Restriction map for
pF1KB8889
(Linear) (Six-base) MAPSORT of: KIBB8889.seq Check: 9785 from: 1 to: 612
KIBB8889.orf
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AatII G_ACGT'C
Cuts at: 0 401 612
Size: 401 211
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 492 612
Size: 492 120
AgeI A'CCGG_T
Cuts at: 0 179 612
Size: 179 433
AlwNI CAG_nnn'CTG
Cuts at: 0 420 612
Size: 420 192
AvaI C'yCGr_G
Cuts at: 0 92 109 130 211 404 612
Size: 92 17 21 81 193 208
Fragments arranged by size:
208 193 92 81 21 17
BanI G'GyrC_C
Cuts at: 0 103 117 612
Size: 103 14 495
Fragments arranged by size:
495 103 14
BanII G_rGCy'C
Cuts at: 0 439 612
Size: 439 173
BbeI G_GCGC'C
Cuts at: 0 121 612
Size: 121 491
BlpI GC'TnA_GC
Cuts at: 0 433 612
Size: 433 179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 612
Size: 343 269
Bpu10I CC'TnA_GC
Cuts at: 0 476 612
Size: 476 136
BsaHI Gr'CG_yC
Cuts at: 0 118 398 612
Size: 118 280 214
Fragments arranged by size:
280 214 118
BsaWI w'CCGG_w
Cuts at: 0 179 612
Size: 179 433
BseYI C'CCAG_C
Cuts at: 0 485 612
Size: 485 127
BsiEI CG_ry'CG
Cuts at: 0 158 612
Size: 158 454
BsiHKAI G_wGCw'C
Cuts at: 0 257 439 612
Size: 257 182 173
Fragments arranged by size:
257 182 173
Bsp1286I G_dGCh'C
Cuts at: 0 257 439 612
Size: 257 182 173
Fragments arranged by size:
257 182 173
BsrBI CCG'CTC
Cuts at: 0 60 612
Size: 60 552
BsrFI r'CCGG_y
Cuts at: 0 114 179 612
Size: 114 65 433
Fragments arranged by size:
433 114 65
BssSI C'ACGA_G
Cuts at: 0 298 612
Size: 298 314
BstEII G'GTnAC_C
Cuts at: 0 339 612
Size: 339 273
BtgI C'CryG_G
Cuts at: 0 29 152 373 612
Size: 29 123 221 239
Fragments arranged by size:
239 221 123 29
EaeI y'GGCC_r
Cuts at: 0 32 68 155 612
Size: 32 36 87 457
Fragments arranged by size:
457 87 36 32
EagI C'GGCC_G
Cuts at: 0 155 612
Size: 155 457
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 183 259 612
Size: 183 76 353
Fragments arranged by size:
353 183 76
EcoICRI GAG'CTC
Cuts at: 0 437 612
Size: 437 175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 492 612
Size: 343 149 120
Fragments arranged by size:
343 149 120
EcoO109I rG'GnC_Cy
Cuts at: 0 512 612
Size: 512 100
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 381 612
Size: 381 231
HaeII r_GCGC'y
Cuts at: 0 121 238 420 612
Size: 121 117 182 192
Fragments arranged by size:
192 182 121 117
HincII GTy'rAC
Cuts at: 0 6 575 612
Size: 6 569 37
Fragments arranged by size:
569 37 6
HpaI GTT'AAC
Cuts at: 0 575 612
Size: 575 37
KasI G'GCGC_C
Cuts at: 0 117 612
Size: 117 495
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 612
Size: 284 328
MslI CAynn'nnrTG
Cuts at: 0 28 612
Size: 28 584
MspA1I CmG'CkG
Cuts at: 0 42 154 336 375 546 612
Size: 42 112 182 39 171 66
Fragments arranged by size:
182 171 112 66 42 39
NaeI GCC'GGC
Cuts at: 0 116 612
Size: 116 496
NarI GG'CG_CC
Cuts at: 0 118 612
Size: 118 494
NcoI C'CATG_G
Cuts at: 0 29 612
Size: 29 583
NgoMIV G'CCGG_C
Cuts at: 0 114 612
Size: 114 498
NotI GC'GGCC_GC
Cuts at: 0 155 612
Size: 155 457
PflMI CCAn_nnn'nTGG
Cuts at: 0 420 612
Size: 420 192
PpuMI rG'GwC_Cy
Cuts at: 0 512 612
Size: 512 100
PstI C_TGCA'G
Cuts at: 0 473 612
Size: 473 139
PvuII CAG'CTG
Cuts at: 0 546 612
Size: 546 66
SacI G_AGCT'C
Cuts at: 0 439 612
Size: 439 173
SacII CC_GC'GG
Cuts at: 0 155 376 612
Size: 155 221 236
Fragments arranged by size:
236 221 155
SfcI C'TryA_G
Cuts at: 0 469 612
Size: 469 143
SfoI GGC'GCC
Cuts at: 0 119 612
Size: 119 493
SmaI CCC'GGG
Cuts at: 0 94 213 406 612
Size: 94 119 193 206
Fragments arranged by size:
206 193 119 94
StyI C'CwwG_G
Cuts at: 0 29 612
Size: 29 583
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 530 612
Size: 530 82
XmaI C'CCGG_G
Cuts at: 0 92 211 404 612
Size: 92 119 193 208
Fragments arranged by size:
208 193 119 92
XmnI GAAnn'nnTTC
Cuts at: 0 220 612
Size: 220 392
ZraI GAC'GTC
Cuts at: 0 399 612
Size: 399 213
Enzymes that do cut:
AatII AcuI AgeI AlwNI AvaI BanI BanII BbeI
BlpI BpmI Bpu10I BsaHI BsaWI BseYI BsiEI BsiHKAI
Bsp1286I BsrBI BsrFI BssSI BstEII BtgI EaeI EagI
EciI EcoICRI Eco57MI EcoO109I FalI HaeII HincII HpaI
KasI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI PflMI PpuMI PstI PvuII SacI SacII SfcI
SfoI SmaI StyI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaXI BseRI BsgI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI
BssHII BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EarI EcoNI
EcoRI EcoRV FseI FspI FspAI Hin4I HindIII KpnI
MfeI MluI MscI NdeI NheI NruI NsiI NspI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI TstI Tth111I XbaI XhoI