Restriction map for
pF1KB8646
(Linear) (Six-base) MAPSORT of: KIBB8646.seq Check: 5110 from: 1 to: 717
KIBB8646.orf
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AatII G_ACGT'C
Cuts at: 0 19 717
Size: 19 698
AscI GG'CGCG_CC
Cuts at: 0 555 717
Size: 555 162
AvaI C'yCGr_G
Cuts at: 0 219 304 390 701 717
Size: 219 85 86 311 16
Fragments arranged by size:
311 219 86 85 16
BamHI G'GATC_C
Cuts at: 0 215 717
Size: 215 502
BanII G_rGCy'C
Cuts at: 0 228 311 717
Size: 228 83 406
Fragments arranged by size:
406 228 83
BbsI GAAGACnn'nnnn_
Cuts at: 0 577 717
Size: 577 140
BbvCI CC'TCA_GC
Cuts at: 0 115 717
Size: 115 602
Bme1580I G_kGCm'C
Cuts at: 0 640 717
Size: 640 77
BmrI ACTGGGnnnn_n'
Cuts at: 0 443 646 717
Size: 443 203 71
Fragments arranged by size:
443 203 71
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 717
Size: 190 527
Bpu10I CC'TnA_GC
Cuts at: 0 115 717
Size: 115 602
BsaHI Gr'CG_yC
Cuts at: 0 16 717
Size: 16 701
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 273 303 717
Size: 273 30 414
Fragments arranged by size:
414 273 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 240 717
Size: 240 477
BseYI C'CCAG_C
Cuts at: 0 33 717
Size: 33 684
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 717
Size: 25 692
BsiHKAI G_wGCw'C
Cuts at: 0 708 717
Size: 708 9
BsmI GAATG_Cn'
Cuts at: 0 60 717
Size: 60 657
BsmBI CGTCTCn'nnnn_
Cuts at: 0 53 717
Size: 53 664
Bsp1286I G_dGCh'C
Cuts at: 0 228 311 640 708 717
Size: 228 83 329 68 9
Fragments arranged by size:
329 228 83 68 9
BspHI T'CATG_A
Cuts at: 0 365 717
Size: 365 352
BsrBI CCG'CTC
Cuts at: 0 540 717
Size: 540 177
BsrFI r'CCGG_y
Cuts at: 0 329 717
Size: 329 388
BssHII G'CGCG_C
Cuts at: 0 555 717
Size: 555 162
BstXI CCAn_nnnn'nTGG
Cuts at: 0 168 717
Size: 168 549
BstYI r'GATC_y
Cuts at: 0 215 717
Size: 215 502
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 157 457 717
Size: 157 300 260
Fragments arranged by size:
300 260 157
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 717
Size: 190 527
EcoNI CCTnn'n_nnAGG
Cuts at: 0 585 717
Size: 585 132
EcoO109I rG'GnC_Cy
Cuts at: 0 321 643 717
Size: 321 322 74
Fragments arranged by size:
322 321 74
FspI TGC'GCA
Cuts at: 0 487 717
Size: 487 230
HaeII r_GCGC'y
Cuts at: 0 115 688 717
Size: 115 573 29
Fragments arranged by size:
573 115 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 273 305 353 385 717
Size: 273 32 48 32 332
Fragments arranged by size:
332 273 48 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 692 717
Size: 316 376 25
Fragments arranged by size:
376 316 25
MslI CAynn'nnrTG
Cuts at: 0 163 717
Size: 163 554
MspA1I CmG'CkG
Cuts at: 0 624 717
Size: 624 93
NspI r_CATG'y
Cuts at: 0 168 468 550 717
Size: 168 300 82 167
Fragments arranged by size:
300 168 167 82
PpuMI rG'GwC_Cy
Cuts at: 0 321 717
Size: 321 396
PstI C_TGCA'G
Cuts at: 0 233 717
Size: 233 484
PvuII CAG'CTG
Cuts at: 0 624 717
Size: 624 93
SanDI GG'GwC_CC
Cuts at: 0 321 717
Size: 321 396
SfcI C'TryA_G
Cuts at: 0 229 717
Size: 229 488
SmaI CCC'GGG
Cuts at: 0 221 392 717
Size: 221 171 325
Fragments arranged by size:
325 221 171
SpeI A'CTAG_T
Cuts at: 0 676 717
Size: 676 41
SphI G_CATG'C
Cuts at: 0 550 717
Size: 550 167
StuI AGG'CCT
Cuts at: 0 379 717
Size: 379 338
StyI C'CwwG_G
Cuts at: 0 38 717
Size: 38 679
XmaI C'CCGG_G
Cuts at: 0 219 390 717
Size: 219 171 327
Fragments arranged by size:
327 219 171
ZraI GAC'GTC
Cuts at: 0 17 717
Size: 17 700
Enzymes that do cut:
AatII AscI AvaI BamHI BanII BbsI BbvCI Bme1580I
BmrI BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrBI BsrFI BssHII
BstXI BstYI EciI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I MmeI MslI MspA1I NspI PpuMI PstI PvuII
SanDI SfcI SmaI SpeI SphI StuI StyI XmaI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvrII BaeI BanI BbeI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI
BspEI BspMI BsrDI BsrGI BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EcoICRI EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmnI