Restriction map for
pF1KB9189
(Linear) (Six-base) MAPSORT of: KIBB9189.seq Check: 82 from: 1 to: 1287
KIBB9189.orf
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AccI GT'mk_AC
Cuts at: 0 103 1287
Size: 103 1184
AclI AA'CG_TT
Cuts at: 0 408 1287
Size: 408 879
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 301 933 940 1287
Size: 244 57 632 7 347
Fragments arranged by size:
632 347 244 57 7
AflII C'TTAA_G
Cuts at: 0 1066 1287
Size: 1066 221
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 166 198 1287
Size: 166 32 1089
Fragments arranged by size:
1089 166 32
ApaI G_GGCC'C
Cuts at: 0 1242 1287
Size: 1242 45
ApoI r'AATT_y
Cuts at: 0 193 583 985 1287
Size: 193 390 402 302
Fragments arranged by size:
402 390 302 193
BamHI G'GATC_C
Cuts at: 0 437 1287
Size: 437 850
BanII G_rGCy'C
Cuts at: 0 1242 1287
Size: 1242 45
BbsI GAAGACnn'nnnn_
Cuts at: 0 946 1151 1287
Size: 946 205 136
Fragments arranged by size:
946 205 136
Bme1580I G_kGCm'C
Cuts at: 0 1242 1287
Size: 1242 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 363 1287
Size: 363 924
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 1183 1287
Size: 95 1088 104
Fragments arranged by size:
1088 104 95
BsaI GGTCTCn'nnnn_
Cuts at: 0 12 308 1287
Size: 12 296 979
Fragments arranged by size:
979 296 12
BsaBI GATnn'nnATC
Cuts at: 0 829 1287
Size: 829 458
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1180 1183 1287
Size: 1180 3 104
Fragments arranged by size:
1180 104 3
BseYI C'CCAG_C
Cuts at: 0 207 1226 1241 1287
Size: 207 1019 15 46
Fragments arranged by size:
1019 207 46 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 799 1287
Size: 562 237 488
Fragments arranged by size:
562 488 237
Bsp1286I G_dGCh'C
Cuts at: 0 1242 1287
Size: 1242 45
BspHI T'CATG_A
Cuts at: 0 599 1287
Size: 599 688
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 392 771 1287
Size: 392 379 516
Fragments arranged by size:
516 392 379
BsrDI GCAATG_nn'
Cuts at: 0 276 560 624 798 1287
Size: 276 284 64 174 489
Fragments arranged by size:
489 284 276 174 64
BssSI C'ACGA_G
Cuts at: 0 432 1090 1287
Size: 432 658 197
Fragments arranged by size:
658 432 197
BstBI TT'CG_AA
Cuts at: 0 39 1287
Size: 39 1248
BstEII G'GTnAC_C
Cuts at: 0 498 1287
Size: 498 789
BstXI CCAn_nnnn'nTGG
Cuts at: 0 171 1234 1287
Size: 171 1063 53
Fragments arranged by size:
1063 171 53
BstYI r'GATC_y
Cuts at: 0 437 492 1287
Size: 437 55 795
Fragments arranged by size:
795 437 55
BtgI C'CryG_G
Cuts at: 0 5 11 302 1287
Size: 5 6 291 985
Fragments arranged by size:
985 291 6 5
BtsI GCAGTG_nn'
Cuts at: 0 710 1287
Size: 710 577
DrdI GACnn_nn'nnGTC
Cuts at: 0 91 1287
Size: 91 1196
EaeI y'GGCC_r
Cuts at: 0 2 8 57 1287
Size: 2 6 49 1230
Fragments arranged by size:
1230 49 6 2
EarI CTCTTCn'nnn_
Cuts at: 0 26 421 716 902 1287
Size: 26 395 295 186 385
Fragments arranged by size:
395 385 295 186 26
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 301 933 940 1287
Size: 244 57 632 7 347
Fragments arranged by size:
632 347 244 57 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 388 1287
Size: 388 899
EcoO109I rG'GnC_Cy
Cuts at: 0 1238 1287
Size: 1238 49
EcoRI G'AATT_C
Cuts at: 0 985 1287
Size: 985 302
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1081 1287
Size: 1081 206
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 675 707 1287
Size: 675 32 580
Fragments arranged by size:
675 580 32
HincII GTy'rAC
Cuts at: 0 96 287 297 1287
Size: 96 191 10 990
Fragments arranged by size:
990 191 96 10
HindIII A'AGCT_T
Cuts at: 0 901 1278 1287
Size: 901 377 9
Fragments arranged by size:
901 377 9
HpaI GTT'AAC
Cuts at: 0 287 1287
Size: 287 1000
MscI TGG'CCA
Cuts at: 0 59 1287
Size: 59 1228
MslI CAynn'nnrTG
Cuts at: 0 169 1287
Size: 169 1118
MspA1I CmG'CkG
Cuts at: 0 90 1201 1287
Size: 90 1111 86
Fragments arranged by size:
1111 90 86
NdeI CA'TA_TG
Cuts at: 0 966 1287
Size: 966 321
PasI CC'CwG_GG
Cuts at: 0 767 1287
Size: 767 520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 901 933 1287
Size: 901 32 354
Fragments arranged by size:
901 354 32
PspOMI G'GGCC_C
Cuts at: 0 1238 1287
Size: 1238 49
PstI C_TGCA'G
Cuts at: 0 1004 1287
Size: 1004 283
PvuII CAG'CTG
Cuts at: 0 90 1287
Size: 90 1197
SapI GCTCTTCn'nnn_
Cuts at: 0 26 1287
Size: 26 1261
SexAI A'CCwGG_T
Cuts at: 0 225 1287
Size: 225 1062
SfcI C'TryA_G
Cuts at: 0 1000 1213 1287
Size: 1000 213 74
Fragments arranged by size:
1000 213 74
SmlI C'TyrA_G
Cuts at: 0 110 1066 1162 1287
Size: 110 956 96 125
Fragments arranged by size:
956 125 110 96
StuI AGG'CCT
Cuts at: 0 379 1287
Size: 379 908
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 510 1287
Size: 510 777
XmnI GAAnn'nnTTC
Cuts at: 0 35 985 1180 1287
Size: 35 950 195 107
Fragments arranged by size:
950 195 107 35
Enzymes that do cut:
AccI AclI AcuI AflII AloI ApaI ApoI BamHI
BanII BbsI Bme1580I BmrI BpuEI BsaI BsaBI BseRI
BseYI BsgI Bsp1286I BspHI BspMI BsrDI BssSI BstBI
BstEII BstXI BstYI BtgI BtsI DrdI EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII HindIII
HpaI MscI MslI MspA1I NdeI PasI PpiI PspOMI
PstI PvuII SapI SexAI SfcI SmlI StuI XcmI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflIII AgeI AhdI AleI
AlfI AlwNI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BanI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BplI BpmI Bpu10I
BsaAI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BsrBI BsrFI BsrGI BssHII BstAPI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EciI
EcoICRI EcoRV FseI FspI FspAI HaeII KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PflMI PfoI
PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StyI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmaI ZraI