Restriction map for
pF1KB8653
(Linear) (Six-base) MAPSORT of: KIBB8653.seq Check: 5045 from: 1 to: 702
KIBB8653.orf
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 250 702
Size: 250 452
Acc65I G'GTAC_C
Cuts at: 0 512 702
Size: 512 190
AflIII A'CryG_T
Cuts at: 0 266 702
Size: 266 436
AvaI C'yCGr_G
Cuts at: 0 440 702
Size: 440 262
BamHI G'GATC_C
Cuts at: 0 618 702
Size: 618 84
BanI G'GyrC_C
Cuts at: 0 37 512 523 637 702
Size: 37 475 11 114 65
Fragments arranged by size:
475 114 65 37 11
BanII G_rGCy'C
Cuts at: 0 605 677 702
Size: 605 72 25
Fragments arranged by size:
605 72 25
BbeI G_GCGC'C
Cuts at: 0 41 527 702
Size: 41 486 175
Fragments arranged by size:
486 175 41
BglI GCCn_nnn'nGGC
Cuts at: 0 343 532 702
Size: 343 189 170
Fragments arranged by size:
343 189 170
BmgBI CAC'GTC
Cuts at: 0 267 702
Size: 267 435
BmrI ACTGGGnnnn_n'
Cuts at: 0 620 702
Size: 620 82
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 558 702
Size: 558 144
BsaI GGTCTCn'nnnn_
Cuts at: 0 399 702
Size: 399 303
BsaAI yAC'GTr
Cuts at: 0 597 702
Size: 597 105
BsaHI Gr'CG_yC
Cuts at: 0 38 524 702
Size: 38 486 178
Fragments arranged by size:
486 178 38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 139 169 702
Size: 139 30 533
Fragments arranged by size:
533 139 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 181 702
Size: 181 521
BseYI C'CCAG_C
Cuts at: 0 562 702
Size: 562 140
BsiHKAI G_wGCw'C
Cuts at: 0 230 702
Size: 230 472
BsiWI C'GTAC_G
Cuts at: 0 14 702
Size: 14 688
Bsp1286I G_dGCh'C
Cuts at: 0 230 605 677 702
Size: 230 375 72 25
Fragments arranged by size:
375 230 72 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 250 381 702
Size: 250 131 321
Fragments arranged by size:
321 250 131
BsrBI CCG'CTC
Cuts at: 0 22 702
Size: 22 680
BsrFI r'CCGG_y
Cuts at: 0 625 651 702
Size: 625 26 51
Fragments arranged by size:
625 51 26
BstYI r'GATC_y
Cuts at: 0 618 702
Size: 618 84
BtgI C'CryG_G
Cuts at: 0 218 702
Size: 218 484
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 435 702
Size: 435 267
DrdI GACnn_nn'nnGTC
Cuts at: 0 328 702
Size: 328 374
EaeI y'GGCC_r
Cuts at: 0 215 377 653 702
Size: 215 162 276 49
Fragments arranged by size:
276 215 162 49
EarI CTCTTCn'nnn_
Cuts at: 0 544 702
Size: 544 158
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 558 702
Size: 558 144
HaeII r_GCGC'y
Cuts at: 0 41 155 527 702
Size: 41 114 372 175
Fragments arranged by size:
372 175 114 41
KasI G'GCGC_C
Cuts at: 0 37 523 702
Size: 37 486 179
Fragments arranged by size:
486 179 37
KpnI G_GTAC'C
Cuts at: 0 516 702
Size: 516 186
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 702
Size: 344 358
MscI TGG'CCA
Cuts at: 0 379 702
Size: 379 323
MslI CAynn'nnrTG
Cuts at: 0 112 702
Size: 112 590
MspA1I CmG'CkG
Cuts at: 0 510 702
Size: 510 192
NaeI GCC'GGC
Cuts at: 0 653 702
Size: 653 49
NarI GG'CG_CC
Cuts at: 0 38 524 702
Size: 38 486 178
Fragments arranged by size:
486 178 38
NgoMIV G'CCGG_C
Cuts at: 0 651 702
Size: 651 51
NspI r_CATG'y
Cuts at: 0 582 702
Size: 582 120
PasI CC'CwG_GG
Cuts at: 0 678 702
Size: 678 24
PflMI CCAn_nnn'nTGG
Cuts at: 0 386 702
Size: 386 316
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 124 156 702
Size: 124 32 546
Fragments arranged by size:
546 124 32
PstI C_TGCA'G
Cuts at: 0 205 702
Size: 205 497
SfcI C'TryA_G
Cuts at: 0 201 702
Size: 201 501
SfoI GGC'GCC
Cuts at: 0 39 525 702
Size: 39 486 177
Fragments arranged by size:
486 177 39
SmaI CCC'GGG
Cuts at: 0 442 702
Size: 442 260
SphI G_CATG'C
Cuts at: 0 582 702
Size: 582 120
SrfI GCCC'GGGC
Cuts at: 0 442 702
Size: 442 260
StyI C'CwwG_G
Cuts at: 0 404 702
Size: 404 298
TatI w'GTAC_w
Cuts at: 0 269 702
Size: 269 433
XmaI C'CCGG_G
Cuts at: 0 440 702
Size: 440 262
Enzymes that do cut:
AarI Acc65I AflIII AvaI BamHI BanI BanII BbeI
BglI BmgBI BmrI BpmI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsiHKAI BsiWI Bsp1286I BspMI BsrBI BsrFI
BstYI BtgI BtgZI DrdI EaeI EarI Eco57MI HaeII
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PflMI PsrI PstI SfcI SfoI
SmaI SphI SrfI StyI TatI XmaI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmtI BplI Bpu10I
BpuEI BsaBI BsaWI BsgI BsiEI BsmI BsmBI BspEI
BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII
EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI MfeI
MluI NcoI NdeI NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmlI SnaBI SpeI SspI StuI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmnI ZraI