Restriction map for
pF1KB8523
(Linear) (Six-base) MAPSORT of: KIBB8523.seq Check: 1478 from: 1 to: 2811
KIBB8523.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 776 2811
Size: 776 2035
AatII G_ACGT'C
Cuts at: 0 2324 2811
Size: 2324 487
AccI GT'mk_AC
Cuts at: 0 596 2623 2811
Size: 596 2027 188
Fragments arranged by size:
2027 596 188
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1970 2639 2811
Size: 1970 669 172
Fragments arranged by size:
1970 669 172
AfeI AGC'GCT
Cuts at: 0 2755 2811
Size: 2755 56
AflIII A'CryG_T
Cuts at: 0 719 1539 1564 1763 2219 2811
Size: 719 820 25 199 456 592
Fragments arranged by size:
820 719 592 456 199 25
AhdI GACnn_n'nnGTC
Cuts at: 0 46 2811
Size: 46 2765
AleI CACnn'nnGTG
Cuts at: 0 283 866 989 1124 1604 1739 1862 2272
Size: 283 583 123 135 480 135 123 410
Cuts at: 2272 2811
Size: 539
Fragments arranged by size:
583 539 480 410 283 135 135 123
123
AlwNI CAG_nnn'CTG
Cuts at: 0 843 2811
Size: 843 1968
ApaI G_GGCC'C
Cuts at: 0 462 2811
Size: 462 2349
ApaLI G'TGCA_C
Cuts at: 0 708 1074 1217 1340 2811
Size: 708 366 143 123 1471
Fragments arranged by size:
1471 708 366 143 123
ApoI r'AATT_y
Cuts at: 0 1878 2007 2811
Size: 1878 129 804
Fragments arranged by size:
1878 804 129
AvaI C'yCGr_G
Cuts at: 0 281 1448 2075 2811
Size: 281 1167 627 736
Fragments arranged by size:
1167 736 627 281
BamHI G'GATC_C
Cuts at: 0 1984 2811
Size: 1984 827
BanI G'GyrC_C
Cuts at: 0 397 2274 2811
Size: 397 1877 537
Fragments arranged by size:
1877 537 397
BanII G_rGCy'C
Cuts at: 0 462 632 898 2194 2313 2426 2811
Size: 462 170 266 1296 119 113 385
Fragments arranged by size:
1296 462 385 266 170 119 113
BbsI GAAGACnn'nnnn_
Cuts at: 0 639 1567 1935 2522 2535 2612 2811
Size: 639 928 368 587 13 77 199
Fragments arranged by size:
928 639 587 368 199 77 13
BbvCI CC'TCA_GC
Cuts at: 0 935 1796 1919 2811
Size: 935 861 123 892
Fragments arranged by size:
935 892 861 123
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 523 557 2811
Size: 523 34 2254
Fragments arranged by size:
2254 523 34
BglI GCCn_nnn'nGGC
Cuts at: 0 538 1166 1289 2811
Size: 538 628 123 1522
Fragments arranged by size:
1522 628 538 123
Bme1580I G_kGCm'C
Cuts at: 0 400 462 712 750 1078 1221 1344 2279
Size: 400 62 250 38 328 143 123 935
Cuts at: 2279 2811
Size: 532
Fragments arranged by size:
935 532 400 328 250 143 123 62
38
BmgBI CAC'GTC
Cuts at: 0 1565 2811
Size: 1565 1246
BmrI ACTGGGnnnn_n'
Cuts at: 0 416 2210 2811
Size: 416 1794 601
Fragments arranged by size:
1794 601 416
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2433 2811
Size: 2433 378
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 2074 2525 2811
Size: 160 1914 451 286
Fragments arranged by size:
1914 451 286 160
Bpu10I CC'TnA_GC
Cuts at: 0 545 935 1796 1919 2312 2811
Size: 545 390 861 123 393 499
Fragments arranged by size:
861 545 499 393 390 123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 1420 2473 2811
Size: 316 1104 1053 338
Fragments arranged by size:
1104 1053 338 316
BsaI GGTCTCn'nnnn_
Cuts at: 0 507 2811
Size: 507 2304
BsaAI yAC'GTr
Cuts at: 0 82 1542 1766 2811
Size: 82 1460 224 1045
Fragments arranged by size:
1460 1045 224 82
BsaHI Gr'CG_yC
Cuts at: 0 2321 2811
Size: 2321 490
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1529 1559 2811
Size: 1529 30 1252
Fragments arranged by size:
1529 1252 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 1276 2410 2811
Size: 43 1233 1134 401
Fragments arranged by size:
1233 1134 401 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 744 2811
Size: 744 2067
BsiHKAI G_wGCw'C
Cuts at: 0 712 1078 1221 1344 1408 1547 1590 2811
Size: 712 366 143 123 64 139 43 1221
Fragments arranged by size:
1221 712 366 143 139 123 64 43
BsmI GAATG_Cn'
Cuts at: 0 439 2811
Size: 439 2372
BsmBI CGTCTCn'nnnn_
Cuts at: 0 670 2328 2811
Size: 670 1658 483
Fragments arranged by size:
1658 670 483
Bsp1286I G_dGCh'C
Cuts at: 0 400 462 632 712 750 898 1078 1221
Size: 400 62 170 80 38 148 180 143
Cuts at: 1221 1344 1408 1547 1590 2194 2279 2313 2426
Size: 123 64 139 43 604 85 34 113
Cuts at: 2426 2811
Size: 385
Fragments arranged by size:
604 400 385 180 170 148 143 139
123 113 85 80 64 62 43 38
34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 776 1204 1327 1450 1696 2811
Size: 776 428 123 123 246 1115
Fragments arranged by size:
1115 776 428 246 123 123
BsrBI CCG'CTC
Cuts at: 0 75 2074 2811
Size: 75 1999 737
Fragments arranged by size:
1999 737 75
BssSI C'ACGA_G
Cuts at: 0 1120 2811
Size: 1120 1691
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1319 1943 2811
Size: 1319 624 868
Fragments arranged by size:
1319 868 624
BstXI CCAn_nnnn'nTGG
Cuts at: 0 585 2811
Size: 585 2226
BstYI r'GATC_y
Cuts at: 0 840 1510 1984 2811
Size: 840 670 474 827
Fragments arranged by size:
840 827 670 474
Bsu36I CC'TnA_GG
Cuts at: 0 469 518 1532 2811
Size: 469 49 1014 1279
Fragments arranged by size:
1279 1014 469 49
BtgI C'CryG_G
Cuts at: 0 392 484 527 851 984 1287 1353 1476
Size: 392 92 43 324 133 303 66 123
Cuts at: 1476 1599 1722 1845 2162 2811
Size: 123 123 123 317 649
Fragments arranged by size:
649 392 324 317 303 133 123 123
123 123 92 66 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 420 1568 2811
Size: 420 1148 1243
Fragments arranged by size:
1243 1148 420
BtsI GCAGTG_nn'
Cuts at: 0 204 1155 1632 2811
Size: 204 951 477 1179
Fragments arranged by size:
1179 951 477 204
ClaI AT'CG_AT
Cuts at: 0 1979 2811
Size: 1979 832
EaeI y'GGCC_r
Cuts at: 0 413 530 656 2811
Size: 413 117 126 2155
Fragments arranged by size:
2155 413 126 117
EarI CTCTTCn'nnn_
Cuts at: 0 940 1997 2446 2811
Size: 940 1057 449 365
Fragments arranged by size:
1057 940 449 365
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 1970 2074 2525 2639 2811
Size: 160 1810 104 451 114 172
Fragments arranged by size:
1810 451 172 160 114 104
EcoNI CCTnn'n_nnAGG
Cuts at: 0 342 934 1200 1692 2811
Size: 342 592 266 492 1119
Fragments arranged by size:
1119 592 492 342 266
EcoO109I rG'GnC_Cy
Cuts at: 0 458 1431 2258 2811
Size: 458 973 827 553
Fragments arranged by size:
973 827 553 458
EcoRI G'AATT_C
Cuts at: 0 1878 2811
Size: 1878 933
EcoRV GAT'ATC
Cuts at: 0 2593 2811
Size: 2593 218
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2457 2629 2811
Size: 2457 172 182
Fragments arranged by size:
2457 182 172
HaeII r_GCGC'y
Cuts at: 0 2757 2811
Size: 2757 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 32 64 369 401 1529 1561 1775 1807
Size: 32 32 305 32 1128 32 214 32
Cuts at: 1807 2308 2340 2811
Size: 501 32 471
Fragments arranged by size:
1128 501 471 305 214 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 204 1131 1254 1500 2811
Size: 204 927 123 246 1311
Fragments arranged by size:
1311 927 246 204 123
HindIII A'AGCT_T
Cuts at: 0 2632 2811
Size: 2632 179
HpaI GTT'AAC
Cuts at: 0 1131 2811
Size: 1131 1680
MfeI C'AATT_G
Cuts at: 0 1958 2811
Size: 1958 853
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2592 2811
Size: 2592 219
MscI TGG'CCA
Cuts at: 0 415 658 2811
Size: 415 243 2153
Fragments arranged by size:
2153 415 243
MslI CAynn'nnrTG
Cuts at: 0 215 283 583 743 866 989 1001 1124
Size: 215 68 300 160 123 123 12 123
Cuts at: 1124 1358 1481 1604 1616 1727 1739 1741 1764
Size: 234 123 123 12 111 12 2 23
Cuts at: 1764 1862 2272 2811
Size: 98 410 539
Fragments arranged by size:
539 410 300 234 215 160 123 123
123 123 123 111 98 68 23 12
12 12 2
MspA1I CmG'CkG
Cuts at: 0 1280 1368 2811
Size: 1280 88 1443
Fragments arranged by size:
1443 1280 88
NcoI C'CATG_G
Cuts at: 0 851 1353 1476 1722 1845 2811
Size: 851 502 123 246 123 966
Fragments arranged by size:
966 851 502 246 123 123
NspI r_CATG'y
Cuts at: 0 723 2223 2811
Size: 723 1500 588
Fragments arranged by size:
1500 723 588
PciI A'CATG_T
Cuts at: 0 719 2219 2811
Size: 719 1500 592
Fragments arranged by size:
1500 719 592
PfoI T'CCnGG_A
Cuts at: 0 836 2811
Size: 836 1975
PmlI CAC'GTG
Cuts at: 0 82 1542 1766 2811
Size: 82 1460 224 1045
Fragments arranged by size:
1460 1045 224 82
PpuMI rG'GwC_Cy
Cuts at: 0 1431 2811
Size: 1431 1380
PshAI GACnn'nnGTC
Cuts at: 0 266 2811
Size: 266 2545
PsiI TTA'TAA
Cuts at: 0 2707 2811
Size: 2707 104
PspOMI G'GGCC_C
Cuts at: 0 458 2811
Size: 458 2353
PspXI vC'TCGA_Gb
Cuts at: 0 2075 2811
Size: 2075 736
PstI C_TGCA'G
Cuts at: 0 787 1201 1324 1693 2811
Size: 787 414 123 369 1118
Fragments arranged by size:
1118 787 414 369 123
PvuII CAG'CTG
Cuts at: 0 1368 2811
Size: 1368 1443
SexAI A'CCwGG_T
Cuts at: 0 170 1964 2811
Size: 170 1794 847
Fragments arranged by size:
1794 847 170
SfcI C'TryA_G
Cuts at: 0 783 915 1197 1320 1689 1715 2811
Size: 783 132 282 123 369 26 1096
Fragments arranged by size:
1096 783 369 282 132 123 26
SmaI CCC'GGG
Cuts at: 0 1450 2811
Size: 1450 1361
SmlI C'TyrA_G
Cuts at: 0 331 1435 2075 2488 2811
Size: 331 1104 640 413 323
Fragments arranged by size:
1104 640 413 331 323
StyI C'CwwG_G
Cuts at: 0 851 1353 1476 1722 1845 2241 2345 2811
Size: 851 502 123 246 123 396 104 466
Fragments arranged by size:
851 502 466 396 246 123 123 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 223 2811
Size: 223 2588
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 936 968 1551 1583 2811
Size: 936 32 583 32 1228
Fragments arranged by size:
1228 936 583 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 643 1966 2811
Size: 643 1323 845
Fragments arranged by size:
1323 845 643
XbaI T'CTAG_A
Cuts at: 0 2511 2811
Size: 2511 300
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 408 916 2811
Size: 408 508 1895
Fragments arranged by size:
1895 508 408
XhoI C'TCGA_G
Cuts at: 0 2075 2811
Size: 2075 736
XmaI C'CCGG_G
Cuts at: 0 1448 2811
Size: 1448 1363
XmnI GAAnn'nnTTC
Cuts at: 0 322 2811
Size: 322 2489
ZraI GAC'GTC
Cuts at: 0 2322 2811
Size: 2322 489
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AleI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BglI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII HindIII HpaI MfeI MmeI MscI
MslI MspA1I NcoI NspI PciI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuII SexAI SfcI
SmaI SmlI StyI TaqII TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AlfI AloI AscI AseI
AsiSI AvrII BaeI BbeI BciVI BclI BfrBI BglII
BlpI BmtI BsaBI BsaWI BseYI BsiEI BsiWI BspEI
BspHI BsrDI BsrFI BsrGI BssHII BstBI BstEII BstZ17I
CspCI DraI DraIII DrdI EagI EciI EcoICRI FseI
FspI FspAI KasI KpnI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PmeI PpiI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI