(Linear) (Six-base) MAPSORT of: KIBB8896.seq  Check: 4322  from: 1  to: 669



KIBB8896.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

Acc65I G'GTAC_C

Cuts at:      0     580     669 
   Size:        580      89
 
AflIII A'CryG_T

Cuts at:      0     530     669 
   Size:        530     139
 
AleI CACnn'nnGTG

Cuts at:      0     338     669 
   Size:        338     331
 
AlwNI CAG_nnn'CTG

Cuts at:      0     618     669 
   Size:        618      51
 
ApaLI G'TGCA_C

Cuts at:      0     331     669 
   Size:        331     338
 
ApoI r'AATT_y

Cuts at:      0     373     669 
   Size:        373     296
 
BanI G'GyrC_C

Cuts at:      0     580     669 
   Size:        580      89
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     291     669 
   Size:        291     378
 
BclI T'GATC_A

Cuts at:      0     108     122     669 
   Size:        108      14     547

  Fragments arranged by size: 

                 547     108      14
 
BlpI GC'TnA_GC

Cuts at:      0     437     669 
   Size:        437     232
 
Bme1580I G_kGCm'C

Cuts at:      0     335     669 
   Size:        335     334
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     311     669 
   Size:        154     157     358

  Fragments arranged by size: 

                 358     157     154
 
BseYI C'CCAG_C

Cuts at:      0     347     669 
   Size:        347     322
 
BsiHKAI G_wGCw'C

Cuts at:      0     335     669 
   Size:        335     334
 
Bsp1286I G_dGCh'C

Cuts at:      0     335     669 
   Size:        335     334
 
BsrGI T'GTAC_A

Cuts at:      0     527     669 
   Size:        527     142
 
BssSI C'ACGA_G

Cuts at:      0     188     669 
   Size:        188     481
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      42     603     669 
   Size:         42     561      66

  Fragments arranged by size: 

                 561      66      42
 
BstYI r'GATC_y

Cuts at:      0      20     669 
   Size:         20     649
 
EarI CTCTTCn'nnn_

Cuts at:      0     208     669 
   Size:        208     461
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     311     669 
   Size:        154     157     358

  Fragments arranged by size: 

                 358     157     154
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     457     669 
   Size:        457     212
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     343     375     626     629     658     661     669 
   Size:        343      32     251       3      29       3       8

  Fragments arranged by size: 

                 343     251      32      29       8       3       3
 
KpnI G_GTAC'C

Cuts at:      0     584     669 
   Size:        584      85
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     240     669 
   Size:        240     429
 
MslI CAynn'nnrTG

Cuts at:      0     338     340     669 
   Size:        338       2     329

  Fragments arranged by size: 

                 338     329       2
 
MspA1I CmG'CkG

Cuts at:      0      12     132     669 
   Size:         12     120     537

  Fragments arranged by size: 

                 537     120      12
 
NspI r_CATG'y

Cuts at:      0     534     669 
   Size:        534     135
 
PciI A'CATG_T

Cuts at:      0     530     669 
   Size:        530     139
 
PfoI T'CCnGG_A

Cuts at:      0      41     602     669 
   Size:         41     561      67

  Fragments arranged by size: 

                 561      67      41
 
PsiI TTA'TAA

Cuts at:      0     248     669 
   Size:        248     421
 
SfcI C'TryA_G

Cuts at:      0     145     174     195     669 
   Size:        145      29      21     474

  Fragments arranged by size: 

                 474     145      29      21
 
SpeI A'CTAG_T

Cuts at:      0     240     669 
   Size:        240     429
 
StyI C'CwwG_G

Cuts at:      0     219     656     669 
   Size:        219     437      13

  Fragments arranged by size: 

                 437     219      13
 
TatI w'GTAC_w

Cuts at:      0     527     669 
   Size:        527     142
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     450     669 
   Size:        450     219
 

 Enzymes that do cut:

   Acc65I   AflIII     AleI    AlwNI    ApaLI     ApoI     BanI     BbsI
     BclI     BlpI Bme1580I     BpmI    BseYI  BsiHKAI Bsp1286I    BsrGI
    BssSI    BstXI    BstYI     EarI  Eco57MI    EcoNI    Hin4I     KpnI
     MmeI     MslI   MspA1I     NspI     PciI     PfoI     PsiI     SfcI
     SpeI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI     ApaI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BglII    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     MfeI     MluI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI