Restriction map for
pF1KB8950
(Linear) (Six-base) MAPSORT of: KIBB8950.seq Check: 1199 from: 1 to: 972
KIBB8950.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
Acc65I G'GTAC_C
Cuts at: 0 482 972
Size: 482 490
AlwNI CAG_nnn'CTG
Cuts at: 0 603 972
Size: 603 369
ApaI G_GGCC'C
Cuts at: 0 176 972
Size: 176 796
AvaI C'yCGr_G
Cuts at: 0 175 398 916 936 972
Size: 175 223 518 20 36
Fragments arranged by size:
518 223 175 36 20
AvrII C'CTAG_G
Cuts at: 0 690 972
Size: 690 282
BanI G'GyrC_C
Cuts at: 0 123 482 657 702 972
Size: 123 359 175 45 270
Fragments arranged by size:
359 270 175 123 45
BanII G_rGCy'C
Cuts at: 0 98 176 916 972
Size: 98 78 740 56
Fragments arranged by size:
740 98 78 56
BbeI G_GCGC'C
Cuts at: 0 127 706 972
Size: 127 579 266
Fragments arranged by size:
579 266 127
BbsI GAAGACnn'nnnn_
Cuts at: 0 376 972
Size: 376 596
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 166 200 972
Size: 166 34 772
Fragments arranged by size:
772 166 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 970 972
Size: 970 2
BglII A'GATC_T
Cuts at: 0 548 972
Size: 548 424
Bme1580I G_kGCm'C
Cuts at: 0 176 972
Size: 176 796
BmgBI CAC'GTC
Cuts at: 0 533 972
Size: 533 439
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 385 478 625 972
Size: 130 255 93 147 347
Fragments arranged by size:
347 255 147 130 93
BsaAI yAC'GTr
Cuts at: 0 283 972
Size: 283 689
BsaHI Gr'CG_yC
Cuts at: 0 124 703 887 972
Size: 124 579 184 85
Fragments arranged by size:
579 184 124 85
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 120 972
Size: 120 852
BseYI C'CCAG_C
Cuts at: 0 7 169 216 303 837 972
Size: 7 162 47 87 534 135
Fragments arranged by size:
534 162 135 87 47 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 792 972
Size: 792 180
BsiHKAI G_wGCw'C
Cuts at: 0 98 431 972
Size: 98 333 541
Fragments arranged by size:
541 333 98
BsmI GAATG_Cn'
Cuts at: 0 906 972
Size: 906 66
Bsp1286I G_dGCh'C
Cuts at: 0 98 176 431 916 972
Size: 98 78 255 485 56
Fragments arranged by size:
485 255 98 78 56
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 435 531 972
Size: 435 96 441
Fragments arranged by size:
441 435 96
BsrBI CCG'CTC
Cuts at: 0 393 462 972
Size: 393 69 510
Fragments arranged by size:
510 393 69
BsrFI r'CCGG_y
Cuts at: 0 234 563 846 972
Size: 234 329 283 126
Fragments arranged by size:
329 283 234 126
BssHII G'CGCG_C
Cuts at: 0 207 224 341 972
Size: 207 17 117 631
Fragments arranged by size:
631 207 117 17
BstAPI GCAn_nnn'nTGC
Cuts at: 0 686 972
Size: 686 286
BstYI r'GATC_y
Cuts at: 0 548 972
Size: 548 424
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 689 972
Size: 689 283
BtsI GCAGTG_nn'
Cuts at: 0 809 851 972
Size: 809 42 121
Fragments arranged by size:
809 121 42
EaeI y'GGCC_r
Cuts at: 0 143 236 512 848 972
Size: 143 93 276 336 124
Fragments arranged by size:
336 276 143 124 93
EarI CTCTTCn'nnn_
Cuts at: 0 436 972
Size: 436 536
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 394 426 972
Size: 394 32 546
Fragments arranged by size:
546 394 32
EcoICRI GAG'CTC
Cuts at: 0 96 972
Size: 96 876
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 385 478 625 972
Size: 130 255 93 147 347
Fragments arranged by size:
347 255 147 130 93
EcoO109I rG'GnC_Cy
Cuts at: 0 312 972
Size: 312 660
HaeII r_GCGC'y
Cuts at: 0 127 468 570 706 972
Size: 127 341 102 136 266
Fragments arranged by size:
341 266 136 127 102
KasI G'GCGC_C
Cuts at: 0 123 702 972
Size: 123 579 270
Fragments arranged by size:
579 270 123
KpnI G_GTAC'C
Cuts at: 0 486 972
Size: 486 486
MscI TGG'CCA
Cuts at: 0 514 972
Size: 514 458
MspA1I CmG'CkG
Cuts at: 0 480 510 588 806 972
Size: 480 30 78 218 166
Fragments arranged by size:
480 218 166 78 30
NaeI GCC'GGC
Cuts at: 0 848 972
Size: 848 124
NarI GG'CG_CC
Cuts at: 0 124 703 972
Size: 124 579 269
Fragments arranged by size:
579 269 124
NgoMIV G'CCGG_C
Cuts at: 0 846 972
Size: 846 126
NspI r_CATG'y
Cuts at: 0 141 972
Size: 141 831
PflMI CCAn_nnn'nTGG
Cuts at: 0 603 972
Size: 603 369
PmlI CAC'GTG
Cuts at: 0 283 972
Size: 283 689
PpuMI rG'GwC_Cy
Cuts at: 0 312 972
Size: 312 660
PsiI TTA'TAA
Cuts at: 0 749 972
Size: 749 223
PspOMI G'GGCC_C
Cuts at: 0 172 972
Size: 172 800
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 553 585 972
Size: 553 32 387
Fragments arranged by size:
553 387 32
PstI C_TGCA'G
Cuts at: 0 350 793 972
Size: 350 443 179
Fragments arranged by size:
443 350 179
PvuII CAG'CTG
Cuts at: 0 510 806 972
Size: 510 296 166
Fragments arranged by size:
510 296 166
SacI G_AGCT'C
Cuts at: 0 98 972
Size: 98 874
SapI GCTCTTCn'nnn_
Cuts at: 0 436 972
Size: 436 536
SfcI C'TryA_G
Cuts at: 0 288 346 789 801 972
Size: 288 58 443 12 171
Fragments arranged by size:
443 288 171 58 12
SfoI GGC'GCC
Cuts at: 0 125 704 972
Size: 125 579 268
Fragments arranged by size:
579 268 125
SmaI CCC'GGG
Cuts at: 0 400 918 972
Size: 400 518 54
Fragments arranged by size:
518 400 54
SphI G_CATG'C
Cuts at: 0 141 972
Size: 141 831
StuI AGG'CCT
Cuts at: 0 163 972
Size: 163 809
StyI C'CwwG_G
Cuts at: 0 308 690 972
Size: 308 382 282
Fragments arranged by size:
382 308 282
XmaI C'CCGG_G
Cuts at: 0 398 916 972
Size: 398 518 56
Fragments arranged by size:
518 398 56
Enzymes that do cut:
Acc65I AlwNI ApaI AvaI AvrII BanI BanII BbeI
BbsI BcgI BciVI BglII Bme1580I BmgBI BpmI BsaAI
BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrFI BssHII BstAPI BstYI BtgZI BtsI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I HaeII KasI KpnI
MscI MspA1I NaeI NarI NgoMIV NspI PflMI PmlI
PpuMI PsiI PspOMI PsrI PstI PvuII SacI SapI
SfcI SfoI SmaI SphI StuI StyI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI BaeI BamHI BbvCI BclI BfrBI BglI
BlpI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI
BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspHI BsrDI
BsrGI BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraI DraIII DrdI EagI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI MfeI MluI MmeI MslI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PpiI PshAI PspXI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI
SpeI SrfI SspI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmnI ZraI