Restriction map for
pF1KB8486
(Linear) (Six-base) MAPSORT of: KIBB8486.seq Check: 5550 from: 1 to: 2565
KIBB8486.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1195 2332 2565
Size: 1195 1137 233
Fragments arranged by size:
1195 1137 233
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 420 583 1572 2565
Size: 391 29 163 989 993
Fragments arranged by size:
993 989 391 163 29
AhdI GACnn_n'nnGTC
Cuts at: 0 795 2565
Size: 795 1770
AleI CACnn'nnGTG
Cuts at: 0 583 2565
Size: 583 1982
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 310 342 2565
Size: 310 32 2223
Fragments arranged by size:
2223 310 32
AlwNI CAG_nnn'CTG
Cuts at: 0 492 2565
Size: 492 2073
ApaI G_GGCC'C
Cuts at: 0 18 37 406 741 2565
Size: 18 19 369 335 1824
Fragments arranged by size:
1824 369 335 19 18
ApoI r'AATT_y
Cuts at: 0 297 1147 2565
Size: 297 850 1418
Fragments arranged by size:
1418 850 297
AvaI C'yCGr_G
Cuts at: 0 153 1359 2565
Size: 153 1206 1206
Fragments arranged by size:
1206 1206 153
AvrII C'CTAG_G
Cuts at: 0 1003 2565
Size: 1003 1562
BanI G'GyrC_C
Cuts at: 0 883 1113 1183 2565
Size: 883 230 70 1382
Fragments arranged by size:
1382 883 230 70
BanII G_rGCy'C
Cuts at: 0 18 37 406 741 850 860 1169 1451
Size: 18 19 369 335 109 10 309 282
Cuts at: 1451 1733 2565
Size: 282 832
Fragments arranged by size:
832 369 335 309 282 282 109 19
18 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 258 2205 2565
Size: 258 1947 360
Fragments arranged by size:
1947 360 258
BbvCI CC'TCA_GC
Cuts at: 0 2380 2565
Size: 2380 185
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 435 469 2565
Size: 435 34 2096
Fragments arranged by size:
2096 435 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1402 2565
Size: 1402 1163
BglI GCCn_nnn'nGGC
Cuts at: 0 2227 2565
Size: 2227 338
BlpI GC'TnA_GC
Cuts at: 0 766 2565
Size: 766 1799
Bme1580I G_kGCm'C
Cuts at: 0 18 37 406 741 1118 1186 1855 2565
Size: 18 19 369 335 377 68 669 710
Fragments arranged by size:
710 669 377 369 335 68 19 18
BmgBI CAC'GTC
Cuts at: 0 249 2565
Size: 249 2316
BmrI ACTGGGnnnn_n'
Cuts at: 0 1236 2253 2565
Size: 1236 1017 312
Fragments arranged by size:
1236 1017 312
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1853 2187 2565
Size: 1853 334 378
Fragments arranged by size:
1853 378 334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1183 1329 2164 2170 2565
Size: 1183 146 835 6 395
Fragments arranged by size:
1183 835 395 146 6
Bpu10I CC'TnA_GC
Cuts at: 0 844 2380 2565
Size: 844 1536 185
Fragments arranged by size:
1536 844 185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 1758 2065 2565
Size: 43 1715 307 500
Fragments arranged by size:
1715 500 307 43
BsaI GGTCTCn'nnnn_
Cuts at: 0 1959 2565
Size: 1959 606
BsaAI yAC'GTr
Cuts at: 0 1033 1203 1373 1484 2565
Size: 1033 170 170 111 1081
Fragments arranged by size:
1081 1033 170 170 111
BsaHI Gr'CG_yC
Cuts at: 0 752 1466 2201 2565
Size: 752 714 735 364
Fragments arranged by size:
752 735 714 364
BsaWI w'CCGG_w
Cuts at: 0 1394 2565
Size: 1394 1171
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 304 310 334 340 876 880 906 910
Size: 304 6 24 6 536 4 26 4
Cuts at: 910 1654 1684 2565
Size: 744 30 881
Fragments arranged by size:
881 744 536 304 30 26 24 6
6 4 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 184 514 538 909 2565
Size: 184 330 24 371 1656
Fragments arranged by size:
1656 371 330 184 24
BseYI C'CCAG_C
Cuts at: 0 161 207 1418 2246 2474 2565
Size: 161 46 1211 828 228 91
Fragments arranged by size:
1211 828 228 161 91 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 892 1080 1413 1587 2086 2565
Size: 637 255 188 333 174 499 479
Fragments arranged by size:
637 499 479 333 255 188 174
BsiEI CG_ry'CG
Cuts at: 0 12 2565
Size: 12 2553
BsiHKAI G_wGCw'C
Cuts at: 0 1451 2565
Size: 1451 1114
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2474 2565
Size: 2474 91
Bsp1286I G_dGCh'C
Cuts at: 0 18 37 406 741 850 860 1118 1169
Size: 18 19 369 335 109 10 258 51
Cuts at: 1169 1186 1451 1733 1855 2565
Size: 17 265 282 122 710
Fragments arranged by size:
710 369 335 282 265 258 122 109
51 19 18 17 10
BspEI T'CCGG_A
Cuts at: 0 1394 2565
Size: 1394 1171
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1166 1195 1875 2332 2565
Size: 1166 29 680 457 233
Fragments arranged by size:
1166 680 457 233 29
BsrBI CCG'CTC
Cuts at: 0 718 963 2565
Size: 718 245 1602
Fragments arranged by size:
1602 718 245
BsrDI GCAATG_nn'
Cuts at: 0 1638 2565
Size: 1638 927
BsrFI r'CCGG_y
Cuts at: 0 1312 1468 2157 2221 2461 2565
Size: 1312 156 689 64 240 104
Fragments arranged by size:
1312 689 240 156 104 64
BsrGI T'GTAC_A
Cuts at: 0 380 833 2513 2565
Size: 380 453 1680 52
Fragments arranged by size:
1680 453 380 52
BssHII G'CGCG_C
Cuts at: 0 553 2276 2565
Size: 553 1723 289
Fragments arranged by size:
1723 553 289
BssSI C'ACGA_G
Cuts at: 0 76 1827 1838 2565
Size: 76 1751 11 727
Fragments arranged by size:
1751 727 76 11
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2366 2565
Size: 2366 199
BstEII G'GTnAC_C
Cuts at: 0 576 2565
Size: 576 1989
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1950 2565
Size: 1950 615
BstYI r'GATC_y
Cuts at: 0 2300 2450 2565
Size: 2300 150 115
Fragments arranged by size:
2300 150 115
BtgI C'CryG_G
Cuts at: 0 547 599 660 851 2174 2565
Size: 547 52 61 191 1323 391
Fragments arranged by size:
1323 547 391 191 61 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 438 1457 2118 2565
Size: 438 1019 661 447
Fragments arranged by size:
1019 661 447 438
BtsI GCAGTG_nn'
Cuts at: 0 1633 1965 2017 2565
Size: 1633 332 52 548
Fragments arranged by size:
1633 548 332 52
ClaI AT'CG_AT
Cuts at: 0 1976 2565
Size: 1976 589
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 318 353 2565
Size: 318 35 2212
Fragments arranged by size:
2212 318 35
DraIII CAC_nnn'GTG
Cuts at: 0 495 2012 2565
Size: 495 1517 553
Fragments arranged by size:
1517 553 495
DrdI GACnn_nn'nnGTC
Cuts at: 0 253 2565
Size: 253 2312
EaeI y'GGCC_r
Cuts at: 0 347 518 1470 2565
Size: 347 171 952 1095
Fragments arranged by size:
1095 952 347 171
EarI CTCTTCn'nnn_
Cuts at: 0 1516 2565
Size: 1516 1049
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 41 2455 2463 2565
Size: 41 2414 8 102
Fragments arranged by size:
2414 102 41 8
EcoICRI GAG'CTC
Cuts at: 0 1449 2565
Size: 1449 1116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 420 583 1183 1329 1572 2164 2170
Size: 391 29 163 600 146 243 592 6
Cuts at: 2170 2565
Size: 395
Fragments arranged by size:
600 592 395 391 243 163 146 29
6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 496 2290 2565
Size: 496 1794 275
Fragments arranged by size:
1794 496 275
EcoO109I rG'GnC_Cy
Cuts at: 0 33 403 623 738 1238 2419 2565
Size: 33 370 220 115 500 1181 146
Fragments arranged by size:
1181 500 370 220 146 115 33
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1158 1527 2565
Size: 1158 369 1038
Fragments arranged by size:
1158 1038 369
HaeII r_GCGC'y
Cuts at: 0 162 2057 2565
Size: 162 1895 508
Fragments arranged by size:
1895 508 162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 742 774 781 813 1654 1686 2565
Size: 742 32 7 32 841 32 879
Fragments arranged by size:
879 841 742 32 32 32 7
HincII GTy'rAC
Cuts at: 0 123 2565
Size: 123 2442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 1685 2565
Size: 347 1338 880
Fragments arranged by size:
1338 880 347
MscI TGG'CCA
Cuts at: 0 349 2565
Size: 349 2216
MslI CAynn'nnrTG
Cuts at: 0 583 1111 1442 2565
Size: 583 528 331 1123
Fragments arranged by size:
1123 583 528 331
MspA1I CmG'CkG
Cuts at: 0 386 549 638 662 728 999 1016 1718
Size: 386 163 89 24 66 271 17 702
Cuts at: 1718 2384 2565
Size: 666 181
Fragments arranged by size:
702 666 386 271 181 163 89 66
24 17
NaeI GCC'GGC
Cuts at: 0 1470 2223 2565
Size: 1470 753 342
Fragments arranged by size:
1470 753 342
NcoI C'CATG_G
Cuts at: 0 851 2174 2565
Size: 851 1323 391
Fragments arranged by size:
1323 851 391
NgoMIV G'CCGG_C
Cuts at: 0 1468 2221 2565
Size: 1468 753 344
Fragments arranged by size:
1468 753 344
NspI r_CATG'y
Cuts at: 0 1132 1900 2565
Size: 1132 768 665
Fragments arranged by size:
1132 768 665
PasI CC'CwG_GG
Cuts at: 0 1041 2059 2565
Size: 1041 1018 506
Fragments arranged by size:
1041 1018 506
PflMI CCAn_nnn'nTGG
Cuts at: 0 867 2012 2565
Size: 867 1145 553
Fragments arranged by size:
1145 867 553
PmlI CAC'GTG
Cuts at: 0 1373 1484 2565
Size: 1373 111 1081
Fragments arranged by size:
1373 1081 111
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 2565
Size: 310 32 2223
Fragments arranged by size:
2223 310 32
PpuMI rG'GwC_Cy
Cuts at: 0 623 1238 2419 2565
Size: 623 615 1181 146
Fragments arranged by size:
1181 623 615 146
PshAI GACnn'nnGTC
Cuts at: 0 2424 2565
Size: 2424 141
PspOMI G'GGCC_C
Cuts at: 0 14 33 402 737 2565
Size: 14 19 369 335 1828
Fragments arranged by size:
1828 369 335 19 14
PstI C_TGCA'G
Cuts at: 0 643 1459 2565
Size: 643 816 1106
Fragments arranged by size:
1106 816 643
PvuI CG_AT'CG
Cuts at: 0 12 2565
Size: 12 2553
PvuII CAG'CTG
Cuts at: 0 638 999 1016 2565
Size: 638 361 17 1549
Fragments arranged by size:
1549 638 361 17
SacI G_AGCT'C
Cuts at: 0 1451 2565
Size: 1451 1114
SacII CC_GC'GG
Cuts at: 0 550 663 2565
Size: 550 113 1902
Fragments arranged by size:
1902 550 113
SanDI GG'GwC_CC
Cuts at: 0 2419 2565
Size: 2419 146
SexAI A'CCwGG_T
Cuts at: 0 491 821 2565
Size: 491 330 1744
Fragments arranged by size:
1744 491 330
SfcI C'TryA_G
Cuts at: 0 639 1455 1776 2565
Size: 639 816 321 789
Fragments arranged by size:
816 789 639 321
SmaI CCC'GGG
Cuts at: 0 155 1361 2565
Size: 155 1206 1204
Fragments arranged by size:
1206 1204 155
SmlI C'TyrA_G
Cuts at: 0 58 1737 2080 2565
Size: 58 1679 343 485
Fragments arranged by size:
1679 485 343 58
SnaBI TAC'GTA
Cuts at: 0 1033 2565
Size: 1033 1532
SphI G_CATG'C
Cuts at: 0 1900 2565
Size: 1900 665
StuI AGG'CCT
Cuts at: 0 1669 2565
Size: 1669 896
StyI C'CwwG_G
Cuts at: 0 851 1003 1193 1679 2174 2565
Size: 851 152 190 486 495 391
Fragments arranged by size:
851 495 486 391 190 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 153 1098 2272 2565
Size: 153 945 1174 293
Fragments arranged by size:
1174 945 293 153
TatI w'GTAC_w
Cuts at: 0 62 380 615 833 2165 2513 2565
Size: 62 318 235 218 1332 348 52
Fragments arranged by size:
1332 348 318 235 218 62 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 876 908 2565
Size: 876 32 1657
Fragments arranged by size:
1657 876 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1200 1630 2565
Size: 1200 430 935
Fragments arranged by size:
1200 935 430
XmaI C'CCGG_G
Cuts at: 0 153 1359 2565
Size: 153 1206 1206
Fragments arranged by size:
1206 1206 153
XmnI GAAnn'nnTTC
Cuts at: 0 43 265 1495 2565
Size: 43 222 1230 1070
Fragments arranged by size:
1230 1070 222 43
Enzymes that do cut:
AarI AcuI AhdI AleI AloI AlwNI ApaI ApoI
AvaI AvrII BanI BanII BbsI BbvCI BcgI BciVI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NspI PasI PflMI PmlI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII SacI SacII SanDI
SexAI SfcI SmaI SmlI SnaBI SphI StuI StyI
TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AlfI ApaLI AscI AseI AsiSI BaeI BamHI BbeI
BclI BfrBI BglII BmtI BsaBI BsiWI BsmI BspHI
BstBI BstZ17I Bsu36I DraI EagI EcoRI EcoRV FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
NarI NdeI NheI NotI NruI NsiI PacI PciI
PfoI PmeI PsiI PspXI PsrI RsrII SalI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SpeI SrfI
SspI SwaI Tth111I XbaI XhoI ZraI