Restriction map for
pF1KB8837
(Linear) (Six-base) MAPSORT of: KIBB8837.seq Check: 5818 from: 1 to: 1389
KIBB8837.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 942 958 1389
Size: 942 16 431
Fragments arranged by size:
942 431 16
AccI GT'mk_AC
Cuts at: 0 386 1142 1389
Size: 386 756 247
Fragments arranged by size:
756 386 247
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 375 537 1389
Size: 375 162 852
Fragments arranged by size:
852 375 162
AflII C'TTAA_G
Cuts at: 0 1342 1389
Size: 1342 47
AleI CACnn'nnGTG
Cuts at: 0 505 682 1389
Size: 505 177 707
Fragments arranged by size:
707 505 177
AlwNI CAG_nnn'CTG
Cuts at: 0 135 837 1389
Size: 135 702 552
Fragments arranged by size:
702 552 135
BanI G'GyrC_C
Cuts at: 0 24 719 1057 1389
Size: 24 695 338 332
Fragments arranged by size:
695 338 332 24
BanII G_rGCy'C
Cuts at: 0 1282 1389
Size: 1282 107
BbeI G_GCGC'C
Cuts at: 0 723 1061 1389
Size: 723 338 328
Fragments arranged by size:
723 338 328
BbvCI CC'TCA_GC
Cuts at: 0 1178 1389
Size: 1178 211
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1311 1345 1389
Size: 1311 34 44
Fragments arranged by size:
1311 44 34
BglI GCCn_nnn'nGGC
Cuts at: 0 619 728 943 1389
Size: 619 109 215 446
Fragments arranged by size:
619 446 215 109
BmgBI CAC'GTC
Cuts at: 0 318 1389
Size: 318 1071
BmrI ACTGGGnnnn_n'
Cuts at: 0 1038 1389
Size: 1038 351
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 112 184 250 335 778 1138 1231
Size: 91 21 72 66 85 443 360 93
Cuts at: 1231 1389
Size: 158
Fragments arranged by size:
443 360 158 93 91 85 72 66
21
Bpu10I CC'TnA_GC
Cuts at: 0 1178 1389
Size: 1178 211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 787 1389
Size: 787 602
BsaI GGTCTCn'nnnn_
Cuts at: 0 265 423 439 578 1017 1389
Size: 265 158 16 139 439 372
Fragments arranged by size:
439 372 265 158 139 16
BsaHI Gr'CG_yC
Cuts at: 0 173 263 720 824 1058 1184 1389
Size: 173 90 457 104 234 126 205
Fragments arranged by size:
457 234 205 173 126 104 90
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 694 724 1389
Size: 694 30 665
Fragments arranged by size:
694 665 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 1389
Size: 49 1340
BseYI C'CCAG_C
Cuts at: 0 536 1187 1389
Size: 536 651 202
Fragments arranged by size:
651 536 202
BsiEI CG_ry'CG
Cuts at: 0 544 860 1389
Size: 544 316 529
Fragments arranged by size:
544 529 316
BsiHKAI G_wGCw'C
Cuts at: 0 701 1389
Size: 701 688
Bsp1286I G_dGCh'C
Cuts at: 0 701 1282 1389
Size: 701 581 107
Fragments arranged by size:
701 581 107
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 341 372 713 942 958 983 1327 1389
Size: 341 31 341 229 16 25 344 62
Fragments arranged by size:
344 341 341 229 62 31 25 16
BsrBI CCG'CTC
Cuts at: 0 600 992 1389
Size: 600 392 397
Fragments arranged by size:
600 397 392
BsrFI r'CCGG_y
Cuts at: 0 343 613 657 716 840 1389
Size: 343 270 44 59 124 549
Fragments arranged by size:
549 343 270 124 59 44
BstAPI GCAn_nnn'nTGC
Cuts at: 0 705 1389
Size: 705 684
BstEII G'GTnAC_C
Cuts at: 0 100 970 1389
Size: 100 870 419
Fragments arranged by size:
870 419 100
BstXI CCAn_nnnn'nTGG
Cuts at: 0 54 1068 1389
Size: 54 1014 321
Fragments arranged by size:
1014 321 54
BstYI r'GATC_y
Cuts at: 0 464 578 1389
Size: 464 114 811
Fragments arranged by size:
811 464 114
BtgI C'CryG_G
Cuts at: 0 20 146 1389
Size: 20 126 1243
Fragments arranged by size:
1243 126 20
BtsI GCAGTG_nn'
Cuts at: 0 889 1389
Size: 889 500
DraIII CAC_nnn'GTG
Cuts at: 0 895 1389
Size: 895 494
EaeI y'GGCC_r
Cuts at: 0 56 455 524 541 857 935 944 1001
Size: 56 399 69 17 316 78 9 57
Cuts at: 1001 1250 1389
Size: 249 139
Fragments arranged by size:
399 316 249 139 78 69 57 56
17 9
EagI C'GGCC_G
Cuts at: 0 541 857 1389
Size: 541 316 532
Fragments arranged by size:
541 532 316
EarI CTCTTCn'nnn_
Cuts at: 0 1376 1389
Size: 1376 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 148 1389
Size: 148 1241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 112 184 250 335 375 537 778
Size: 91 21 72 66 85 40 162 241
Cuts at: 778 1138 1231 1389
Size: 360 93 158
Fragments arranged by size:
360 241 162 158 93 91 85 72
66 40 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 109 379 472 1083 1389
Size: 109 270 93 611 306
Fragments arranged by size:
611 306 270 109 93
EcoO109I rG'GnC_Cy
Cuts at: 0 75 240 330 516 834 1206 1389
Size: 75 165 90 186 318 372 183
Fragments arranged by size:
372 318 186 183 165 90 75
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 507 1389
Size: 507 882
FspI TGC'GCA
Cuts at: 0 82 499 1389
Size: 82 417 890
Fragments arranged by size:
890 417 82
FspAI rTGC'GCAy
Cuts at: 0 499 1389
Size: 499 890
HaeII r_GCGC'y
Cuts at: 0 723 1061 1139 1389
Size: 723 338 78 250
Fragments arranged by size:
723 338 250 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 694 726 913 945 964 996 1389
Size: 694 32 187 32 19 32 393
Fragments arranged by size:
694 393 187 32 32 32 19
KasI G'GCGC_C
Cuts at: 0 719 1057 1389
Size: 719 338 332
Fragments arranged by size:
719 338 332
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 671 1076 1389
Size: 671 405 313
Fragments arranged by size:
671 405 313
MscI TGG'CCA
Cuts at: 0 58 937 1003 1252 1389
Size: 58 879 66 249 137
Fragments arranged by size:
879 249 137 66 58
MslI CAynn'nnrTG
Cuts at: 0 52 505 682 1066 1096 1389
Size: 52 453 177 384 30 293
Fragments arranged by size:
453 384 293 177 52 30
MspA1I CmG'CkG
Cuts at: 0 431 460 540 565 1361 1389
Size: 431 29 80 25 796 28
Fragments arranged by size:
796 431 80 29 28 25
NaeI GCC'GGC
Cuts at: 0 345 615 659 1389
Size: 345 270 44 730
Fragments arranged by size:
730 345 270 44
NarI GG'CG_CC
Cuts at: 0 720 1058 1389
Size: 720 338 331
Fragments arranged by size:
720 338 331
NgoMIV G'CCGG_C
Cuts at: 0 343 613 657 1389
Size: 343 270 44 732
Fragments arranged by size:
732 343 270 44
NotI GC'GGCC_GC
Cuts at: 0 857 1389
Size: 857 532
PasI CC'CwG_GG
Cuts at: 0 847 1389
Size: 847 542
PfoI T'CCnGG_A
Cuts at: 0 128 1389
Size: 128 1261
PpuMI rG'GwC_Cy
Cuts at: 0 240 330 516 1389
Size: 240 90 186 873
Fragments arranged by size:
873 240 186 90
PstI C_TGCA'G
Cuts at: 0 575 1324 1389
Size: 575 749 65
Fragments arranged by size:
749 575 65
PvuII CAG'CTG
Cuts at: 0 1361 1389
Size: 1361 28
SbfI CC_TGCA'GG
Cuts at: 0 575 1324 1389
Size: 575 749 65
Fragments arranged by size:
749 575 65
SfcI C'TryA_G
Cuts at: 0 571 1320 1389
Size: 571 749 69
Fragments arranged by size:
749 571 69
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 943 1389
Size: 943 446
SfoI GGC'GCC
Cuts at: 0 721 1059 1389
Size: 721 338 330
Fragments arranged by size:
721 338 330
SgrAI Cr'CCGG_yG
Cuts at: 0 716 1389
Size: 716 673
SmlI C'TyrA_G
Cuts at: 0 802 1342 1389
Size: 802 540 47
Fragments arranged by size:
802 540 47
StuI AGG'CCT
Cuts at: 0 763 1389
Size: 763 626
StyI C'CwwG_G
Cuts at: 0 59 503 1389
Size: 59 444 886
Fragments arranged by size:
886 444 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 317 550 972 1389
Size: 317 233 422 417
Fragments arranged by size:
422 417 317 233
TatI w'GTAC_w
Cuts at: 0 1346 1389
Size: 1346 43
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 66 892 910 1389
Size: 66 826 18 479
Fragments arranged by size:
826 479 66 18
Enzymes that do cut:
AarI AccI AcuI AflII AleI AlwNI BanI BanII
BbeI BbvCI BcgI BglI BmgBI BmrI BpmI Bpu10I
BpuEI BsaI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrFI BstAPI BstEII BstXI BstYI
BtgI BtsI DraIII EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I KasI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NotI
PasI PfoI PpuMI PstI PvuII SbfI SfcI SfiI
SfoI SgrAI SmlI StuI StyI TaqII TatI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AhdI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmtI BplI BsaAI BsaBI BsaWI BsgI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII
BssSI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EcoICRI EcoRI EcoRV FseI HincII HindIII HpaI
KpnI MfeI MluI NcoI NdeI NheI NruI NsiI
NspI PacI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI ScaI SexAI SgfI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI