Restriction map for
pF1KB9546
(Linear) (Six-base) MAPSORT of: KIBB9546.seq Check: 7506 from: 1 to: 1467
KIBB9546.orf
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 988 1467
Size: 988 479
AfeI AGC'GCT
Cuts at: 0 725 1467
Size: 725 742
AleI CACnn'nnGTG
Cuts at: 0 139 502 1467
Size: 139 363 965
Fragments arranged by size:
965 363 139
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1207 1467
Size: 1207 260
AlwNI CAG_nnn'CTG
Cuts at: 0 145 1170 1212 1467
Size: 145 1025 42 255
Fragments arranged by size:
1025 255 145 42
ApaI G_GGCC'C
Cuts at: 0 1326 1406 1467
Size: 1326 80 61
Fragments arranged by size:
1326 80 61
ApaLI G'TGCA_C
Cuts at: 0 1120 1467
Size: 1120 347
AvaI C'yCGr_G
Cuts at: 0 19 255 598 700 984 1467
Size: 19 236 343 102 284 483
Fragments arranged by size:
483 343 284 236 102 19
BanI G'GyrC_C
Cuts at: 0 976 1029 1058 1294 1467
Size: 976 53 29 236 173
Fragments arranged by size:
976 236 173 53 29
BanII G_rGCy'C
Cuts at: 0 407 651 1326 1406 1467
Size: 407 244 675 80 61
Fragments arranged by size:
675 407 244 80 61
BbeI G_GCGC'C
Cuts at: 0 1033 1467
Size: 1033 434
BbsI GAAGACnn'nnnn_
Cuts at: 0 680 1273 1467
Size: 680 593 194
Fragments arranged by size:
680 593 194
BbvCI CC'TCA_GC
Cuts at: 0 1000 1467
Size: 1000 467
BglI GCCn_nnn'nGGC
Cuts at: 0 487 1292 1467
Size: 487 805 175
Fragments arranged by size:
805 487 175
BlpI GC'TnA_GC
Cuts at: 0 106 427 1467
Size: 106 321 1040
Fragments arranged by size:
1040 321 106
Bme1580I G_kGCm'C
Cuts at: 0 1054 1097 1124 1297 1326 1406 1467
Size: 1054 43 27 173 29 80 61
Fragments arranged by size:
1054 173 80 61 43 29 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 1112 1293 1467
Size: 1112 181 174
Fragments arranged by size:
1112 181 174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 844 1030 1234 1279 1342 1467
Size: 844 186 204 45 63 125
Fragments arranged by size:
844 204 186 125 63 45
Bpu10I CC'TnA_GC
Cuts at: 0 650 1000 1467
Size: 650 350 467
Fragments arranged by size:
650 467 350
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 566 1006 1467
Size: 49 517 440 461
Fragments arranged by size:
517 461 440 49
BsaI GGTCTCn'nnnn_
Cuts at: 0 820 1467
Size: 820 647
BsaHI Gr'CG_yC
Cuts at: 0 1030 1237 1467
Size: 1030 207 230
Fragments arranged by size:
1030 230 207
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 185 215 1467
Size: 185 30 1252
Fragments arranged by size:
1252 185 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 532 646 1171 1231 1467
Size: 532 114 525 60 236
Fragments arranged by size:
532 525 236 114 60
BseYI C'CCAG_C
Cuts at: 0 325 330 469 493 1467
Size: 325 5 139 24 974
Fragments arranged by size:
974 325 139 24 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 1267 1467
Size: 607 660 200
Fragments arranged by size:
660 607 200
BsiEI CG_ry'CG
Cuts at: 0 622 1467
Size: 622 845
BsiHKAI G_wGCw'C
Cuts at: 0 607 651 935 1124 1205 1467
Size: 607 44 284 189 81 262
Fragments arranged by size:
607 284 262 189 81 44
Bsp1286I G_dGCh'C
Cuts at: 0 407 607 651 935 1054 1097 1124 1205
Size: 407 200 44 284 119 43 27 81
Cuts at: 1205 1297 1326 1406 1467
Size: 92 29 80 61
Fragments arranged by size:
407 284 200 119 92 81 80 61
44 43 29 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 971 988 1467
Size: 971 17 479
Fragments arranged by size:
971 479 17
BsrBI CCG'CTC
Cuts at: 0 196 780 1221 1467
Size: 196 584 441 246
Fragments arranged by size:
584 441 246 196
BsrDI GCAATG_nn'
Cuts at: 0 480 1467
Size: 480 987
BsrFI r'CCGG_y
Cuts at: 0 14 266 677 1226 1467
Size: 14 252 411 549 241
Fragments arranged by size:
549 411 252 241 14
BssHII G'CGCG_C
Cuts at: 0 43 1467
Size: 43 1424
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1170 1467
Size: 1170 297
BstEII G'GTnAC_C
Cuts at: 0 735 1362 1467
Size: 735 627 105
Fragments arranged by size:
735 627 105
Bsu36I CC'TnA_GG
Cuts at: 0 158 209 1467
Size: 158 51 1258
Fragments arranged by size:
1258 158 51
BtgI C'CryG_G
Cuts at: 0 32 91 305 485 740 1467
Size: 32 59 214 180 255 727
Fragments arranged by size:
727 255 214 180 59 32
BtsI GCAGTG_nn'
Cuts at: 0 134 146 206 438 1191 1250 1390 1467
Size: 134 12 60 232 753 59 140 77
Fragments arranged by size:
753 232 140 134 77 60 59 12
DrdI GACnn_nn'nnGTC
Cuts at: 0 562 1467
Size: 562 905
EaeI y'GGCC_r
Cuts at: 0 182 268 619 1112 1397 1467
Size: 182 86 351 493 285 70
Fragments arranged by size:
493 351 285 182 86 70
EagI C'GGCC_G
Cuts at: 0 619 1467
Size: 619 848
EarI CTCTTCn'nnn_
Cuts at: 0 778 1148 1467
Size: 778 370 319
Fragments arranged by size:
778 370 319
EcoICRI GAG'CTC
Cuts at: 0 649 1467
Size: 649 818
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 844 1030 1234 1279 1342 1467
Size: 844 186 204 45 63 125
Fragments arranged by size:
844 204 186 125 63 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 174 1467
Size: 174 1293
EcoO109I rG'GnC_Cy
Cuts at: 0 154 393 897 989 1323 1403 1467
Size: 154 239 504 92 334 80 64
Fragments arranged by size:
504 334 239 154 92 80 64
HaeII r_GCGC'y
Cuts at: 0 28 376 727 1033 1467
Size: 28 348 351 306 434
Fragments arranged by size:
434 351 348 306 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 622 654 1467
Size: 622 32 813
Fragments arranged by size:
813 622 32
HindIII A'AGCT_T
Cuts at: 0 119 1467
Size: 119 1348
KasI G'GCGC_C
Cuts at: 0 1029 1467
Size: 1029 438
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 875 1467
Size: 875 592
MscI TGG'CCA
Cuts at: 0 1114 1467
Size: 1114 353
MslI CAynn'nnrTG
Cuts at: 0 139 502 1127 1467
Size: 139 363 625 340
Fragments arranged by size:
625 363 340 139
MspA1I CmG'CkG
Cuts at: 0 93 426 815 1004 1212 1353 1467
Size: 93 333 389 189 208 141 114
Fragments arranged by size:
389 333 208 189 141 114 93
NaeI GCC'GGC
Cuts at: 0 16 1467
Size: 16 1451
NarI GG'CG_CC
Cuts at: 0 1030 1467
Size: 1030 437
NcoI C'CATG_G
Cuts at: 0 485 1467
Size: 485 982
NgoMIV G'CCGG_C
Cuts at: 0 14 1467
Size: 14 1453
NotI GC'GGCC_GC
Cuts at: 0 619 1467
Size: 619 848
PasI CC'CwG_GG
Cuts at: 0 1455 1467
Size: 1455 12
PflMI CCAn_nnn'nTGG
Cuts at: 0 1212 1467
Size: 1212 255
PfoI T'CCnGG_A
Cuts at: 0 821 1467
Size: 821 646
PpuMI rG'GwC_Cy
Cuts at: 0 154 897 1467
Size: 154 743 570
Fragments arranged by size:
743 570 154
PspOMI G'GGCC_C
Cuts at: 0 1322 1402 1467
Size: 1322 80 65
Fragments arranged by size:
1322 80 65
PstI C_TGCA'G
Cuts at: 0 11 141 968 1196 1467
Size: 11 130 827 228 271
Fragments arranged by size:
827 271 228 130 11
PvuII CAG'CTG
Cuts at: 0 1004 1212 1353 1467
Size: 1004 208 141 114
Fragments arranged by size:
1004 208 141 114
SacI G_AGCT'C
Cuts at: 0 651 1467
Size: 651 816
SacII CC_GC'GG
Cuts at: 0 94 1467
Size: 94 1373
SfcI C'TryA_G
Cuts at: 0 7 137 810 964 1192 1467
Size: 7 130 673 154 228 275
Fragments arranged by size:
673 275 228 154 130 7
SfoI GGC'GCC
Cuts at: 0 1031 1467
Size: 1031 436
SmaI CCC'GGG
Cuts at: 0 257 986 1467
Size: 257 729 481
Fragments arranged by size:
729 481 257
SmlI C'TyrA_G
Cuts at: 0 64 581 1021 1467
Size: 64 517 440 446
Fragments arranged by size:
517 446 440 64
StuI AGG'CCT
Cuts at: 0 731 1072 1467
Size: 731 341 395
Fragments arranged by size:
731 395 341
StyI C'CwwG_G
Cuts at: 0 485 1034 1467
Size: 485 549 433
Fragments arranged by size:
549 485 433
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 288 844 937 1035 1150 1312 1467
Size: 288 556 93 98 115 162 155
Fragments arranged by size:
556 288 162 155 115 98 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 185 217 1467
Size: 185 32 1250
Fragments arranged by size:
1250 185 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 747 1419 1467
Size: 747 672 48
Fragments arranged by size:
747 672 48
XmaI C'CCGG_G
Cuts at: 0 255 984 1467
Size: 255 729 483
Fragments arranged by size:
729 483 255
Enzymes that do cut:
AarI AfeI AleI AlfI AlwNI ApaI ApaLI AvaI
BanI BanII BbeI BbsI BbvCI BglI BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssHII BstAPI BstEII Bsu36I BtgI BtsI DrdI
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I HindIII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI PasI PflMI PfoI PpuMI
PspOMI PstI PvuII SacI SacII SfcI SfoI SmaI
SmlI StuI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BcgI BciVI BclI BfrBI BglII BmgBI BmtI
BplI BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI
BspHI BsrGI BssSI BstBI BstXI BstYI BstZ17I BtgZI
ClaI CspCI DraI DraIII EciI EcoRI EcoRV FalI
FseI FspI FspAI HincII HpaI KpnI MfeI MluI
NdeI NheI NruI NsiI NspI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI
Tth111I XbaI XhoI XmnI ZraI