Restriction map for
pF1KB8838
(Linear) (Six-base) MAPSORT of: KIBB8838.seq Check: 5145 from: 1 to: 1389
KIBB8838.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 318 1389
Size: 318 1071
AatII G_ACGT'C
Cuts at: 0 1130 1389
Size: 1130 259
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 1389
Size: 184 1205
AgeI A'CCGG_T
Cuts at: 0 1099 1389
Size: 1099 290
AhdI GACnn_n'nnGTC
Cuts at: 0 375 1389
Size: 375 1014
AleI CACnn'nnGTG
Cuts at: 0 1054 1389
Size: 1054 335
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1072 1389
Size: 1072 317
AlwNI CAG_nnn'CTG
Cuts at: 0 246 315 828 1226 1389
Size: 246 69 513 398 163
Fragments arranged by size:
513 398 246 163 69
ApaI G_GGCC'C
Cuts at: 0 193 801 1137 1389
Size: 193 608 336 252
Fragments arranged by size:
608 336 252 193
ApoI r'AATT_y
Cuts at: 0 1141 1389
Size: 1141 248
AvaI C'yCGr_G
Cuts at: 0 250 1037 1389
Size: 250 787 352
Fragments arranged by size:
787 352 250
BamHI G'GATC_C
Cuts at: 0 546 1389
Size: 546 843
BanI G'GyrC_C
Cuts at: 0 55 340 1096 1389
Size: 55 285 756 293
Fragments arranged by size:
756 293 285 55
BanII G_rGCy'C
Cuts at: 0 67 193 801 973 1111 1137 1389
Size: 67 126 608 172 138 26 252
Fragments arranged by size:
608 252 172 138 126 67 26
BbeI G_GCGC'C
Cuts at: 0 59 1389
Size: 59 1330
BglI GCCn_nnn'nGGC
Cuts at: 0 427 1389
Size: 427 962
BlpI GC'TnA_GC
Cuts at: 0 1066 1389
Size: 1066 323
Bme1580I G_kGCm'C
Cuts at: 0 193 286 801 1099 1137 1389
Size: 193 93 515 298 38 252
Fragments arranged by size:
515 298 252 193 93 38
BmgBI CAC'GTC
Cuts at: 0 372 984 1389
Size: 372 612 405
Fragments arranged by size:
612 405 372
BmrI ACTGGGnnnn_n'
Cuts at: 0 773 838 1389
Size: 773 65 551
Fragments arranged by size:
773 551 65
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1246 1389
Size: 1246 143
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 295 306 1168 1192 1389
Size: 187 108 11 862 24 197
Fragments arranged by size:
862 197 187 108 24 11
Bpu10I CC'TnA_GC
Cuts at: 0 1204 1389
Size: 1204 185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 1389
Size: 100 1289
BsaI GGTCTCn'nnnn_
Cuts at: 0 718 1389
Size: 718 671
BsaHI Gr'CG_yC
Cuts at: 0 56 1127 1389
Size: 56 1071 262
Fragments arranged by size:
1071 262 56
BsaWI w'CCGG_w
Cuts at: 0 1099 1389
Size: 1099 290
BseYI C'CCAG_C
Cuts at: 0 5 279 1336 1389
Size: 5 274 1057 53
Fragments arranged by size:
1057 274 53 5
BsiEI CG_ry'CG
Cuts at: 0 47 133 567 1063 1389
Size: 47 86 434 496 326
Fragments arranged by size:
496 434 326 86 47
BsiHKAI G_wGCw'C
Cuts at: 0 67 1389
Size: 67 1322
BsmI GAATG_Cn'
Cuts at: 0 395 1389
Size: 395 994
BsmBI CGTCTCn'nnnn_
Cuts at: 0 990 1389
Size: 990 399
Bsp1286I G_dGCh'C
Cuts at: 0 67 193 286 801 973 1099 1111 1137
Size: 67 126 93 515 172 126 12 26
Cuts at: 1137 1389
Size: 252
Fragments arranged by size:
515 252 172 126 126 93 67 26
12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 318 1197 1389
Size: 318 879 192
Fragments arranged by size:
879 318 192
BsrBI CCG'CTC
Cuts at: 0 629 835 1389
Size: 629 206 554
Fragments arranged by size:
629 554 206
BsrFI r'CCGG_y
Cuts at: 0 132 496 1099 1389
Size: 132 364 603 290
Fragments arranged by size:
603 364 290 132
BssHII G'CGCG_C
Cuts at: 0 391 1389
Size: 391 998
BstXI CCAn_nnnn'nTGG
Cuts at: 0 330 519 1389
Size: 330 189 870
Fragments arranged by size:
870 330 189
BstYI r'GATC_y
Cuts at: 0 546 587 996 1389
Size: 546 41 409 393
Fragments arranged by size:
546 409 393 41
BtgI C'CryG_G
Cuts at: 0 41 570 965 1362 1389
Size: 41 529 395 397 27
Fragments arranged by size:
529 397 395 41 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 372 1389
Size: 372 1017
BtsI GCAGTG_nn'
Cuts at: 0 1299 1389
Size: 1299 90
DraIII CAC_nnn'GTG
Cuts at: 0 347 1389
Size: 347 1042
EaeI y'GGCC_r
Cuts at: 0 130 491 521 573 1389
Size: 130 361 30 52 816
Fragments arranged by size:
816 361 130 52 30
EagI C'GGCC_G
Cuts at: 0 130 1389
Size: 130 1259
EarI CTCTTCn'nnn_
Cuts at: 0 1123 1185 1389
Size: 1123 62 204
Fragments arranged by size:
1123 204 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 675 1152 1389
Size: 675 477 237
Fragments arranged by size:
675 477 237
EcoICRI GAG'CTC
Cuts at: 0 65 1389
Size: 65 1324
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 187 295 306 1168 1192 1389
Size: 184 3 108 11 862 24 197
Fragments arranged by size:
862 197 184 108 24 11 3
EcoO109I rG'GnC_Cy
Cuts at: 0 189 190 798 1186 1342 1389
Size: 189 1 608 388 156 47
Fragments arranged by size:
608 388 189 156 47 1
HaeII r_GCGC'y
Cuts at: 0 40 59 270 655 1389
Size: 40 19 211 385 734
Fragments arranged by size:
734 385 211 40 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 361 393 937 969 1389
Size: 361 32 544 32 420
Fragments arranged by size:
544 420 361 32 32
HincII GTy'rAC
Cuts at: 0 291 1389
Size: 291 1098
KasI G'GCGC_C
Cuts at: 0 55 1389
Size: 55 1334
MscI TGG'CCA
Cuts at: 0 493 523 575 1389
Size: 493 30 52 814
Fragments arranged by size:
814 493 52 30
MslI CAynn'nnrTG
Cuts at: 0 1054 1090 1273 1389
Size: 1054 36 183 116
Fragments arranged by size:
1054 183 116 36
MspA1I CmG'CkG
Cuts at: 0 43 91 108 534 1389
Size: 43 48 17 426 855
Fragments arranged by size:
855 426 48 43 17
NaeI GCC'GGC
Cuts at: 0 134 1389
Size: 134 1255
NarI GG'CG_CC
Cuts at: 0 56 1389
Size: 56 1333
NcoI C'CATG_G
Cuts at: 0 570 1362 1389
Size: 570 792 27
Fragments arranged by size:
792 570 27
NgoMIV G'CCGG_C
Cuts at: 0 132 1389
Size: 132 1257
PasI CC'CwG_GG
Cuts at: 0 761 1389
Size: 761 628
PflMI CCAn_nnn'nTGG
Cuts at: 0 828 1389
Size: 828 561
PfoI T'CCnGG_A
Cuts at: 0 917 992 1145 1389
Size: 917 75 153 244
Fragments arranged by size:
917 244 153 75
PpuMI rG'GwC_Cy
Cuts at: 0 1186 1389
Size: 1186 203
PspOMI G'GGCC_C
Cuts at: 0 189 797 1133 1389
Size: 189 608 336 256
Fragments arranged by size:
608 336 256 189
PstI C_TGCA'G
Cuts at: 0 1356 1389
Size: 1356 33
PvuII CAG'CTG
Cuts at: 0 534 1389
Size: 534 855
SacI G_AGCT'C
Cuts at: 0 67 1389
Size: 67 1322
SacII CC_GC'GG
Cuts at: 0 44 1389
Size: 44 1345
SapI GCTCTTCn'nnn_
Cuts at: 0 1123 1389
Size: 1123 266
SbfI CC_TGCA'GG
Cuts at: 0 1356 1389
Size: 1356 33
SfcI C'TryA_G
Cuts at: 0 1352 1389
Size: 1352 37
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 427 1389
Size: 427 962
SfoI GGC'GCC
Cuts at: 0 57 1389
Size: 57 1332
SgrAI Cr'CCGG_yG
Cuts at: 0 496 1389
Size: 496 893
SmlI C'TyrA_G
Cuts at: 0 115 1037 1389
Size: 115 922 352
Fragments arranged by size:
922 352 115
StuI AGG'CCT
Cuts at: 0 1358 1379 1389
Size: 1358 21 10
Fragments arranged by size:
1358 21 10
StyI C'CwwG_G
Cuts at: 0 570 842 1362 1389
Size: 570 272 520 27
Fragments arranged by size:
570 520 272 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1239 1312 1389
Size: 1239 73 77
Fragments arranged by size:
1239 77 73
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 538 570 913 945 1389
Size: 538 32 343 32 444
Fragments arranged by size:
538 444 343 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 211 486 1389
Size: 211 275 903
Fragments arranged by size:
903 275 211
XhoI C'TCGA_G
Cuts at: 0 1037 1389
Size: 1037 352
XmnI GAAnn'nnTTC
Cuts at: 0 1277 1389
Size: 1277 112
ZraI GAC'GTC
Cuts at: 0 1128 1389
Size: 1128 261
Enzymes that do cut:
AarI AatII AcuI AgeI AhdI AleI AlfI AlwNI
ApaI ApoI AvaI BamHI BanI BanII BbeI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrBI BsrFI BssHII BstXI BstYI BtgI
BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I HaeII Hin4I HincII KasI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV PasI PflMI PfoI
PpuMI PspOMI PstI PvuII SacI SacII SapI SbfI
SfcI SfiI SfoI SgrAI SmlI StuI StyI TaqII
TstI XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AloI ApaLI
AscI AseI AsiSI AvrII BaeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BmtI BsaAI BsaBI BsaXI
BseRI BsgI BsiWI BspEI BspHI BsrDI BsrGI BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI
DrdI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI
SanDI ScaI SexAI SgfI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TatI Tth111I XbaI XmaI