Restriction map for
pF1KB8580
(Linear) (Six-base) MAPSORT of: KIBB8580.seq Check: 9312 from: 1 to: 1446
KIBB8580.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1353 1446
Size: 1353 93
AccI GT'mk_AC
Cuts at: 0 714 1446
Size: 714 732
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 1446
Size: 184 1262
AfeI AGC'GCT
Cuts at: 0 172 1446
Size: 172 1274
AlwNI CAG_nnn'CTG
Cuts at: 0 865 1446
Size: 865 581
ApaI G_GGCC'C
Cuts at: 0 64 107 259 1446
Size: 64 43 152 1187
Fragments arranged by size:
1187 152 64 43
ApoI r'AATT_y
Cuts at: 0 505 850 878 1421 1446
Size: 505 345 28 543 25
Fragments arranged by size:
543 505 345 28 25
AvaI C'yCGr_G
Cuts at: 0 10 938 1446
Size: 10 928 508
Fragments arranged by size:
928 508 10
BanI G'GyrC_C
Cuts at: 0 238 1446
Size: 238 1208
BanII G_rGCy'C
Cuts at: 0 64 107 259 377 1446
Size: 64 43 152 118 1069
Fragments arranged by size:
1069 152 118 64 43
BbeI G_GCGC'C
Cuts at: 0 242 1446
Size: 242 1204
BbvCI CC'TCA_GC
Cuts at: 0 47 578 1446
Size: 47 531 868
Fragments arranged by size:
868 531 47
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 947 981 1446
Size: 947 34 465
Fragments arranged by size:
947 465 34
BglI GCCn_nnn'nGGC
Cuts at: 0 388 1446
Size: 388 1058
BlpI GC'TnA_GC
Cuts at: 0 202 1446
Size: 202 1244
Bme1580I G_kGCm'C
Cuts at: 0 64 107 259 1446
Size: 64 43 152 1187
Fragments arranged by size:
1187 152 64 43
BmgBI CAC'GTC
Cuts at: 0 344 369 1446
Size: 344 25 1077
Fragments arranged by size:
1077 344 25
BmrI ACTGGGnnnn_n'
Cuts at: 0 232 1446
Size: 232 1214
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 712 1446
Size: 712 734
Bpu10I CC'TnA_GC
Cuts at: 0 47 578 1446
Size: 47 531 868
Fragments arranged by size:
868 531 47
BsaHI Gr'CG_yC
Cuts at: 0 37 157 239 314 1446
Size: 37 120 82 75 1132
Fragments arranged by size:
1132 120 82 75 37
BsaWI w'CCGG_w
Cuts at: 0 824 1446
Size: 824 622
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 22 52 716 746 1446
Size: 22 30 664 30 700
Fragments arranged by size:
700 664 30 30 22
BseYI C'CCAG_C
Cuts at: 0 25 250 480 737 1022 1446
Size: 25 225 230 257 285 424
Fragments arranged by size:
424 285 257 230 225 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 695 1446
Size: 656 39 751
Fragments arranged by size:
751 656 39
BsiEI CG_ry'CG
Cuts at: 0 296 350 1446
Size: 296 54 1096
Fragments arranged by size:
1096 296 54
BsmI GAATG_Cn'
Cuts at: 0 991 1446
Size: 991 455
Bsp1286I G_dGCh'C
Cuts at: 0 64 107 259 377 1446
Size: 64 43 152 118 1069
Fragments arranged by size:
1069 152 118 64 43
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 850 1353 1446
Size: 850 503 93
Fragments arranged by size:
850 503 93
BsrFI r'CCGG_y
Cuts at: 0 295 1186 1446
Size: 295 891 260
Fragments arranged by size:
891 295 260
BstAPI GCAn_nnn'nTGC
Cuts at: 0 535 634 865 1446
Size: 535 99 231 581
Fragments arranged by size:
581 535 231 99
BstYI r'GATC_y
Cuts at: 0 1073 1102 1247 1446
Size: 1073 29 145 199
Fragments arranged by size:
1073 199 145 29
BtgI C'CryG_G
Cuts at: 0 65 1446
Size: 65 1381
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 221 1446
Size: 221 1225
DraIII CAC_nnn'GTG
Cuts at: 0 499 673 1446
Size: 499 174 773
Fragments arranged by size:
773 499 174
DrdI GACnn_nn'nnGTC
Cuts at: 0 156 1446
Size: 156 1290
EaeI y'GGCC_r
Cuts at: 0 293 335 347 1446
Size: 293 42 12 1099
Fragments arranged by size:
1099 293 42 12
EagI C'GGCC_G
Cuts at: 0 293 347 1446
Size: 293 54 1099
Fragments arranged by size:
1099 293 54
EarI CTCTTCn'nnn_
Cuts at: 0 190 1446
Size: 190 1256
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 67 1446
Size: 67 1379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 1446
Size: 184 1262
EcoO109I rG'GnC_Cy
Cuts at: 0 61 103 104 255 862 1446
Size: 61 42 1 151 607 584
Fragments arranged by size:
607 584 151 61 42 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 189 1446
Size: 189 1257
HaeII r_GCGC'y
Cuts at: 0 174 242 305 394 1446
Size: 174 68 63 89 1052
Fragments arranged by size:
1052 174 89 68 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 22 54 956 988 1029 1061 1446
Size: 22 32 902 32 41 32 385
Fragments arranged by size:
902 385 41 32 32 32 22
HincII GTy'rAC
Cuts at: 0 715 927 1446
Size: 715 212 519
Fragments arranged by size:
715 519 212
KasI G'GCGC_C
Cuts at: 0 238 1446
Size: 238 1208
MfeI C'AATT_G
Cuts at: 0 842 1446
Size: 842 604
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1065 1446
Size: 1065 381
MscI TGG'CCA
Cuts at: 0 337 1446
Size: 337 1109
MspA1I CmG'CkG
Cuts at: 0 25 67 123 250 1022 1446
Size: 25 42 56 127 772 424
Fragments arranged by size:
772 424 127 56 42 25
NaeI GCC'GGC
Cuts at: 0 297 1446
Size: 297 1149
NarI GG'CG_CC
Cuts at: 0 239 1446
Size: 239 1207
NgoMIV G'CCGG_C
Cuts at: 0 295 1446
Size: 295 1151
NspI r_CATG'y
Cuts at: 0 1277 1446
Size: 1277 169
PasI CC'CwG_GG
Cuts at: 0 1170 1446
Size: 1170 276
PpuMI rG'GwC_Cy
Cuts at: 0 862 1446
Size: 862 584
PspOMI G'GGCC_C
Cuts at: 0 60 103 255 1446
Size: 60 43 152 1191
Fragments arranged by size:
1191 152 60 43
PstI C_TGCA'G
Cuts at: 0 122 1446
Size: 122 1324
PvuII CAG'CTG
Cuts at: 0 25 1446
Size: 25 1421
SacII CC_GC'GG
Cuts at: 0 68 1446
Size: 68 1378
SalI G'TCGA_C
Cuts at: 0 713 1446
Size: 713 733
SfcI C'TryA_G
Cuts at: 0 118 1079 1118 1446
Size: 118 961 39 328
Fragments arranged by size:
961 328 118 39
SfoI GGC'GCC
Cuts at: 0 240 1446
Size: 240 1206
StyI C'CwwG_G
Cuts at: 0 152 485 1446
Size: 152 333 961
Fragments arranged by size:
961 333 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 971 1446
Size: 971 475
XbaI T'CTAG_A
Cuts at: 0 597 1446
Size: 597 849
Enzymes that do cut:
AarI AccI AcuI AfeI AlwNI ApaI ApoI AvaI
BanI BanII BbeI BbvCI BcgI BglI BlpI Bme1580I
BmgBI BmrI BplI Bpu10I BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsmI Bsp1286I BspMI BsrFI BstAPI BstYI
BtgI BtgZI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I FalI HaeII Hin4I HincII KasI MfeI
MmeI MscI MspA1I NaeI NarI NgoMIV NspI PasI
PpuMI PspOMI PstI PvuII SacII SalI SfcI SfoI
StyI TaqII XbaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BciVI BclI BfrBI BglII BmtI BpmI
BpuEI BsaI BsaAI BsaBI BseRI BsiHKAI BsiWI BsmBI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI KpnI MluI MslI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI Tth111I XcmI XhoI XmaI XmnI
ZraI