(Linear) (Six-base) MAPSORT of: KIBB8816.seq  Check: 8672  from: 1  to: 1137



KIBB8816.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AccI GT'mk_AC

Cuts at:      0     605    1137 
   Size:        605     532
 
Acc65I G'GTAC_C

Cuts at:      0     224    1137 
   Size:        224     913
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     272     766    1137 
   Size:        272     494     371

  Fragments arranged by size: 

                 494     371     272
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     737    1137 
   Size:        737     400
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1103    1135    1137 
   Size:       1103      32       2

  Fragments arranged by size: 

                1103      32       2
 
AlwNI CAG_nnn'CTG

Cuts at:      0     789    1137 
   Size:        789     348
 
ApaI G_GGCC'C

Cuts at:      0     849    1137 
   Size:        849     288
 
ApoI r'AATT_y

Cuts at:      0     804    1015    1090    1137 
   Size:        804     211      75      47

  Fragments arranged by size: 

                 804     211      75      47
 
AvaI C'yCGr_G

Cuts at:      0     296     521    1137 
   Size:        296     225     616

  Fragments arranged by size: 

                 616     296     225
 
BamHI G'GATC_C

Cuts at:      0     525    1137 
   Size:        525     612
 
BanI G'GyrC_C

Cuts at:      0      55     224    1137 
   Size:         55     169     913

  Fragments arranged by size: 

                 913     169      55
 
BanII G_rGCy'C

Cuts at:      0     240     849     871    1137 
   Size:        240     609      22     266

  Fragments arranged by size: 

                 609     266     240      22
 
BbeI G_GCGC'C

Cuts at:      0      59    1137 
   Size:         59    1078
 
BbvCI CC'TCA_GC

Cuts at:      0     116    1137 
   Size:        116    1021
 
BfrBI ATG'CAT

Cuts at:      0     457    1137 
   Size:        457     680
 
Bme1580I G_kGCm'C

Cuts at:      0     303     849    1137 
   Size:        303     546     288

  Fragments arranged by size: 

                 546     303     288
 
BmgBI CAC'GTC

Cuts at:      0     304    1137 
   Size:        304     833
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     874    1137 
   Size:        874     263
 
Bpu10I CC'TnA_GC

Cuts at:      0     116     841    1137 
   Size:        116     725     296

  Fragments arranged by size: 

                 725     296     116
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     344     588    1137 
   Size:        344     244     549

  Fragments arranged by size: 

                 549     344     244
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     496    1137 
   Size:        496     641
 
BsaHI Gr'CG_yC

Cuts at:      0      56    1137 
   Size:         56    1081
 
BseYI C'CCAG_C

Cuts at:      0      23     145    1137 
   Size:         23     122     992

  Fragments arranged by size: 

                 992     122      23
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     679    1137 
   Size:        679     458
 
BsmI GAATG_Cn'

Cuts at:      0     582    1137 
   Size:        582     555
 
Bsp1286I G_dGCh'C

Cuts at:      0     240     303     849     871    1137 
   Size:        240      63     546      22     266

  Fragments arranged by size: 

                 546     266     240      63      22
 
BsrDI GCAATG_nn'

Cuts at:      0     207    1137 
   Size:        207     930
 
BstEII G'GTnAC_C

Cuts at:      0      27    1137 
   Size:         27    1110
 
BstYI r'GATC_y

Cuts at:      0     124     525    1137 
   Size:        124     401     612

  Fragments arranged by size: 

                 612     401     124
 
BstZ17I GTA'TAC

Cuts at:      0     606    1137 
   Size:        606     531
 
DraI TTT'AAA

Cuts at:      0    1010    1137 
   Size:       1010     127
 
EaeI y'GGCC_r

Cuts at:      0    1097    1137 
   Size:       1097      40
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     272     766    1137 
   Size:        272     494     371

  Fragments arranged by size: 

                 494     371     272
 
EcoO109I rG'GnC_Cy

Cuts at:      0     845    1137 
   Size:        845     292
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     922    1012    1137 
   Size:        922      90     125

  Fragments arranged by size: 

                 922     125      90
 
HaeII r_GCGC'y

Cuts at:      0      59    1137 
   Size:         59    1078
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     109     141    1137 
   Size:        109      32     996

  Fragments arranged by size: 

                 996     109      32
 
KasI G'GCGC_C

Cuts at:      0      55    1137 
   Size:         55    1082
 
KpnI G_GTAC'C

Cuts at:      0     228    1137 
   Size:        228     909
 
MfeI C'AATT_G

Cuts at:      0     497    1137 
   Size:        497     640
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1065    1137 
   Size:       1065      72
 
MscI TGG'CCA

Cuts at:      0    1099    1137 
   Size:       1099      38
 
MspA1I CmG'CkG

Cuts at:      0     760    1137 
   Size:        760     377
 
NarI GG'CG_CC

Cuts at:      0      56    1137 
   Size:         56    1081
 
NdeI CA'TA_TG

Cuts at:      0    1115    1137 
   Size:       1115      22
 
NsiI A_TGCA'T

Cuts at:      0     459    1137 
   Size:        459     678
 
NspI r_CATG'y

Cuts at:      0     720    1137 
   Size:        720     417
 
PspOMI G'GGCC_C

Cuts at:      0     845    1137 
   Size:        845     292
 
SfoI GGC'GCC

Cuts at:      0      57    1137 
   Size:         57    1080
 
SmaI CCC'GGG

Cuts at:      0     298    1137 
   Size:        298     839
 
SmlI C'TyrA_G

Cuts at:      0     359     567    1137 
   Size:        359     208     570

  Fragments arranged by size: 

                 570     359     208
 
StuI AGG'CCT

Cuts at:      0      38    1137 
   Size:         38    1099
 
StyI C'CwwG_G

Cuts at:      0     232    1137 
   Size:        232     905
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      15     475    1137 
   Size:         15     460     662

  Fragments arranged by size: 

                 662     460      15
 
XmaI C'CCGG_G

Cuts at:      0     296    1137 
   Size:        296     841
 
XmnI GAAnn'nnTTC

Cuts at:      0     902     979    1137 
   Size:        902      77     158

  Fragments arranged by size: 

                 902     158      77
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AlfI     AloI    AlwNI     ApaI     ApoI
     AvaI    BamHI     BanI    BanII     BbeI    BbvCI    BfrBI Bme1580I
    BmgBI     BmrI   Bpu10I    BpuEI     BsaI    BsaHI    BseYI     BsgI
     BsmI Bsp1286I    BsrDI   BstEII    BstYI  BstZ17I     DraI     EaeI
  Eco57MI EcoO109I     FalI    HaeII    Hin4I     KasI     KpnI     MfeI
     MmeI     MscI   MspA1I     NarI     NdeI     NsiI     NspI   PspOMI
     SfoI     SmaI     SmlI     StuI     StyI    TaqII     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbsI
     BcgI    BciVI     BclI     BglI    BglII     BlpI     BmtI     BplI
     BpmI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BsiEI  BsiHKAI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI    BstXI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII
  HindIII     HpaI     MluI     MslI     NaeI     NcoI   NgoMIV     NheI
     NotI     NruI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     ZraI