Restriction map for
pF1KB8556
(Linear) (Six-base) MAPSORT of: KIBB8556.seq Check: 4982 from: 1 to: 2730
KIBB8556.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AatII G_ACGT'C
Cuts at: 0 1397 2730
Size: 1397 1333
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 871 1618 2214 2234 2730
Size: 871 747 596 20 496
Fragments arranged by size:
871 747 596 496 20
AhdI GACnn_n'nnGTC
Cuts at: 0 1473 2730
Size: 1473 1257
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2166 2198 2730
Size: 2166 32 532
Fragments arranged by size:
2166 532 32
AlwNI CAG_nnn'CTG
Cuts at: 0 500 2607 2730
Size: 500 2107 123
Fragments arranged by size:
2107 500 123
ApaI G_GGCC'C
Cuts at: 0 954 2730
Size: 954 1776
ApaLI G'TGCA_C
Cuts at: 0 245 2730
Size: 245 2485
ApoI r'AATT_y
Cuts at: 0 45 282 304 388 2730
Size: 45 237 22 84 2342
Fragments arranged by size:
2342 237 84 45 22
AvaI C'yCGr_G
Cuts at: 0 915 1312 2422 2449 2562 2730
Size: 915 397 1110 27 113 168
Fragments arranged by size:
1110 915 397 168 113 27
BamHI G'GATC_C
Cuts at: 0 1851 2067 2730
Size: 1851 216 663
Fragments arranged by size:
1851 663 216
BanI G'GyrC_C
Cuts at: 0 6 661 810 1717 2730
Size: 6 655 149 907 1013
Fragments arranged by size:
1013 907 655 149 6
BanII G_rGCy'C
Cuts at: 0 582 596 954 1313 1713 2730
Size: 582 14 358 359 400 1017
Fragments arranged by size:
1017 582 400 359 358 14
BbeI G_GCGC'C
Cuts at: 0 10 665 2730
Size: 10 655 2065
Fragments arranged by size:
2065 655 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 652 859 1490 1874 2730
Size: 652 207 631 384 856
Fragments arranged by size:
856 652 631 384 207
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 520 554 2730
Size: 520 34 2176
Fragments arranged by size:
2176 520 34
BglI GCCn_nnn'nGGC
Cuts at: 0 361 2730
Size: 361 2369
BglII A'GATC_T
Cuts at: 0 902 2730
Size: 902 1828
BlpI GC'TnA_GC
Cuts at: 0 1387 2704 2730
Size: 1387 1317 26
Fragments arranged by size:
1387 1317 26
Bme1580I G_kGCm'C
Cuts at: 0 249 533 602 815 954 2730
Size: 249 284 69 213 139 1776
Fragments arranged by size:
1776 284 249 213 139 69
BmrI ACTGGGnnnn_n'
Cuts at: 0 369 813 1176 2730
Size: 369 444 363 1554
Fragments arranged by size:
1554 444 369 363
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 604 1564 1702 2124 2730
Size: 458 146 960 138 422 606
Fragments arranged by size:
960 606 458 422 146 138
Bpu10I CC'TnA_GC
Cuts at: 0 1744 2730
Size: 1744 986
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 763 1037 1319 2730
Size: 291 472 274 282 1411
Fragments arranged by size:
1411 472 291 282 274
BsaAI yAC'GTr
Cuts at: 0 879 2730
Size: 879 1851
BsaHI Gr'CG_yC
Cuts at: 0 7 662 1394 1754 2730
Size: 7 655 732 360 976
Fragments arranged by size:
976 732 655 360 7
BsaWI w'CCGG_w
Cuts at: 0 1848 2730
Size: 1848 882
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 431 461 2730
Size: 431 30 2269
Fragments arranged by size:
2269 431 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1040 2139 2262 2345 2520 2611 2730
Size: 1040 1099 123 83 175 91 119
Fragments arranged by size:
1099 1040 175 123 119 91 83
BseYI C'CCAG_C
Cuts at: 0 26 1538 1561 1643 2374 2730
Size: 26 1512 23 82 731 356
Fragments arranged by size:
1512 731 356 82 26 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1282 2730
Size: 1282 1448
BsiHKAI G_wGCw'C
Cuts at: 0 249 1313 1703 2730
Size: 249 1064 390 1027
Fragments arranged by size:
1064 1027 390 249
BsmI GAATG_Cn'
Cuts at: 0 1553 2730
Size: 1553 1177
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1761 2730
Size: 1761 969
Bsp1286I G_dGCh'C
Cuts at: 0 249 533 582 596 602 815 954 1313
Size: 249 284 49 14 6 213 139 359
Cuts at: 1313 1703 1713 2730
Size: 390 10 1017
Fragments arranged by size:
1017 390 359 284 249 213 139 49
14 10 6
BspEI T'CCGG_A
Cuts at: 0 1848 2730
Size: 1848 882
BspHI T'CATG_A
Cuts at: 0 461 2730
Size: 461 2269
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 63 2358 2730
Size: 63 2295 372
Fragments arranged by size:
2295 372 63
BsrBI CCG'CTC
Cuts at: 0 12 710 2730
Size: 12 698 2020
Fragments arranged by size:
2020 698 12
BsrDI GCAATG_nn'
Cuts at: 0 2598 2730
Size: 2598 132
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1842 2730
Size: 1842 888
BstBI TT'CG_AA
Cuts at: 0 2098 2730
Size: 2098 632
BstXI CCAn_nnnn'nTGG
Cuts at: 0 567 2730
Size: 567 2163
BstYI r'GATC_y
Cuts at: 0 902 1851 1965 2067 2163 2181 2730
Size: 902 949 114 102 96 18 549
Fragments arranged by size:
949 902 549 114 102 96 18
BtgI C'CryG_G
Cuts at: 0 723 815 1713 1787 2007 2359 2730
Size: 723 92 898 74 220 352 371
Fragments arranged by size:
898 723 371 352 220 92 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2682 2730
Size: 2682 48
DraI TTT'AAA
Cuts at: 0 1893 2310 2730
Size: 1893 417 420
Fragments arranged by size:
1893 420 417
DraIII CAC_nnn'GTG
Cuts at: 0 253 570 2730
Size: 253 317 2160
Fragments arranged by size:
2160 317 253
DrdI GACnn_nn'nnGTC
Cuts at: 0 2521 2730
Size: 2521 209
EaeI y'GGCC_r
Cuts at: 0 176 255 557 885 956 1736 2693 2730
Size: 176 79 302 328 71 780 957 37
Fragments arranged by size:
957 780 328 302 176 79 71 37
EarI CTCTTCn'nnn_
Cuts at: 0 1226 2730
Size: 1226 1504
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 594 2653 2730
Size: 594 2059 77
Fragments arranged by size:
2059 594 77
EcoICRI GAG'CTC
Cuts at: 0 1311 2730
Size: 1311 1419
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 604 871 1564 1618 1702 2124 2214
Size: 458 146 267 693 54 84 422 90
Cuts at: 2214 2234 2730
Size: 20 496
Fragments arranged by size:
693 496 458 422 267 146 90 84
54 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 586 2730
Size: 586 2144
EcoO109I rG'GnC_Cy
Cuts at: 0 504 951 1980 2198 2427 2496 2682 2730
Size: 504 447 1029 218 229 69 186 48
Fragments arranged by size:
1029 504 447 229 218 186 69 48
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 136 921 2730
Size: 136 785 1809
Fragments arranged by size:
1809 785 136
HaeII r_GCGC'y
Cuts at: 0 10 665 2730
Size: 10 655 2065
Fragments arranged by size:
2065 655 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 431 463 1459 1491 2566 2598 2730
Size: 431 32 996 32 1075 32 132
Fragments arranged by size:
1075 996 431 132 32 32 32
HincII GTy'rAC
Cuts at: 0 1604 2730
Size: 1604 1126
KasI G'GCGC_C
Cuts at: 0 6 661 2730
Size: 6 655 2069
Fragments arranged by size:
2069 655 6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1487 2730
Size: 1487 1243
MscI TGG'CCA
Cuts at: 0 257 559 887 1738 2695 2730
Size: 257 302 328 851 957 35
Fragments arranged by size:
957 851 328 302 257 35
MslI CAynn'nnrTG
Cuts at: 0 945 2730
Size: 945 1785
MspA1I CmG'CkG
Cuts at: 0 1542 1575 1647 2378 2462 2607 2730
Size: 1542 33 72 731 84 145 123
Fragments arranged by size:
1542 731 145 123 84 72 33
NarI GG'CG_CC
Cuts at: 0 7 662 2730
Size: 7 655 2068
Fragments arranged by size:
2068 655 7
NcoI C'CATG_G
Cuts at: 0 1713 1787 2730
Size: 1713 74 943
Fragments arranged by size:
1713 943 74
NdeI CA'TA_TG
Cuts at: 0 946 2730
Size: 946 1784
NspI r_CATG'y
Cuts at: 0 294 1386 2721 2730
Size: 294 1092 1335 9
Fragments arranged by size:
1335 1092 294 9
PflMI CCAn_nnn'nTGG
Cuts at: 0 2008 2257 2730
Size: 2008 249 473
Fragments arranged by size:
2008 473 249
PpuMI rG'GwC_Cy
Cuts at: 0 504 1980 2198 2496 2682 2730
Size: 504 1476 218 298 186 48
Fragments arranged by size:
1476 504 298 218 186 48
PshAI GACnn'nnGTC
Cuts at: 0 1166 2730
Size: 1166 1564
PspOMI G'GGCC_C
Cuts at: 0 950 2730
Size: 950 1780
PspXI vC'TCGA_Gb
Cuts at: 0 1312 2730
Size: 1312 1418
PstI C_TGCA'G
Cuts at: 0 351 2538 2730
Size: 351 2187 192
Fragments arranged by size:
2187 351 192
PvuII CAG'CTG
Cuts at: 0 1542 1575 1647 2607 2730
Size: 1542 33 72 960 123
Fragments arranged by size:
1542 960 123 72 33
SacI G_AGCT'C
Cuts at: 0 1313 2730
Size: 1313 1417
SanDI GG'GwC_CC
Cuts at: 0 1980 2496 2730
Size: 1980 516 234
Fragments arranged by size:
1980 516 234
SexAI A'CCwGG_T
Cuts at: 0 1694 2730
Size: 1694 1036
SfcI C'TryA_G
Cuts at: 0 347 2534 2730
Size: 347 2187 196
Fragments arranged by size:
2187 347 196
SfoI GGC'GCC
Cuts at: 0 8 663 2730
Size: 8 655 2067
Fragments arranged by size:
2067 655 8
SmlI C'TyrA_G
Cuts at: 0 270 778 1052 1312 1334 2730
Size: 270 508 274 260 22 1396
Fragments arranged by size:
1396 508 274 270 260 22
SphI G_CATG'C
Cuts at: 0 294 2730
Size: 294 2436
SspI AAT'ATT
Cuts at: 0 1201 2730
Size: 1201 1529
StuI AGG'CCT
Cuts at: 0 2554 2730
Size: 2554 176
StyI C'CwwG_G
Cuts at: 0 156 1713 1787 2276 2730
Size: 156 1557 74 489 454
Fragments arranged by size:
1557 489 454 156 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 795 871 940 1744 2730
Size: 795 76 69 804 986
Fragments arranged by size:
986 804 795 76 69
TatI w'GTAC_w
Cuts at: 0 2386 2730
Size: 2386 344
Tth111I GACn'n_nGTC
Cuts at: 0 445 1072 2730
Size: 445 627 1658
Fragments arranged by size:
1658 627 445
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 800 1702 2730
Size: 800 902 1028
Fragments arranged by size:
1028 902 800
XhoI C'TCGA_G
Cuts at: 0 1312 2730
Size: 1312 1418
ZraI GAC'GTC
Cuts at: 0 1395 2730
Size: 1395 1335
Enzymes that do cut:
AatII AcuI AhdI AloI AlwNI ApaI ApaLI ApoI
AvaI BamHI BanI BanII BbeI BbsI BcgI BglI
BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BstAPI
BstBI BstXI BstYI BtgI BtgZI DraI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I
NarI NcoI NdeI NspI PflMI PpuMI PshAI PspOMI
PspXI PstI PvuII SacI SanDI SexAI SfcI SfoI
SmlI SphI SspI StuI StyI TaqII TatI Tth111I
XcmI XhoI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AscI AseI AsiSI AvrII BaeI BbvCI
BciVI BclI BfrBI BmgBI BmtI BplI BsaI BsaBI
BsiEI BsiWI BsrFI BsrGI BssHII BssSI BstEII BstZ17I
Bsu36I BtsI ClaI CspCI EagI EcoRI EcoRV FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI NaeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PpiI PsiI PsrI PvuI RsrII
SacII SalI SapI SbfI ScaI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SwaI TstI XbaI XmaI
XmnI