrGI T'GTAC_A
Cuts at: 0 744 1164 1548
Size: 744 420 384
Fragments arranged by size:
744 420 384
BstYI r'GATC_y
Cuts at: 0 383 1548
Size: 383 1165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 484 1548
Size: 484 1064
DraI TTT'AAA
Cuts at: 0 363 1548
Size: 363 1185
EarI CTCTTCn'nnn_
Cuts at: 0 1483 1548
Size: 1483 65
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 452 536 704 788 872 876 942
Size: 119 333 84 168 84 84 4 66
Cuts at: 942 956 960 1026 1040 1044 1208 1212 1292
Size: 14 4 66 14 4 164 4 80
Cuts at: 1292 1376 1460 1548
Size: 84 84 88
Fragments arranged by size:
333 168 164 119 88 84 84 84
84 84 80 66 66 14 14 4
4 4 4
EcoRI G'AATT_C
Cuts at: 0 422 590 926 1262 1548
Size: 422 168 336 336 286
Fragments arranged by size:
422 336 336 286 168
HincII GTy'rAC
Cuts at: 0 141 1548
Size: 141 1407
HindIII A'AGCT_T
Cuts at: 0 1280 1391 1548
Size: 1280 111 157
Fragments arranged by size:
1280 157 111
MslI CAynn'nnrTG
Cuts at: 0 209 213 447 531 1103 1548
Size: 209 4 234 84 572 445
Fragments arranged by size:
572 445 234 209 84 4
NdeI CA'TA_TG
Cuts at: 0 210 1548
Size: 210 1338
NsiI A_TGCA'T
Cuts at: 0 5 1548
Size: 5 1543
NspI r_CATG'y
Cuts at: 0 1544 1548
Size: 1544 4
PciI A'CATG_T
Cuts at: 0 1540 1548
Size: 1540 8
PsiI TTA'TAA
Cuts at: 0 614 1118 1286 1548
Size: 614 504 168 262
Fragments arranged by size:
614 504 262 168
PstI C_TGCA'G
Cuts at: 0 86 1548
Size: 86 1462
SfcI C'TryA_G
Cuts at: 0 82 1548
Size: 82 1466
SspI AAT'ATT
Cuts at: 0 261 1548
Size: 261 1287
StuI AGG'CCT
Cuts at: 0 301 1548
Size: 301 1247
SwaI ATTT'AAAT
Cuts at: 0 363 1548
Size: 363 1185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1303 1548
Size: 1303 245
TatI w'GTAC_w
Cuts at: 0 744 1164 1548
Size: 744 420 384
Fragments arranged by size:
744 420 384
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 889 921 973 1005 1548
Size: 889 32 52 32 543
Fragments arranged by size:
889 543 52 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 1309 1366 1450 1548
Size: 1309 57 84 98
Fragments arranged by size:
1309 98 84 57
Enzymes that do cut:
AcuI AflIII ApoI BbsI BclI BfrBI BglII BpmI
BsaWI BsmI BsmBI BsrFI BsrGI BstYI BtgZI DraI
EarI Eco57MI EcoRI HincII HindIII MslI NdeI NsiI
NspI PciI PsiI PstI SfcI SspI StuI SwaI
TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BglI BlpI Bme1580I BmgBI
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtsI
ClaI CspCI DraIII DrdI EaeI EagI EciI EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII
Hin4I HpaI KasI KpnI MfeI MluI MmeI MscI
MspA1I NaeI NarI NcoI NgoMIV NheI NotI NruI
PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI StyI Tth111I XbaI XcmI XhoI XmaI ZraI