(Linear) (Six-base) MAPSORT of: KIBB8671.seq  Check: 1638  from: 1  to: 1188



KIBB8671.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

AccI GT'mk_AC

Cuts at:      0     677    1188 
   Size:        677     511
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     125     439    1055    1188 
   Size:        125     314     616     133

  Fragments arranged by size: 

                 616     314     133     125
 
AflIII A'CryG_T

Cuts at:      0     370    1188 
   Size:        370     818
 
AlwNI CAG_nnn'CTG

Cuts at:      0     315    1188 
   Size:        315     873
 
ApaI G_GGCC'C

Cuts at:      0     485    1188 
   Size:        485     703
 
ApoI r'AATT_y

Cuts at:      0     248    1156    1188 
   Size:        248     908      32

  Fragments arranged by size: 

                 908     248      32
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     826     859    1188 
   Size:        826      33     329

  Fragments arranged by size: 

                 826     329      33
 
BanII G_rGCy'C

Cuts at:      0     300     485    1188 
   Size:        300     185     703

  Fragments arranged by size: 

                 703     300     185
 
BbvCI CC'TCA_GC

Cuts at:      0     827    1188 
   Size:        827     361
 
Bme1580I G_kGCm'C

Cuts at:      0     485    1188 
   Size:        485     703
 
Bpu10I CC'TnA_GC

Cuts at:      0     827    1188 
   Size:        827     361
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     790    1188 
   Size:        790     398
 
BsaAI yAC'GTr

Cuts at:      0     373    1188 
   Size:        373     815
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     963     993    1188 
   Size:        963      30     195

  Fragments arranged by size: 

                 963     195      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     533     536     844     971    1188 
   Size:        533       3     308     127     217

  Fragments arranged by size: 

                 533     308     217     127       3
 
BseYI C'CCAG_C

Cuts at:      0     892    1188 
   Size:        892     296
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     147    1188 
   Size:        147    1041
 
BsiHKAI G_wGCw'C

Cuts at:      0     300    1188 
   Size:        300     888
 
BsmI GAATG_Cn'

Cuts at:      0     327    1188 
   Size:        327     861
 
Bsp1286I G_dGCh'C

Cuts at:      0     300     485    1188 
   Size:        300     185     703

  Fragments arranged by size: 

                 703     300     185
 
BspHI T'CATG_A

Cuts at:      0     609    1188 
   Size:        609     579
 
BstYI r'GATC_y

Cuts at:      0     417    1188 
   Size:        417     771
 
Bsu36I CC'TnA_GG

Cuts at:      0     983    1188 
   Size:        983     205
 
BtsI GCAGTG_nn'

Cuts at:      0     926    1188 
   Size:        926     262
 
DraI TTT'AAA

Cuts at:      0    1059    1188 
   Size:       1059     129
 
DraIII CAC_nnn'GTG

Cuts at:      0     868    1188 
   Size:        868     320
 
EarI CTCTTCn'nnn_

Cuts at:      0     443    1188 
   Size:        443     745
 
EcoICRI GAG'CTC

Cuts at:      0     298    1188 
   Size:        298     890
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     125     439    1055    1188 
   Size:        125     314     616     133

  Fragments arranged by size: 

                 616     314     133     125
 
EcoO109I rG'GnC_Cy

Cuts at:      0     312     794    1188 
   Size:        312     482     394

  Fragments arranged by size: 

                 482     394     312
 
EcoRV GAT'ATC

Cuts at:      0     393    1188 
   Size:        393     795
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     963     995    1130    1162    1188 
   Size:        963      32     135      32      26

  Fragments arranged by size: 

                 963     135      32      32      26
 
HincII GTy'rAC

Cuts at:      0     125    1188 
   Size:        125    1063
 
HpaI GTT'AAC

Cuts at:      0     125    1188 
   Size:        125    1063
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      63     933    1188 
   Size:         63     870     255

  Fragments arranged by size: 

                 870     255      63
 
MslI CAynn'nnrTG

Cuts at:      0     341    1188 
   Size:        341     847
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      46      97    1188 
   Size:         46      51    1091

  Fragments arranged by size: 

                1091      51      46
 
PmlI CAC'GTG

Cuts at:      0     373    1188 
   Size:        373     815
 
PspOMI G'GGCC_C

Cuts at:      0     481    1188 
   Size:        481     707
 
PstI C_TGCA'G

Cuts at:      0     933     993    1188 
   Size:        933      60     195

  Fragments arranged by size: 

                 933     195      60
 
SacI G_AGCT'C

Cuts at:      0     300    1188 
   Size:        300     888
 
SapI GCTCTTCn'nnn_

Cuts at:      0     443    1188 
   Size:        443     745
 
SfcI C'TryA_G

Cuts at:      0     929     989    1188 
   Size:        929      60     199

  Fragments arranged by size: 

                 929     199      60
 
SmlI C'TyrA_G

Cuts at:      0     805    1188 
   Size:        805     383
 
StyI C'CwwG_G

Cuts at:      0     797    1188 
   Size:        797     391
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     738    1188 
   Size:        738     450
 
TatI w'GTAC_w

Cuts at:      0    1100    1188 
   Size:       1100      88
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     156     188    1188 
   Size:        156      32    1000

  Fragments arranged by size: 

                1000     156      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     452    1188 
   Size:        452     736
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     ApaI     ApoI     BaeI    BanII
    BbvCI Bme1580I   Bpu10I    BpuEI    BsaAI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BstYI   Bsu36I     BtsI
     DraI   DraIII     EarI  EcoICRI  Eco57MI EcoO109I    EcoRV    Hin4I
   HincII     HpaI     MmeI     MslI    PflMI     PmlI   PspOMI     PstI
     SacI     SapI     SfcI     SmlI     StyI    TaqII     TatI     TstI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII    BamHI     BanI     BbeI     BbsI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
     BpmI     BsaI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI    BsmBI
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     ClaI
    CspCI     DrdI     EaeI     EagI     EciI    EcoNI    EcoRI     FalI
     FseI     FspI    FspAI    HaeII  HindIII     KasI     KpnI     MfeI
     MluI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI