(Linear) (Six-base) MAPSORT of: KIBB8882.seq  Check: 4564  from: 1  to: 546



KIBB8882.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AccI GT'mk_AC

Cuts at:      0     380     479     546 
   Size:        380      99      67

  Fragments arranged by size: 

                 380      99      67
 
AfeI AGC'GCT

Cuts at:      0      82     546 
   Size:         82     464
 
AlwNI CAG_nnn'CTG

Cuts at:      0     318     546 
   Size:        318     228
 
ApoI r'AATT_y

Cuts at:      0      49     546 
   Size:         49     497
 
AvaI C'yCGr_G

Cuts at:      0      60     152     341     440     546 
   Size:         60      92     189      99     106

  Fragments arranged by size: 

                 189     106      99      92      60
 
BanI G'GyrC_C

Cuts at:      0     305     546 
   Size:        305     241
 
BanII G_rGCy'C

Cuts at:      0     545     546 
   Size:        545       1
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     332     366     546 
   Size:        332      34     180

  Fragments arranged by size: 

                 332     180      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      31     546 
   Size:         31     515
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     112     421     546 
   Size:        112     309     125

  Fragments arranged by size: 

                 309     125     112
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     546 
   Size:        310     236
 
Bpu10I CC'TnA_GC

Cuts at:      0     199     546 
   Size:        199     347
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     535     538     546 
   Size:        535       3       8

  Fragments arranged by size: 

                 535       8       3
 
BseYI C'CCAG_C

Cuts at:      0     162     166     311     546 
   Size:        162       4     145     235

  Fragments arranged by size: 

                 235     162     145       4
 
Bsp1286I G_dGCh'C

Cuts at:      0     545     546 
   Size:        545       1
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     293     546 
   Size:        293     253
 
BsrBI CCG'CTC

Cuts at:      0      11     546 
   Size:         11     535
 
BstYI r'GATC_y

Cuts at:      0     110     546 
   Size:        110     436
 
BtgI C'CryG_G

Cuts at:      0     132     327     546 
   Size:        132     195     219

  Fragments arranged by size: 

                 219     195     132
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     466     546 
   Size:        466      80
 
EaeI y'GGCC_r

Cuts at:      0      32     447     546 
   Size:         32     415      99

  Fragments arranged by size: 

                 415      99      32
 
EarI CTCTTCn'nnn_

Cuts at:      0     101     512     546 
   Size:        101     411      34

  Fragments arranged by size: 

                 411     101      34
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     546 
   Size:        310     236
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     289     546 
   Size:        289     257
 
EcoO109I rG'GnC_Cy

Cuts at:      0     188     546 
   Size:        188     358
 
HaeII r_GCGC'y

Cuts at:      0      84     318     546 
   Size:         84     234     228

  Fragments arranged by size: 

                 234     228      84
 
HincII GTy'rAC

Cuts at:      0     381     546 
   Size:        381     165
 
MspA1I CmG'CkG

Cuts at:      0     166     546 
   Size:        166     380
 
NcoI C'CATG_G

Cuts at:      0     132     546 
   Size:        132     414
 
NspI r_CATG'y

Cuts at:      0      25     546 
   Size:         25     521
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     318     546 
   Size:        318     228
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     202     234     546 
   Size:        202      32     312

  Fragments arranged by size: 

                 312     202      32
 
PvuII CAG'CTG

Cuts at:      0     166     546 
   Size:        166     380
 
SalI G'TCGA_C

Cuts at:      0     379     546 
   Size:        379     167
 
StuI AGG'CCT

Cuts at:      0      95     546 
   Size:         95     451
 
StyI C'CwwG_G

Cuts at:      0     132     546 
   Size:        132     414
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      61     546 
   Size:         61     485
 

 Enzymes that do cut:

     AccI     AfeI    AlwNI     ApoI     AvaI     BanI    BanII     BcgI
     BglI     BplI     BpmI   Bpu10I    BseRI    BseYI Bsp1286I    BspMI
    BsrBI    BstYI     BtgI     DrdI     EaeI     EarI  Eco57MI    EcoNI
 EcoO109I    HaeII   HincII   MspA1I     NcoI     NspI    PflMI     PpiI
    PvuII     SalI     StuI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI    BciVI
     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     EagI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI