(Linear) (Six-base) MAPSORT of: KIBB8566.seq  Check: 4158  from: 1  to: 762



KIBB8566.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

AclI AA'CG_TT

Cuts at:      0     724     762 
   Size:        724      38
 
AflIII A'CryG_T

Cuts at:      0     504     762 
   Size:        504     258
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      86     762 
   Size:         86     676
 
ApoI r'AATT_y

Cuts at:      0      37     762 
   Size:         37     725
 
AseI AT'TA_AT

Cuts at:      0     718     762 
   Size:        718      44
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     234     267     762 
   Size:        234      33     495

  Fragments arranged by size: 

                 495     234      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     563     762 
   Size:        563     199
 
BbvCI CC'TCA_GC

Cuts at:      0      31     762 
   Size:         31     731
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     481     515     762 
   Size:        481      34     247

  Fragments arranged by size: 

                 481     247      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     195     472     762 
   Size:        195     277     290

  Fragments arranged by size: 

                 290     277     195
 
BclI T'GATC_A

Cuts at:      0      86     762 
   Size:         86     676
 
BglII A'GATC_T

Cuts at:      0     384     762 
   Size:        384     378
 
BmgBI CAC'GTC

Cuts at:      0     505     762 
   Size:        505     257
 
Bpu10I CC'TnA_GC

Cuts at:      0      31     762 
   Size:         31     731
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     430     762 
   Size:        430     332
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     226     762 
   Size:        226     536
 
BsrDI GCAATG_nn'

Cuts at:      0     739     762 
   Size:        739      23
 
BssSI C'ACGA_G

Cuts at:      0     753     762 
   Size:        753       9
 
BstYI r'GATC_y

Cuts at:      0     384     762 
   Size:        384     378
 
Bsu36I CC'TnA_GG

Cuts at:      0     587     762 
   Size:        587     175
 
BtgI C'CryG_G

Cuts at:      0       5     762 
   Size:          5     757
 
BtsI GCAGTG_nn'

Cuts at:      0     690     762 
   Size:        690      72
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      26     762 
   Size:         26     736
 
EcoRV GAT'ATC

Cuts at:      0     282     762 
   Size:        282     480
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     513     762 
   Size:        513     249
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     136     168     762 
   Size:        136      32     594

  Fragments arranged by size: 

                 594     136      32
 
HincII GTy'rAC

Cuts at:      0     294     762 
   Size:        294     468
 
HpaI GTT'AAC

Cuts at:      0     294     762 
   Size:        294     468
 
MspA1I CmG'CkG

Cuts at:      0       7      30     265     762 
   Size:          7      23     235     497

  Fragments arranged by size: 

                 497     235      23       7
 
PstI C_TGCA'G

Cuts at:      0     264     685     762 
   Size:        264     421      77

  Fragments arranged by size: 

                 421     264      77
 
PvuII CAG'CTG

Cuts at:      0     265     762 
   Size:        265     497
 
SacII CC_GC'GG

Cuts at:      0       8     762 
   Size:          8     754
 
SfcI C'TryA_G

Cuts at:      0     260     266     681     762 
   Size:        260       6     415      81

  Fragments arranged by size: 

                 415     260      81       6
 
SspI AAT'ATT

Cuts at:      0     693     762 
   Size:        693      69
 
TatI w'GTAC_w

Cuts at:      0     164     762 
   Size:        164     598
 
XmnI GAAnn'nnTTC

Cuts at:      0     706     762 
   Size:        706      56
 

 Enzymes that do cut:

     AclI   AflIII     AlfI     ApoI     AseI     BaeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BglII    BmgBI   Bpu10I    BseRI    BsmBI
    BsrDI    BssSI    BstYI   Bsu36I     BtgI     BtsI     EciI    EcoRV
     FalI    Hin4I   HincII     HpaI   MspA1I     PstI    PvuII    SacII
     SfcI     SspI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII    BamHI     BanI    BanII     BbeI    BfrBI     BglI
     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FseI     FspI
    FspAI    HaeII  HindIII     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI