(Linear) (Six-base) MAPSORT of: KIBB8854.seq  Check: 395  from: 1  to: 246



KIBB8854.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

ApoI r'AATT_y

Cuts at:      0     213     246 
   Size:        213      33
 
Bme1580I G_kGCm'C

Cuts at:      0     185     240     246 
   Size:        185      55       6

  Fragments arranged by size: 

                 185      55       6
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     246 
   Size:         48     198
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      46      76     246 
   Size:         46      30     170

  Fragments arranged by size: 

                 170      46      30
 
BseYI C'CCAG_C

Cuts at:      0      32     169     246 
   Size:         32     137      77

  Fragments arranged by size: 

                 137      77      32
 
Bsp1286I G_dGCh'C

Cuts at:      0     185     240     246 
   Size:        185      55       6

  Fragments arranged by size: 

                 185      55       6
 
BssSI C'ACGA_G

Cuts at:      0     184     246 
   Size:        184      62
 
BstEII G'GTnAC_C

Cuts at:      0     198     246 
   Size:        198      48
 
EaeI y'GGCC_r

Cuts at:      0       2     246 
   Size:          2     244
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     246 
   Size:         48     198
 
EcoRI G'AATT_C

Cuts at:      0     213     246 
   Size:        213      33
 
HaeII r_GCGC'y

Cuts at:      0      29     246 
   Size:         29     217
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      46      78     246 
   Size:         46      32     168

  Fragments arranged by size: 

                 168      46      32
 
MscI TGG'CCA

Cuts at:      0       4     246 
   Size:          4     242
 
PstI C_TGCA'G

Cuts at:      0     212     246 
   Size:        212      34
 
SfcI C'TryA_G

Cuts at:      0      87     208     246 
   Size:         87     121      38

  Fragments arranged by size: 

                 121      87      38
 

 Enzymes that do cut:

     ApoI Bme1580I     BpmI    BsaXI    BseYI Bsp1286I    BssSI   BstEII
     EaeI  Eco57MI    EcoRI    HaeII    Hin4I     MscI     PstI     SfcI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI