Restriction map for
pF1KB8669
(Linear) (Six-base) MAPSORT of: KIBB8669.seq Check: 8782 from: 1 to: 1770
KIBB8669.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 759 1770
Size: 759 1011
Acc65I G'GTAC_C
Cuts at: 0 674 923 1770
Size: 674 249 847
Fragments arranged by size:
847 674 249
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1134 1465 1770
Size: 1134 331 305
Fragments arranged by size:
1134 331 305
AlwNI CAG_nnn'CTG
Cuts at: 0 508 586 711 871 969 1770
Size: 508 78 125 160 98 801
Fragments arranged by size:
801 508 160 125 98 78
ApaI G_GGCC'C
Cuts at: 0 865 1615 1770
Size: 865 750 155
Fragments arranged by size:
865 750 155
ApaLI G'TGCA_C
Cuts at: 0 1063 1770
Size: 1063 707
AvaI C'yCGr_G
Cuts at: 0 131 1522 1770
Size: 131 1391 248
Fragments arranged by size:
1391 248 131
AvrII C'CTAG_G
Cuts at: 0 297 1770
Size: 297 1473
BamHI G'GATC_C
Cuts at: 0 956 1770
Size: 956 814
BanI G'GyrC_C
Cuts at: 0 95 240 341 660 674 891 923 1113
Size: 95 145 101 319 14 217 32 190
Cuts at: 1113 1337 1770
Size: 224 433
Fragments arranged by size:
433 319 224 217 190 145 101 95
32 14
BanII G_rGCy'C
Cuts at: 0 476 865 1304 1547 1615 1770
Size: 476 389 439 243 68 155
Fragments arranged by size:
476 439 389 243 155 68
BbeI G_GCGC'C
Cuts at: 0 1341 1770
Size: 1341 429
BbsI GAAGACnn'nnnn_
Cuts at: 0 1078 1080 1770
Size: 1078 2 690
Fragments arranged by size:
1078 690 2
BciVI GTATCCnnnnn_n'
Cuts at: 0 509 1770
Size: 509 1261
BclI T'GATC_A
Cuts at: 0 1424 1770
Size: 1424 346
BglI GCCn_nnn'nGGC
Cuts at: 0 329 365 842 1129 1610 1770
Size: 329 36 477 287 481 160
Fragments arranged by size:
481 477 329 287 160 36
BglII A'GATC_T
Cuts at: 0 1019 1290 1770
Size: 1019 271 480
Fragments arranged by size:
1019 480 271
BlpI GC'TnA_GC
Cuts at: 0 428 1546 1770
Size: 428 1118 224
Fragments arranged by size:
1118 428 224
Bme1580I G_kGCm'C
Cuts at: 0 39 245 344 633 865 894 1067 1118
Size: 39 206 99 289 232 29 173 51
Cuts at: 1118 1583 1615 1770
Size: 465 32 155
Fragments arranged by size:
465 289 232 206 173 155 99 51
39 32 29
BmgBI CAC'GTC
Cuts at: 0 1515 1770
Size: 1515 255
BmrI ACTGGGnnnn_n'
Cuts at: 0 602 620 627 1656 1770
Size: 602 18 7 1029 114
Fragments arranged by size:
1029 602 114 18 7
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 582 1293 1770
Size: 582 711 477
Fragments arranged by size:
711 582 477
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 794 893 1240 1275 1770
Size: 457 337 99 347 35 495
Fragments arranged by size:
495 457 347 337 99 35
Bpu10I CC'TnA_GC
Cuts at: 0 470 557 1770
Size: 470 87 1213
Fragments arranged by size:
1213 470 87
BsaI GGTCTCn'nnnn_
Cuts at: 0 54 1770
Size: 54 1716
BsaAI yAC'GTr
Cuts at: 0 1410 1770
Size: 1410 360
BsaHI Gr'CG_yC
Cuts at: 0 1338 1440 1770
Size: 1338 102 330
Fragments arranged by size:
1338 330 102
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 754 1770
Size: 754 1016
BseYI C'CCAG_C
Cuts at: 0 236 378 529 678 701 719 730 1770
Size: 236 142 151 149 23 18 11 1040
Fragments arranged by size:
1040 236 151 149 142 23 18 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1129 1770
Size: 1129 641
BsiEI CG_ry'CG
Cuts at: 0 1334 1638 1770
Size: 1334 304 132
Fragments arranged by size:
1334 304 132
BsiHKAI G_wGCw'C
Cuts at: 0 1067 1304 1646 1688 1770
Size: 1067 237 342 42 82
Fragments arranged by size:
1067 342 237 82 42
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1090 1770
Size: 1090 680
Bsp1286I G_dGCh'C
Cuts at: 0 39 245 344 476 633 865 894 1067
Size: 39 206 99 132 157 232 29 173
Cuts at: 1067 1118 1304 1547 1583 1615 1646 1688 1770
Size: 51 186 243 36 32 31 42 82
Fragments arranged by size:
243 232 206 186 173 157 132 99
82 51 42 39 36 32 31 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 636 759 1034 1770
Size: 636 123 275 736
Fragments arranged by size:
736 636 275 123
BsrBI CCG'CTC
Cuts at: 0 854 1251 1770
Size: 854 397 519
Fragments arranged by size:
854 519 397
BsrFI r'CCGG_y
Cuts at: 0 29 845 1770
Size: 29 816 925
Fragments arranged by size:
925 816 29
BstXI CCAn_nnnn'nTGG
Cuts at: 0 832 1770
Size: 832 938
BstYI r'GATC_y
Cuts at: 0 956 1019 1290 1770
Size: 956 63 271 480
Fragments arranged by size:
956 480 271 63
BtgI C'CryG_G
Cuts at: 0 1328 1556 1679 1770
Size: 1328 228 123 91
Fragments arranged by size:
1328 228 123 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1393 1536 1770
Size: 1393 143 234
Fragments arranged by size:
1393 234 143
BtsI GCAGTG_nn'
Cuts at: 0 275 527 741 1770
Size: 275 252 214 1029
Fragments arranged by size:
1029 275 252 214
DraIII CAC_nnn'GTG
Cuts at: 0 898 1770
Size: 898 872
DrdI GACnn_nn'nnGTC
Cuts at: 0 1012 1770
Size: 1012 758
EaeI y'GGCC_r
Cuts at: 0 366 524 843 900 1121 1194 1226 1331
Size: 366 158 319 57 221 73 32 105
Cuts at: 1331 1635 1661 1770
Size: 304 26 109
Fragments arranged by size:
366 319 304 221 158 109 105 73
57 32 26
EagI C'GGCC_G
Cuts at: 0 1331 1635 1770
Size: 1331 304 135
Fragments arranged by size:
1331 304 135
EarI CTCTTCn'nnn_
Cuts at: 0 220 1770
Size: 220 1550
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 347 864 1770
Size: 347 517 906
Fragments arranged by size:
906 517 347
EcoICRI GAG'CTC
Cuts at: 0 1302 1770
Size: 1302 468
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 794 893 1134 1240 1275 1465 1770
Size: 457 337 99 241 106 35 190 305
Fragments arranged by size:
457 337 305 241 190 106 99 35
EcoO109I rG'GnC_Cy
Cuts at: 0 301 861 966 1612 1770
Size: 301 560 105 646 158
Fragments arranged by size:
646 560 301 158 105
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1309 1770
Size: 1309 461
FspI TGC'GCA
Cuts at: 0 289 1770
Size: 289 1481
HaeII r_GCGC'y
Cuts at: 0 747 1341 1504 1770
Size: 747 594 163 266
Fragments arranged by size:
747 594 266 163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 478 510 1159 1191 1770
Size: 478 32 649 32 579
Fragments arranged by size:
649 579 478 32 32
KasI G'GCGC_C
Cuts at: 0 1337 1770
Size: 1337 433
KpnI G_GTAC'C
Cuts at: 0 678 927 1770
Size: 678 249 843
Fragments arranged by size:
843 678 249
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1016 1770
Size: 1016 754
MscI TGG'CCA
Cuts at: 0 368 1663 1770
Size: 368 1295 107
Fragments arranged by size:
1295 368 107
MslI CAynn'nnrTG
Cuts at: 0 154 395 401 1555 1770
Size: 154 241 6 1154 215
Fragments arranged by size:
1154 241 215 154 6
MspA1I CmG'CkG
Cuts at: 0 529 945 1770
Size: 529 416 825
Fragments arranged by size:
825 529 416
NaeI GCC'GGC
Cuts at: 0 31 847 1770
Size: 31 816 923
Fragments arranged by size:
923 816 31
NarI GG'CG_CC
Cuts at: 0 1338 1770
Size: 1338 432
NgoMIV G'CCGG_C
Cuts at: 0 29 845 1770
Size: 29 816 925
Fragments arranged by size:
925 816 29
PflMI CCAn_nnn'nTGG
Cuts at: 0 871 948 1770
Size: 871 77 822
Fragments arranged by size:
871 822 77
PfoI T'CCnGG_A
Cuts at: 0 223 1217 1770
Size: 223 994 553
Fragments arranged by size:
994 553 223
PspOMI G'GGCC_C
Cuts at: 0 861 1611 1770
Size: 861 750 159
Fragments arranged by size:
861 750 159
PstI C_TGCA'G
Cuts at: 0 513 965 1770
Size: 513 452 805
Fragments arranged by size:
805 513 452
PvuII CAG'CTG
Cuts at: 0 945 1770
Size: 945 825
SacI G_AGCT'C
Cuts at: 0 1304 1770
Size: 1304 466
SbfI CC_TGCA'GG
Cuts at: 0 965 1770
Size: 965 805
SexAI A'CCwGG_T
Cuts at: 0 670 1770
Size: 670 1100
SfcI C'TryA_G
Cuts at: 0 509 961 1770
Size: 509 452 809
Fragments arranged by size:
809 509 452
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 365 842 1129 1770
Size: 365 477 287 641
Fragments arranged by size:
641 477 365 287
SfoI GGC'GCC
Cuts at: 0 1339 1770
Size: 1339 431
SmaI CCC'GGG
Cuts at: 0 133 1770
Size: 133 1637
StuI AGG'CCT
Cuts at: 0 323 1417 1770
Size: 323 1094 353
Fragments arranged by size:
1094 353 323
StyI C'CwwG_G
Cuts at: 0 297 983 1058 1770
Size: 297 686 75 712
Fragments arranged by size:
712 686 297 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 128 159 193 228 352 678 715 1770
Size: 128 31 34 35 124 326 37 1055
Fragments arranged by size:
1055 326 128 124 37 35 34 31
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 352 1770
Size: 352 1418
XmaI C'CCGG_G
Cuts at: 0 131 1770
Size: 131 1639
Enzymes that do cut:
AarI Acc65I AcuI AlwNI ApaI ApaLI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BsaI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrFI BstXI BstYI BtgI
BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I FalI FspI HaeII Hin4I KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
PflMI PfoI PspOMI PstI PvuII SacI SbfI SexAI
SfcI SfiI SfoI SmaI StuI StyI TaqII XcmI
XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApoI AscI AseI AsiSI BaeI
BbvCI BcgI BfrBI BmtI BpuEI BsaBI BsaWI BsaXI
BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI
EcoNI EcoRI EcoRV FseI FspAI HincII HindIII HpaI
MfeI MluI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI ScaI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TatI TstI Tth111I XbaI
XhoI XmnI ZraI