Restriction map for
pF1KB9610
(Linear) (Six-base) MAPSORT of: KIBB9610.seq Check: 438 from: 1 to: 1569
KIBB9610.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 219 1569
Size: 219 1350
Acc65I G'GTAC_C
Cuts at: 0 1053 1569
Size: 1053 516
AfeI AGC'GCT
Cuts at: 0 1112 1569
Size: 1112 457
AleI CACnn'nnGTG
Cuts at: 0 1198 1569
Size: 1198 371
AlwNI CAG_nnn'CTG
Cuts at: 0 486 666 1377 1392 1462 1569
Size: 486 180 711 15 70 107
Fragments arranged by size:
711 486 180 107 70 15
ApaI G_GGCC'C
Cuts at: 0 1146 1456 1560 1569
Size: 1146 310 104 9
Fragments arranged by size:
1146 310 104 9
ApaLI G'TGCA_C
Cuts at: 0 125 1569
Size: 125 1444
ApoI r'AATT_y
Cuts at: 0 348 1569
Size: 348 1221
AvaI C'yCGr_G
Cuts at: 0 704 1253 1569
Size: 704 549 316
Fragments arranged by size:
704 549 316
BanI G'GyrC_C
Cuts at: 0 464 642 1053 1187 1569
Size: 464 178 411 134 382
Fragments arranged by size:
464 411 382 178 134
BanII G_rGCy'C
Cuts at: 0 626 689 1049 1146 1386 1456 1469 1516
Size: 626 63 360 97 240 70 13 47
Cuts at: 1516 1523 1560 1569
Size: 7 37 9
Fragments arranged by size:
626 360 240 97 70 63 47 37
13 9 7
BlpI GC'TnA_GC
Cuts at: 0 1072 1569
Size: 1072 497
Bme1580I G_kGCm'C
Cuts at: 0 33 129 467 647 1146 1435 1456 1560
Size: 33 96 338 180 499 289 21 104
Cuts at: 1560 1568 1569
Size: 8 1
Fragments arranged by size:
499 338 289 180 104 96 33 21
8 1
BmgBI CAC'GTC
Cuts at: 0 1351 1569
Size: 1351 218
BmrI ACTGGGnnnn_n'
Cuts at: 0 918 1149 1569
Size: 918 231 420
Fragments arranged by size:
918 420 231
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 824 1384 1391 1507 1569
Size: 208 616 560 7 116 62
Fragments arranged by size:
616 560 208 116 62 7
Bpu10I CC'TnA_GC
Cuts at: 0 1385 1463 1569
Size: 1385 78 106
Fragments arranged by size:
1385 106 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 1569
Size: 745 824
BsaI GGTCTCn'nnnn_
Cuts at: 0 175 1569
Size: 175 1394
BsaHI Gr'CG_yC
Cuts at: 0 521 1569
Size: 521 1048
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1358 1569
Size: 1358 211
BseYI C'CCAG_C
Cuts at: 0 1113 1285 1553 1569
Size: 1113 172 268 16
Fragments arranged by size:
1113 268 172 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 1304 1569
Size: 595 709 265
Fragments arranged by size:
709 595 265
BsiHKAI G_wGCw'C
Cuts at: 0 129 689 1078 1207 1386 1569
Size: 129 560 389 129 179 183
Fragments arranged by size:
560 389 183 179 129 129
BsmI GAATG_Cn'
Cuts at: 0 1149 1569
Size: 1149 420
Bsp1286I G_dGCh'C
Cuts at: 0 33 129 467 626 647 689 1049 1078
Size: 33 96 338 159 21 42 360 29
Cuts at: 1078 1146 1207 1386 1435 1456 1469 1516 1523
Size: 68 61 179 49 21 13 47 7
Cuts at: 1523 1560 1568 1569
Size: 37 8 1
Fragments arranged by size:
360 338 179 159 96 68 61 49
47 42 37 33 29 21 21 13
8 7 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 219 494 617 1569
Size: 219 275 123 952
Fragments arranged by size:
952 275 219 123
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1439 1569
Size: 1439 130
BstBI TT'CG_AA
Cuts at: 0 353 1569
Size: 353 1216
BstEII G'GTnAC_C
Cuts at: 0 816 948 1569
Size: 816 132 621
Fragments arranged by size:
816 621 132
BstXI CCAn_nnnn'nTGG
Cuts at: 0 291 1569
Size: 291 1278
Bsu36I CC'TnA_GG
Cuts at: 0 1475 1569
Size: 1475 94
BtgI C'CryG_G
Cuts at: 0 852 959 1569
Size: 852 107 610
Fragments arranged by size:
852 610 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 909 1569
Size: 909 660
BtsI GCAGTG_nn'
Cuts at: 0 1182 1238 1569
Size: 1182 56 331
Fragments arranged by size:
1182 331 56
DraIII CAC_nnn'GTG
Cuts at: 0 735 1569
Size: 735 834
EaeI y'GGCC_r
Cuts at: 0 657 677 1569
Size: 657 20 892
Fragments arranged by size:
892 657 20
EarI CTCTTCn'nnn_
Cuts at: 0 71 694 1212 1569
Size: 71 623 518 357
Fragments arranged by size:
623 518 357 71
EcoICRI GAG'CTC
Cuts at: 0 687 1384 1569
Size: 687 697 185
Fragments arranged by size:
697 687 185
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 824 1384 1391 1507 1569
Size: 208 616 560 7 116 62
Fragments arranged by size:
616 560 208 116 62 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 226 1474 1569
Size: 226 1248 95
Fragments arranged by size:
1248 226 95
EcoO109I rG'GnC_Cy
Cuts at: 0 483 716 1418 1557 1569
Size: 483 233 702 139 12
Fragments arranged by size:
702 483 233 139 12
FspI TGC'GCA
Cuts at: 0 867 976 1569
Size: 867 109 593
Fragments arranged by size:
867 593 109
FspAI rTGC'GCAy
Cuts at: 0 976 1569
Size: 976 593
HaeII r_GCGC'y
Cuts at: 0 666 1114 1569
Size: 666 448 455
Fragments arranged by size:
666 455 448
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 48 80 1569
Size: 48 32 1489
Fragments arranged by size:
1489 48 32
KpnI G_GTAC'C
Cuts at: 0 1057 1569
Size: 1057 512
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 1569
Size: 91 1478
MscI TGG'CCA
Cuts at: 0 659 679 1569
Size: 659 20 890
Fragments arranged by size:
890 659 20
MslI CAynn'nnrTG
Cuts at: 0 289 1198 1569
Size: 289 909 371
Fragments arranged by size:
909 371 289
MspA1I CmG'CkG
Cuts at: 0 1553 1569
Size: 1553 16
NcoI C'CATG_G
Cuts at: 0 852 959 1569
Size: 852 107 610
Fragments arranged by size:
852 610 107
NspI r_CATG'y
Cuts at: 0 552 1442 1569
Size: 552 890 127
Fragments arranged by size:
890 552 127
PasI CC'CwG_GG
Cuts at: 0 1049 1516 1569
Size: 1049 467 53
Fragments arranged by size:
1049 467 53
PflMI CCAn_nnn'nTGG
Cuts at: 0 167 231 632 655 666 1569
Size: 167 64 401 23 11 903
Fragments arranged by size:
903 401 167 64 23 11
PpuMI rG'GwC_Cy
Cuts at: 0 483 1569
Size: 483 1086
PspOMI G'GGCC_C
Cuts at: 0 1142 1452 1556 1569
Size: 1142 310 104 13
Fragments arranged by size:
1142 310 104 13
PstI C_TGCA'G
Cuts at: 0 1230 1569
Size: 1230 339
PvuII CAG'CTG
Cuts at: 0 1553 1569
Size: 1553 16
SacI G_AGCT'C
Cuts at: 0 689 1386 1569
Size: 689 697 183
Fragments arranged by size:
697 689 183
SapI GCTCTTCn'nnn_
Cuts at: 0 694 1212 1569
Size: 694 518 357
Fragments arranged by size:
694 518 357
SfcI C'TryA_G
Cuts at: 0 752 1226 1569
Size: 752 474 343
Fragments arranged by size:
752 474 343
SmaI CCC'GGG
Cuts at: 0 706 1255 1569
Size: 706 549 314
Fragments arranged by size:
706 549 314
SmlI C'TyrA_G
Cuts at: 0 760 1569
Size: 760 809
StyI C'CwwG_G
Cuts at: 0 120 615 852 959 1156 1569
Size: 120 495 237 107 197 413
Fragments arranged by size:
495 413 237 197 120 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 174 1093 1535 1569
Size: 174 919 442 34
Fragments arranged by size:
919 442 174 34
TatI w'GTAC_w
Cuts at: 0 539 1569
Size: 539 1030
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1296 1313 1328 1345 1569
Size: 1296 17 15 17 224
Fragments arranged by size:
1296 224 17 17 15
Tth111I GACn'n_nGTC
Cuts at: 0 1352 1569
Size: 1352 217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 164 931 1339 1569
Size: 164 767 408 230
Fragments arranged by size:
767 408 230 164
XmaI C'CCGG_G
Cuts at: 0 704 1253 1569
Size: 704 549 316
Fragments arranged by size:
704 549 316
Enzymes that do cut:
AarI Acc65I AfeI AleI AlwNI ApaI ApaLI ApoI
AvaI BanI BanII BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaHI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspMI BstAPI BstBI BstEII BstXI Bsu36I
BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I FspI FspAI HaeII Hin4I KpnI MmeI
MscI MslI MspA1I NcoI NspI PasI PflMI PpuMI
PspOMI PstI PvuII SacI SapI SfcI SmaI SmlI
StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AcuI AflII AflIII AgeI AhdI
AlfI AloI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BmtI BplI BsaAI BsaBI BsaWI BsaXI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstYI BstZ17I ClaI CspCI DraI DrdI
EagI EciI EcoRI EcoRV FalI FseI HincII HindIII
HpaI KasI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI XbaI XhoI XmnI ZraI