Restriction map for
pF1KB8991
(Linear) (Six-base) MAPSORT of: KIBB8991.seq Check: 7499 from: 1 to: 465
KIBB8991.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 64 96 465
Size: 64 32 369
Fragments arranged by size:
369 64 32
ApaI G_GGCC'C
Cuts at: 0 7 465
Size: 7 458
ApoI r'AATT_y
Cuts at: 0 35 465
Size: 35 430
AvaI C'yCGr_G
Cuts at: 0 7 225 465
Size: 7 218 240
Fragments arranged by size:
240 218 7
BanII G_rGCy'C
Cuts at: 0 7 50 465
Size: 7 43 415
Fragments arranged by size:
415 43 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 216 250 465
Size: 216 34 215
Fragments arranged by size:
216 215 34
BfrBI ATG'CAT
Cuts at: 0 333 465
Size: 333 132
BglI GCCn_nnn'nGGC
Cuts at: 0 301 465
Size: 301 164
Bme1580I G_kGCm'C
Cuts at: 0 7 465
Size: 7 458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 187 465
Size: 34 153 278
Fragments arranged by size:
278 153 34
BsaWI w'CCGG_w
Cuts at: 0 459 465
Size: 459 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 64 94 465
Size: 64 30 371
Fragments arranged by size:
371 64 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 154 283 465
Size: 154 129 182
Fragments arranged by size:
182 154 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 256 406 465
Size: 232 24 150 59
Fragments arranged by size:
232 150 59 24
BsiHKAI G_wGCw'C
Cuts at: 0 127 465
Size: 127 338
Bsp1286I G_dGCh'C
Cuts at: 0 7 50 127 465
Size: 7 43 77 338
Fragments arranged by size:
338 77 43 7
BspEI T'CCGG_A
Cuts at: 0 459 465
Size: 459 6
BsrBI CCG'CTC
Cuts at: 0 70 465
Size: 70 395
BssHII G'CGCG_C
Cuts at: 0 192 216 465
Size: 192 24 249
Fragments arranged by size:
249 192 24
EaeI y'GGCC_r
Cuts at: 0 302 465
Size: 302 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 7 331 465
Size: 7 324 134
Fragments arranged by size:
324 134 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 187 465
Size: 34 153 278
Fragments arranged by size:
278 153 34
EcoO109I rG'GnC_Cy
Cuts at: 0 4 144 171 465
Size: 4 140 27 294
Fragments arranged by size:
294 140 27 4
MfeI C'AATT_G
Cuts at: 0 391 465
Size: 391 74
MscI TGG'CCA
Cuts at: 0 304 465
Size: 304 161
MslI CAynn'nnrTG
Cuts at: 0 382 465
Size: 382 83
MspA1I CmG'CkG
Cuts at: 0 23 162 351 465
Size: 23 139 189 114
Fragments arranged by size:
189 139 114 23
NsiI A_TGCA'T
Cuts at: 0 335 465
Size: 335 130
PasI CC'CwG_GG
Cuts at: 0 42 465
Size: 42 423
PflMI CCAn_nnn'nTGG
Cuts at: 0 351 465
Size: 351 114
PfoI T'CCnGG_A
Cuts at: 0 134 465
Size: 134 331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 64 96 465
Size: 64 32 369
Fragments arranged by size:
369 64 32
PpuMI rG'GwC_Cy
Cuts at: 0 171 465
Size: 171 294
PspOMI G'GGCC_C
Cuts at: 0 3 465
Size: 3 462
PstI C_TGCA'G
Cuts at: 0 28 465
Size: 28 437
PvuII CAG'CTG
Cuts at: 0 351 465
Size: 351 114
SfcI C'TryA_G
Cuts at: 0 24 465
Size: 24 441
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 301 465
Size: 301 164
SmaI CCC'GGG
Cuts at: 0 9 465
Size: 9 456
SmlI C'TyrA_G
Cuts at: 0 225 465
Size: 225 240
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 162 465
Size: 162 303
XhoI C'TCGA_G
Cuts at: 0 225 465
Size: 225 240
XmaI C'CCGG_G
Cuts at: 0 7 465
Size: 7 458
Enzymes that do cut:
AloI ApaI ApoI AvaI BanII BcgI BfrBI BglI
Bme1580I BpmI BsaWI BsaXI BseRI BsgI BsiHKAI Bsp1286I
BspEI BsrBI BssHII EaeI EciI Eco57MI EcoO109I MfeI
MscI MslI MspA1I NsiI PasI PflMI PfoI PpiI
PpuMI PspOMI PstI PvuII SfcI SfiI SmaI SmlI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BciVI BclI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BseYI
BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EarI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI PacI PciI PmeI
PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XmnI
ZraI