Restriction map for
pF1KB9185
(Linear) (Six-base) MAPSORT of: KIBB9185.seq Check: 3796 from: 1 to: 1128
KIBB9185.orf
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 50 617 1128
Size: 50 567 511
Fragments arranged by size:
567 511 50
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 678 951 1128
Size: 40 638 273 177
Fragments arranged by size:
638 273 177 40
AfeI AGC'GCT
Cuts at: 0 250 331 1128
Size: 250 81 797
Fragments arranged by size:
797 250 81
AflIII A'CryG_T
Cuts at: 0 503 755 1128
Size: 503 252 373
Fragments arranged by size:
503 373 252
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 663 1128
Size: 663 465
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 409 441 919 951 1128
Size: 409 32 478 32 177
Fragments arranged by size:
478 409 177 32 32
ApaLI G'TGCA_C
Cuts at: 0 99 1128
Size: 99 1029
AvaI C'yCGr_G
Cuts at: 0 496 1128
Size: 496 632
BamHI G'GATC_C
Cuts at: 0 725 1128
Size: 725 403
BanI G'GyrC_C
Cuts at: 0 479 1128
Size: 479 649
BanII G_rGCy'C
Cuts at: 0 352 1128
Size: 352 776
BbeI G_GCGC'C
Cuts at: 0 483 1128
Size: 483 645
BbsI GAAGACnn'nnnn_
Cuts at: 0 28 51 788 1128
Size: 28 23 737 340
Fragments arranged by size:
737 340 28 23
BbvCI CC'TCA_GC
Cuts at: 0 475 1128
Size: 475 653
Bme1580I G_kGCm'C
Cuts at: 0 103 389 1128
Size: 103 286 739
Fragments arranged by size:
739 286 103
BmgBI CAC'GTC
Cuts at: 0 404 1128
Size: 404 724
BmrI ACTGGGnnnn_n'
Cuts at: 0 21 1128
Size: 21 1107
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 791 1128
Size: 791 337
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 214 436 835 1077 1128
Size: 192 22 222 399 242 51
Fragments arranged by size:
399 242 222 192 51 22
Bpu10I CC'TnA_GC
Cuts at: 0 475 581 1128
Size: 475 106 547
Fragments arranged by size:
547 475 106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 1128
Size: 583 545
BsaI GGTCTCn'nnnn_
Cuts at: 0 538 1128
Size: 538 590
BsaAI yAC'GTr
Cuts at: 0 384 1128
Size: 384 744
BsaHI Gr'CG_yC
Cuts at: 0 480 983 1128
Size: 480 503 145
Fragments arranged by size:
503 480 145
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 187 217 409 439 1128
Size: 187 30 192 30 689
Fragments arranged by size:
689 192 187 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 460 780 1128
Size: 460 320 348
Fragments arranged by size:
460 348 320
BseYI C'CCAG_C
Cuts at: 0 342 720 1128
Size: 342 378 408
Fragments arranged by size:
408 378 342
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 192 1128
Size: 22 170 936
Fragments arranged by size:
936 170 22
BsiHKAI G_wGCw'C
Cuts at: 0 103 266 352 1128
Size: 103 163 86 776
Fragments arranged by size:
776 163 103 86
Bsp1286I G_dGCh'C
Cuts at: 0 103 266 352 389 1128
Size: 103 163 86 37 739
Fragments arranged by size:
739 163 103 86 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 50 617 1128
Size: 50 567 511
Fragments arranged by size:
567 511 50
BsrFI r'CCGG_y
Cuts at: 0 117 1128
Size: 117 1011
BsrGI T'GTAC_A
Cuts at: 0 217 1128
Size: 217 911
BstYI r'GATC_y
Cuts at: 0 725 1128
Size: 725 403
BtgI C'CryG_G
Cuts at: 0 239 461 616 913 1128
Size: 239 222 155 297 215
Fragments arranged by size:
297 239 222 215 155
DraIII CAC_nnn'GTG
Cuts at: 0 215 1128
Size: 215 913
DrdI GACnn_nn'nnGTC
Cuts at: 0 463 1128
Size: 463 665
EaeI y'GGCC_r
Cuts at: 0 115 1128
Size: 115 1013
EarI CTCTTCn'nnn_
Cuts at: 0 970 1128
Size: 970 158
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 766 1128
Size: 766 362
EcoICRI GAG'CTC
Cuts at: 0 350 1128
Size: 350 778
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 192 214 436 678 835 951 1077
Size: 40 152 22 222 242 157 116 126
Cuts at: 1077 1128
Size: 51
Fragments arranged by size:
242 222 157 152 126 116 51 40
22
EcoO109I rG'GnC_Cy
Cuts at: 0 357 1128
Size: 357 771
HaeII r_GCGC'y
Cuts at: 0 112 252 333 483 1128
Size: 112 140 81 150 645
Fragments arranged by size:
645 150 140 112 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 187 219 849 881 989 1021 1128
Size: 187 32 630 32 108 32 107
Fragments arranged by size:
630 187 108 107 32 32 32
KasI G'GCGC_C
Cuts at: 0 479 1128
Size: 479 649
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 1128
Size: 467 661
MslI CAynn'nnrTG
Cuts at: 0 568 760 1128
Size: 568 192 368
Fragments arranged by size:
568 368 192
MspA1I CmG'CkG
Cuts at: 0 96 618 670 792 995 1128
Size: 96 522 52 122 203 133
Fragments arranged by size:
522 203 133 122 96 52
NarI GG'CG_CC
Cuts at: 0 480 1128
Size: 480 648
NspI r_CATG'y
Cuts at: 0 507 759 879 1128
Size: 507 252 120 249
Fragments arranged by size:
507 252 249 120
PciI A'CATG_T
Cuts at: 0 503 755 1128
Size: 503 252 373
Fragments arranged by size:
503 373 252
PfoI T'CCnGG_A
Cuts at: 0 851 1128
Size: 851 277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 409 441 1128
Size: 409 32 687
Fragments arranged by size:
687 409 32
PpuMI rG'GwC_Cy
Cuts at: 0 357 1128
Size: 357 771
PvuII CAG'CTG
Cuts at: 0 96 670 1128
Size: 96 574 458
Fragments arranged by size:
574 458 96
SacI G_AGCT'C
Cuts at: 0 352 1128
Size: 352 776
SacII CC_GC'GG
Cuts at: 0 619 1128
Size: 619 509
SfoI GGC'GCC
Cuts at: 0 481 1128
Size: 481 647
SmlI C'TyrA_G
Cuts at: 0 598 1128
Size: 598 530
StyI C'CwwG_G
Cuts at: 0 353 623 1128
Size: 353 270 505
Fragments arranged by size:
505 353 270
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 302 1128
Size: 302 826
TatI w'GTAC_w
Cuts at: 0 217 500 518 1128
Size: 217 283 18 610
Fragments arranged by size:
610 283 217 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 309 341 1128
Size: 309 32 787
Fragments arranged by size:
787 309 32
Enzymes that do cut:
AarI AcuI AfeI AflIII AlfI AloI ApaLI AvaI
BamHI BanI BanII BbeI BbsI BbvCI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI
BsrGI BstYI BtgI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI MmeI MslI
MspA1I NarI NspI PciI PfoI PpiI PpuMI PvuII
SacI SacII SfoI SmlI StyI TaqII TatI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlwNI ApaI ApoI AscI AseI AsiSI AvrII BaeI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmtI
BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI EagI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MscI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI