Restriction map for
pF1KB8977
(Linear) (Six-base) MAPSORT of: KIBB8977.seq Check: 7322 from: 1 to: 1119
KIBB8977.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
Acc65I G'GTAC_C
Cuts at: 0 767 1119
Size: 767 352
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 1061 1119
Size: 38 1023 58
Fragments arranged by size:
1023 58 38
AfeI AGC'GCT
Cuts at: 0 794 1119
Size: 794 325
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1079 1119
Size: 1079 40
AlwNI CAG_nnn'CTG
Cuts at: 0 117 220 493 595 631 1119
Size: 117 103 273 102 36 488
Fragments arranged by size:
488 273 117 103 102 36
ApaI G_GGCC'C
Cuts at: 0 933 1119
Size: 933 186
AvaI C'yCGr_G
Cuts at: 0 1091 1119
Size: 1091 28
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 31 64 1119
Size: 31 33 1055
Fragments arranged by size:
1055 33 31
BanI G'GyrC_C
Cuts at: 0 767 1119
Size: 767 352
BanII G_rGCy'C
Cuts at: 0 149 254 620 933 1119
Size: 149 105 366 313 186
Fragments arranged by size:
366 313 186 149 105
BbsI GAAGACnn'nnnn_
Cuts at: 0 425 1119
Size: 425 694
BglI GCCn_nnn'nGGC
Cuts at: 0 338 399 1119
Size: 338 61 720
Fragments arranged by size:
720 338 61
BlpI GC'TnA_GC
Cuts at: 0 641 1119
Size: 641 478
Bme1580I G_kGCm'C
Cuts at: 0 933 1091 1119
Size: 933 158 28
Fragments arranged by size:
933 158 28
BmrI ACTGGGnnnn_n'
Cuts at: 0 523 1119
Size: 523 596
BmtI G_CTAG'C
Cuts at: 0 793 1119
Size: 793 326
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 639 1119
Size: 639 480
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 901 913 976 1119
Size: 499 402 12 63 143
Fragments arranged by size:
499 402 143 63 12
Bpu10I CC'TnA_GC
Cuts at: 0 369 407 1119
Size: 369 38 712
Fragments arranged by size:
712 369 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 1119
Size: 493 626
BsaI GGTCTCn'nnnn_
Cuts at: 0 592 1119
Size: 592 527
BsaWI w'CCGG_w
Cuts at: 0 947 1119
Size: 947 172
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 79 109 1119
Size: 79 30 1010
Fragments arranged by size:
1010 79 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 612 1119
Size: 612 507
BseYI C'CCAG_C
Cuts at: 0 17 156 255 1119
Size: 17 139 99 864
Fragments arranged by size:
864 139 99 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 635 895 1119
Size: 635 260 224
Fragments arranged by size:
635 260 224
BsiHKAI G_wGCw'C
Cuts at: 0 254 1119
Size: 254 865
BsmI GAATG_Cn'
Cuts at: 0 126 1119
Size: 126 993
Bsp1286I G_dGCh'C
Cuts at: 0 149 254 620 933 1091 1119
Size: 149 105 366 313 158 28
Fragments arranged by size:
366 313 158 149 105 28
BsrBI CCG'CTC
Cuts at: 0 357 885 897 918 1119
Size: 357 528 12 21 201
Fragments arranged by size:
528 357 201 21 12
BsrFI r'CCGG_y
Cuts at: 0 735 1119
Size: 735 384
BstXI CCAn_nnnn'nTGG
Cuts at: 0 564 1119
Size: 564 555
BstYI r'GATC_y
Cuts at: 0 279 1119
Size: 279 840
Bsu36I CC'TnA_GG
Cuts at: 0 624 1119
Size: 624 495
BtgI C'CryG_G
Cuts at: 0 933 1119
Size: 933 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 473 1119
Size: 473 646
BtsI GCAGTG_nn'
Cuts at: 0 647 1119
Size: 647 472
DrdI GACnn_nn'nnGTC
Cuts at: 0 724 1119
Size: 724 395
EaeI y'GGCC_r
Cuts at: 0 305 692 1119
Size: 305 387 427
Fragments arranged by size:
427 387 305
EarI CTCTTCn'nnn_
Cuts at: 0 1037 1119
Size: 1037 82
EcoICRI GAG'CTC
Cuts at: 0 252 1119
Size: 252 867
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 499 901 913 976 1061 1119
Size: 38 461 402 12 63 85 58
Fragments arranged by size:
461 402 85 63 58 38 12
EcoO109I rG'GnC_Cy
Cuts at: 0 628 929 1119
Size: 628 301 190
Fragments arranged by size:
628 301 190
HaeII r_GCGC'y
Cuts at: 0 796 1119
Size: 796 323
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 79 111 412 431 444 451 463 483
Size: 79 32 301 19 13 7 12 20
Cuts at: 483 1119
Size: 636
Fragments arranged by size:
636 301 79 32 20 19 13 12
7
HincII GTy'rAC
Cuts at: 0 729 1119
Size: 729 390
KpnI G_GTAC'C
Cuts at: 0 771 1119
Size: 771 348
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 599 834 1119
Size: 455 144 235 285
Fragments arranged by size:
455 285 235 144
MscI TGG'CCA
Cuts at: 0 307 694 1119
Size: 307 387 425
Fragments arranged by size:
425 387 307
MspA1I CmG'CkG
Cuts at: 0 45 592 774 879 935 975 1119
Size: 45 547 182 105 56 40 144
Fragments arranged by size:
547 182 144 105 56 45 40
NheI G'CTAG_C
Cuts at: 0 789 1119
Size: 789 330
PflMI CCAn_nnn'nTGG
Cuts at: 0 169 220 1119
Size: 169 51 899
Fragments arranged by size:
899 169 51
PfoI T'CCnGG_A
Cuts at: 0 377 800 1119
Size: 377 423 319
Fragments arranged by size:
423 377 319
PspOMI G'GGCC_C
Cuts at: 0 929 1119
Size: 929 190
PstI C_TGCA'G
Cuts at: 0 113 1119
Size: 113 1006
PvuII CAG'CTG
Cuts at: 0 45 592 879 975 1119
Size: 45 547 287 96 144
Fragments arranged by size:
547 287 144 96 45
SacI G_AGCT'C
Cuts at: 0 254 1119
Size: 254 865
SacII CC_GC'GG
Cuts at: 0 936 1119
Size: 936 183
SfcI C'TryA_G
Cuts at: 0 109 453 1119
Size: 109 344 666
Fragments arranged by size:
666 344 109
SmaI CCC'GGG
Cuts at: 0 1093 1119
Size: 1093 26
SmlI C'TyrA_G
Cuts at: 0 472 1119
Size: 472 647
StuI AGG'CCT
Cuts at: 0 341 1119
Size: 341 778
StyI C'CwwG_G
Cuts at: 0 168 563 605 689 1119
Size: 168 395 42 84 430
Fragments arranged by size:
430 395 168 84 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 198 227 1119
Size: 198 29 892
Fragments arranged by size:
892 198 29
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 217 876 1119
Size: 217 659 243
Fragments arranged by size:
659 243 217
XmaI C'CCGG_G
Cuts at: 0 1091 1119
Size: 1091 28
Enzymes that do cut:
Acc65I AcuI AfeI AlfI AlwNI ApaI AvaI BaeI
BanI BanII BbsI BglI BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BsrBI BsrFI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EarI
EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII KpnI MmeI
MscI MspA1I NheI PflMI PfoI PspOMI PstI PvuII
SacI SacII SfcI SmaI SmlI StuI StyI TaqII
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BmgBI BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BspEI
BspHI BspMI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I ClaI CspCI DraI DraIII EagI EciI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KasI MfeI MluI MslI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TatI TstI Tth111I XbaI XhoI
XmnI ZraI