Restriction map for
pF1KB8550
(Linear) (Six-base) MAPSORT of: KIBB8550.seq Check: 9565 from: 1 to: 2718
KIBB8550.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 501 1017 2718
Size: 501 516 1701
Fragments arranged by size:
1701 516 501
AccI GT'mk_AC
Cuts at: 0 437 2718
Size: 437 2281
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 1662 2001 2718
Size: 352 1310 339 717
Fragments arranged by size:
1310 717 352 339
AfeI AGC'GCT
Cuts at: 0 2397 2718
Size: 2397 321
AgeI A'CCGG_T
Cuts at: 0 28 2718
Size: 28 2690
AleI CACnn'nnGTG
Cuts at: 0 838 2718
Size: 838 1880
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 29 2718
Size: 29 2689
AlwNI CAG_nnn'CTG
Cuts at: 0 496 573 918 2718
Size: 496 77 345 1800
Fragments arranged by size:
1800 496 345 77
ApaI G_GGCC'C
Cuts at: 0 309 2718
Size: 309 2409
ApoI r'AATT_y
Cuts at: 0 430 724 2718
Size: 430 294 1994
Fragments arranged by size:
1994 430 294
AvaI C'yCGr_G
Cuts at: 0 824 1397 2718
Size: 824 573 1321
Fragments arranged by size:
1321 824 573
AvrII C'CTAG_G
Cuts at: 0 36 2718
Size: 36 2682
BanI G'GyrC_C
Cuts at: 0 52 739 2718
Size: 52 687 1979
Fragments arranged by size:
1979 687 52
BanII G_rGCy'C
Cuts at: 0 155 309 1020 1398 2718
Size: 155 154 711 378 1320
Fragments arranged by size:
1320 711 378 155 154
BbeI G_GCGC'C
Cuts at: 0 743 2718
Size: 743 1975
BbsI GAAGACnn'nnnn_
Cuts at: 0 1575 2718
Size: 1575 1143
BbvCI CC'TCA_GC
Cuts at: 0 902 2407 2718
Size: 902 1505 311
Fragments arranged by size:
1505 902 311
BciVI GTATCCnnnnn_n'
Cuts at: 0 480 544 2718
Size: 480 64 2174
Fragments arranged by size:
2174 480 64
BglI GCCn_nnn'nGGC
Cuts at: 0 1900 2718
Size: 1900 818
Bme1580I G_kGCm'C
Cuts at: 0 309 2718
Size: 309 2409
BmgBI CAC'GTC
Cuts at: 0 315 2718
Size: 315 2403
BmrI ACTGGGnnnn_n'
Cuts at: 0 222 2718
Size: 222 2496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 1387 2173 2718
Size: 595 792 786 545
Fragments arranged by size:
792 786 595 545
Bpu10I CC'TnA_GC
Cuts at: 0 902 2407 2718
Size: 902 1505 311
Fragments arranged by size:
1505 902 311
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1249 2718
Size: 1249 1469
BsaI GGTCTCn'nnnn_
Cuts at: 0 821 846 1394 2718
Size: 821 25 548 1324
Fragments arranged by size:
1324 821 548 25
BsaAI yAC'GTr
Cuts at: 0 1101 1347 2718
Size: 1101 246 1371
Fragments arranged by size:
1371 1101 246
BsaBI GATnn'nnATC
Cuts at: 0 1006 1543 2718
Size: 1006 537 1175
Fragments arranged by size:
1175 1006 537
BsaHI Gr'CG_yC
Cuts at: 0 740 1077 1850 2718
Size: 740 337 773 868
Fragments arranged by size:
868 773 740 337
BsaWI w'CCGG_w
Cuts at: 0 28 554 2718
Size: 28 526 2164
Fragments arranged by size:
2164 526 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1427 1457 1637 1667 2718
Size: 1427 30 180 30 1051
Fragments arranged by size:
1427 1051 180 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 559 1872 2116 2718
Size: 559 1313 244 602
Fragments arranged by size:
1313 602 559 244
BseYI C'CCAG_C
Cuts at: 0 958 978 1189 2718
Size: 958 20 211 1529
Fragments arranged by size:
1529 958 211 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 9 1078 1967 2718
Size: 9 1069 889 751
Fragments arranged by size:
1069 889 751 9
BsiEI CG_ry'CG
Cuts at: 0 456 2718
Size: 456 2262
BsiHKAI G_wGCw'C
Cuts at: 0 1020 2718
Size: 1020 1698
BsmI GAATG_Cn'
Cuts at: 0 960 2718
Size: 960 1758
Bsp1286I G_dGCh'C
Cuts at: 0 155 309 1020 1398 2718
Size: 155 154 711 378 1320
Fragments arranged by size:
1320 711 378 155 154
BspHI T'CATG_A
Cuts at: 0 1547 2718
Size: 1547 1171
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 501 1017 2718
Size: 501 516 1701
Fragments arranged by size:
1701 516 501
BsrBI CCG'CTC
Cuts at: 0 591 2718
Size: 591 2127
BsrDI GCAATG_nn'
Cuts at: 0 1299 2240 2418 2718
Size: 1299 941 178 300
Fragments arranged by size:
1299 941 300 178
BsrFI r'CCGG_y
Cuts at: 0 28 2718
Size: 28 2690
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1029 1336 2718
Size: 1029 307 1382
Fragments arranged by size:
1382 1029 307
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1347 2208 2718
Size: 1347 861 510
Fragments arranged by size:
1347 861 510
BstYI r'GATC_y
Cuts at: 0 1248 1538 1999 2718
Size: 1248 290 461 719
Fragments arranged by size:
1248 719 461 290
BtgI C'CryG_G
Cuts at: 0 1916 2708 2718
Size: 1916 792 10
Fragments arranged by size:
1916 792 10
BtsI GCAGTG_nn'
Cuts at: 0 811 2294 2619 2718
Size: 811 1483 325 99
Fragments arranged by size:
1483 811 325 99
ClaI AT'CG_AT
Cuts at: 0 2665 2718
Size: 2665 53
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 717 752 2718
Size: 717 35 1966
Fragments arranged by size:
1966 717 35
EaeI y'GGCC_r
Cuts at: 0 788 1901 2718
Size: 788 1113 817
Fragments arranged by size:
1113 817 788
EarI CTCTTCn'nnn_
Cuts at: 0 844 1446 1666 1697 2718
Size: 844 602 220 31 1021
Fragments arranged by size:
1021 844 602 220 31
EcoICRI GAG'CTC
Cuts at: 0 1018 2718
Size: 1018 1700
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 595 1387 1662 2001 2173 2718
Size: 352 243 792 275 339 172 545
Fragments arranged by size:
792 545 352 339 275 243 172
EcoO109I rG'GnC_Cy
Cuts at: 0 305 306 570 1823 1953 2718
Size: 305 1 264 1253 130 765
Fragments arranged by size:
1253 765 305 264 130 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2031 2718
Size: 2031 687
HaeII r_GCGC'y
Cuts at: 0 743 2399 2718
Size: 743 1656 319
Fragments arranged by size:
1656 743 319
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 598 630 1427 1459 1917 1949 2382 2414
Size: 598 32 797 32 458 32 433 32
Cuts at: 2414 2718
Size: 304
Fragments arranged by size:
797 598 458 433 304 32 32 32
32
HincII GTy'rAC
Cuts at: 0 282 525 2718
Size: 282 243 2193
Fragments arranged by size:
2193 282 243
KasI G'GCGC_C
Cuts at: 0 739 2718
Size: 739 1979
MslI CAynn'nnrTG
Cuts at: 0 838 1645 2206 2718
Size: 838 807 561 512
Fragments arranged by size:
838 807 561 512
MspA1I CmG'CkG
Cuts at: 0 372 493 762 982 2184 2366 2588 2606
Size: 372 121 269 220 1202 182 222 18
Cuts at: 2606 2718
Size: 112
Fragments arranged by size:
1202 372 269 222 220 182 121 112
18
NarI GG'CG_CC
Cuts at: 0 740 2718
Size: 740 1978
NspI r_CATG'y
Cuts at: 0 118 288 2718
Size: 118 170 2430
Fragments arranged by size:
2430 170 118
PasI CC'CwG_GG
Cuts at: 0 989 1782 2718
Size: 989 793 936
Fragments arranged by size:
989 936 793
PfoI T'CCnGG_A
Cuts at: 0 482 1493 2150 2718
Size: 482 1011 657 568
Fragments arranged by size:
1011 657 568 482
PmlI CAC'GTG
Cuts at: 0 1101 1347 2718
Size: 1101 246 1371
Fragments arranged by size:
1371 1101 246
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2670 2702 2718
Size: 2670 32 16
Fragments arranged by size:
2670 32 16
PpuMI rG'GwC_Cy
Cuts at: 0 570 1823 1953 2718
Size: 570 1253 130 765
Fragments arranged by size:
1253 765 570 130
PshAI GACnn'nnGTC
Cuts at: 0 520 2718
Size: 520 2198
PsiI TTA'TAA
Cuts at: 0 649 2718
Size: 649 2069
PspOMI G'GGCC_C
Cuts at: 0 305 2718
Size: 305 2413
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1207 1239 2718
Size: 1207 32 1479
Fragments arranged by size:
1479 1207 32
PstI C_TGCA'G
Cuts at: 0 389 476 1025 1173 2718
Size: 389 87 549 148 1545
Fragments arranged by size:
1545 549 389 148 87
PvuII CAG'CTG
Cuts at: 0 372 493 762 982 2718
Size: 372 121 269 220 1736
Fragments arranged by size:
1736 372 269 220 121
SacI G_AGCT'C
Cuts at: 0 1020 2718
Size: 1020 1698
SapI GCTCTTCn'nnn_
Cuts at: 0 1446 2718
Size: 1446 1272
SfcI C'TryA_G
Cuts at: 0 213 385 472 1021 1169 2718
Size: 213 172 87 549 148 1549
Fragments arranged by size:
1549 549 213 172 148 87
SfoI GGC'GCC
Cuts at: 0 741 2718
Size: 741 1977
SmlI C'TyrA_G
Cuts at: 0 824 1264 2718
Size: 824 440 1454
Fragments arranged by size:
1454 824 440
StuI AGG'CCT
Cuts at: 0 1417 2718
Size: 1417 1301
StyI C'CwwG_G
Cuts at: 0 36 996 2252 2381 2699 2718
Size: 36 960 1256 129 318 19
Fragments arranged by size:
1256 960 318 129 36 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2265 2718
Size: 2265 453
TatI w'GTAC_w
Cuts at: 0 860 2171 2718
Size: 860 1311 547
Fragments arranged by size:
1311 860 547
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 801 833 2178 2210 2718
Size: 801 32 1345 32 508
Fragments arranged by size:
1345 801 508 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 986 2718
Size: 986 1732
XhoI C'TCGA_G
Cuts at: 0 824 2718
Size: 824 1894
Enzymes that do cut:
AarI AccI AcuI AfeI AgeI AleI AlfI AlwNI
ApaI ApoI AvaI AvrII BanI BanII BbeI BbsI
BbvCI BciVI BglI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BstAPI BstXI BstYI BtgI BtsI
ClaI CspCI EaeI EarI EcoICRI Eco57MI EcoO109I FalI
HaeII Hin4I HincII KasI MslI MspA1I NarI NspI
PasI PfoI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuII SacI SapI SfcI SfoI SmlI
StuI StyI TaqII TatI TstI XcmI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AhdI AloI ApaLI
AscI AseI AsiSI BaeI BamHI BcgI BclI BfrBI
BglII BlpI BmtI BplI BsiWI BsmBI BspEI BsrGI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI DraI
DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI
MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PmeI PspXI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XmaI XmnI ZraI