Restriction map for
pF1KB8936
(Linear) (Six-base) MAPSORT of: KIBB8936.seq Check: 5774 from: 1 to: 855
KIBB8936.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 827 855
Size: 827 28
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 855
Size: 147 708
AflII C'TTAA_G
Cuts at: 0 832 855
Size: 832 23
AflIII A'CryG_T
Cuts at: 0 132 692 855
Size: 132 560 163
Fragments arranged by size:
560 163 132
AlwNI CAG_nnn'CTG
Cuts at: 0 759 855
Size: 759 96
ApaI G_GGCC'C
Cuts at: 0 643 855
Size: 643 212
ApaLI G'TGCA_C
Cuts at: 0 32 85 790 855
Size: 32 53 705 65
Fragments arranged by size:
705 65 53 32
AvaI C'yCGr_G
Cuts at: 0 46 183 289 368 464 642 650 730
Size: 46 137 106 79 96 178 8 80
Cuts at: 730 855
Size: 125
Fragments arranged by size:
178 137 125 106 96 80 79 46
8
BanI G'GyrC_C
Cuts at: 0 91 855
Size: 91 764
BanII G_rGCy'C
Cuts at: 0 20 62 500 643 815 855
Size: 20 42 438 143 172 40
Fragments arranged by size:
438 172 143 42 40 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 186 327 855
Size: 186 141 528
Fragments arranged by size:
528 186 141
BglI GCCn_nnn'nGGC
Cuts at: 0 277 479 596 855
Size: 277 202 117 259
Fragments arranged by size:
277 259 202 117
Bme1580I G_kGCm'C
Cuts at: 0 36 89 368 643 794 855
Size: 36 53 279 275 151 61
Fragments arranged by size:
279 275 151 61 53 36
BmgBI CAC'GTC
Cuts at: 0 693 855
Size: 693 162
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 226 412 855
Size: 226 186 443
Fragments arranged by size:
443 226 186
BseYI C'CCAG_C
Cuts at: 0 222 403 855
Size: 222 181 452
Fragments arranged by size:
452 222 181
BsiEI CG_ry'CG
Cuts at: 0 551 605 855
Size: 551 54 250
Fragments arranged by size:
551 250 54
BsiHKAI G_wGCw'C
Cuts at: 0 36 89 794 855
Size: 36 53 705 61
Fragments arranged by size:
705 61 53 36
BsmI GAATG_Cn'
Cuts at: 0 458 855
Size: 458 397
BsmBI CGTCTCn'nnnn_
Cuts at: 0 250 855
Size: 250 605
Bsp1286I G_dGCh'C
Cuts at: 0 20 36 62 89 368 500 643 794
Size: 20 16 26 27 279 132 143 151
Cuts at: 794 815 855
Size: 21 40
Fragments arranged by size:
279 151 143 132 40 27 26 21
20 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 827 855
Size: 827 28
BsrFI r'CCGG_y
Cuts at: 0 488 794 855
Size: 488 306 61
Fragments arranged by size:
488 306 61
BssHII G'CGCG_C
Cuts at: 0 553 855
Size: 553 302
BssSI C'ACGA_G
Cuts at: 0 424 855
Size: 424 431
BtgI C'CryG_G
Cuts at: 0 20 150 855
Size: 20 130 705
Fragments arranged by size:
705 130 20
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 117 152 855
Size: 117 35 703
Fragments arranged by size:
703 117 35
DraIII CAC_nnn'GTG
Cuts at: 0 156 855
Size: 156 699
DrdI GACnn_nn'nnGTC
Cuts at: 0 242 855
Size: 242 613
EaeI y'GGCC_r
Cuts at: 0 490 602 800 855
Size: 490 112 198 55
Fragments arranged by size:
490 198 112 55
EagI C'GGCC_G
Cuts at: 0 602 855
Size: 602 253
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 403 855
Size: 403 452
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 855
Size: 147 708
FspI TGC'GCA
Cuts at: 0 442 668 855
Size: 442 226 187
Fragments arranged by size:
442 226 187
HaeII r_GCGC'y
Cuts at: 0 54 650 855
Size: 54 596 205
Fragments arranged by size:
596 205 54
HincII GTy'rAC
Cuts at: 0 315 855
Size: 315 540
MluI A'CGCG_T
Cuts at: 0 132 855
Size: 132 723
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 571 855
Size: 439 132 284
Fragments arranged by size:
439 284 132
MscI TGG'CCA
Cuts at: 0 802 855
Size: 802 53
MslI CAynn'nnrTG
Cuts at: 0 103 855
Size: 103 752
MspA1I CmG'CkG
Cuts at: 0 177 397 664 855
Size: 177 220 267 191
Fragments arranged by size:
267 220 191 177
NaeI GCC'GGC
Cuts at: 0 490 855
Size: 490 365
NgoMIV G'CCGG_C
Cuts at: 0 488 855
Size: 488 367
NspI r_CATG'y
Cuts at: 0 612 855
Size: 612 243
PflMI CCAn_nnn'nTGG
Cuts at: 0 513 855
Size: 513 342
PspOMI G'GGCC_C
Cuts at: 0 639 855
Size: 639 216
PspXI vC'TCGA_Gb
Cuts at: 0 46 730 855
Size: 46 684 125
Fragments arranged by size:
684 125 46
PstI C_TGCA'G
Cuts at: 0 755 855
Size: 755 100
PvuII CAG'CTG
Cuts at: 0 664 855
Size: 664 191
SfcI C'TryA_G
Cuts at: 0 501 751 855
Size: 501 250 104
Fragments arranged by size:
501 250 104
SmaI CCC'GGG
Cuts at: 0 185 370 466 652 855
Size: 185 185 96 186 203
Fragments arranged by size:
203 186 185 185 96
SmlI C'TyrA_G
Cuts at: 0 46 730 832 855
Size: 46 684 102 23
Fragments arranged by size:
684 102 46 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 238 559 855
Size: 238 321 296
Fragments arranged by size:
321 296 238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 102 733 855
Size: 102 631 122
Fragments arranged by size:
631 122 102
XhoI C'TCGA_G
Cuts at: 0 46 730 855
Size: 46 684 125
Fragments arranged by size:
684 125 46
XmaI C'CCGG_G
Cuts at: 0 183 368 464 650 855
Size: 183 185 96 186 205
Fragments arranged by size:
205 186 185 183 96
Enzymes that do cut:
AarI AcuI AflII AflIII AlwNI ApaI ApaLI AvaI
BanI BanII BbsI BglI Bme1580I BmgBI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrFI BssHII
BssSI BtgI CspCI DraIII DrdI EaeI EagI EciI
Eco57MI FspI HaeII HincII MluI MmeI MscI MslI
MspA1I NaeI NgoMIV NspI PflMI PspOMI PspXI PstI
PvuII SfcI SmaI SmlI TaqII XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiWI BspEI
BspHI BsrBI BsrDI BsrGI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI BtsI ClaI DraI EarI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspAI
Hin4I HindIII HpaI KasI KpnI MfeI NarI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TatI TstI
Tth111I XbaI XmnI ZraI