Restriction map for
pF1KB8475
(Linear) (Six-base) MAPSORT of: KIBB8475.seq Check: 6306 from: 1 to: 2649
KIBB8475.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AatII G_ACGT'C
Cuts at: 0 1050 2649
Size: 1050 1599
AccI GT'mk_AC
Cuts at: 0 2271 2649
Size: 2271 378
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1819 2649
Size: 1819 830
AflII C'TTAA_G
Cuts at: 0 1735 2086 2649
Size: 1735 351 563
Fragments arranged by size:
1735 563 351
AflIII A'CryG_T
Cuts at: 0 1091 2274 2649
Size: 1091 1183 375
Fragments arranged by size:
1183 1091 375
AleI CACnn'nnGTG
Cuts at: 0 512 1961 2649
Size: 512 1449 688
Fragments arranged by size:
1449 688 512
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1670 1702 2649
Size: 1670 32 947
Fragments arranged by size:
1670 947 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2305 2649
Size: 2305 344
ApoI r'AATT_y
Cuts at: 0 250 274 583 700 1856 2649
Size: 250 24 309 117 1156 793
Fragments arranged by size:
1156 793 309 250 117 24
AseI AT'TA_AT
Cuts at: 0 1820 2649
Size: 1820 829
AvaI C'yCGr_G
Cuts at: 0 941 2649
Size: 941 1708
BanII G_rGCy'C
Cuts at: 0 992 2457 2469 2649
Size: 992 1465 12 180
Fragments arranged by size:
1465 992 180 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 1214 1472 2649
Size: 1214 258 1177
Fragments arranged by size:
1214 1177 258
BciVI GTATCCnnnnn_n'
Cuts at: 0 2575 2649
Size: 2575 74
BglII A'GATC_T
Cuts at: 0 864 2629 2649
Size: 864 1765 20
Fragments arranged by size:
1765 864 20
BlpI GC'TnA_GC
Cuts at: 0 1070 1127 2649
Size: 1070 57 1522
Fragments arranged by size:
1522 1070 57
BmgBI CAC'GTC
Cuts at: 0 1092 2649
Size: 1092 1557
BmrI ACTGGGnnnn_n'
Cuts at: 0 41 1703 2649
Size: 41 1662 946
Fragments arranged by size:
1662 946 41
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 968 1223 2649
Size: 968 255 1426
Fragments arranged by size:
1426 968 255
Bpu10I CC'TnA_GC
Cuts at: 0 986 2649
Size: 986 1663
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 976 1615 2649
Size: 976 639 1034
Fragments arranged by size:
1034 976 639
BsaBI GATnn'nnATC
Cuts at: 0 1757 2649
Size: 1757 892
BsaHI Gr'CG_yC
Cuts at: 0 1047 2649
Size: 1047 1602
BsaWI w'CCGG_w
Cuts at: 0 270 2649
Size: 270 2379
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1533 1563 1964 1994 2649
Size: 1533 30 401 30 655
Fragments arranged by size:
1533 655 401 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1408 1985 2649
Size: 1408 577 664
Fragments arranged by size:
1408 664 577
BseYI C'CCAG_C
Cuts at: 0 1890 2076 2649
Size: 1890 186 573
Fragments arranged by size:
1890 573 186
BsiHKAI G_wGCw'C
Cuts at: 0 992 1958 2457 2649
Size: 992 966 499 192
Fragments arranged by size:
992 966 499 192
BsmI GAATG_Cn'
Cuts at: 0 926 1131 2425 2649
Size: 926 205 1294 224
Fragments arranged by size:
1294 926 224 205
Bsp1286I G_dGCh'C
Cuts at: 0 992 1958 2457 2469 2649
Size: 992 966 499 12 180
Fragments arranged by size:
992 966 499 180 12
BspEI T'CCGG_A
Cuts at: 0 270 2649
Size: 270 2379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2551 2649
Size: 2551 98
BsrDI GCAATG_nn'
Cuts at: 0 2520 2649
Size: 2520 129
BsrGI T'GTAC_A
Cuts at: 0 424 568 1437 2649
Size: 424 144 869 1212
Fragments arranged by size:
1212 869 424 144
BstBI TT'CG_AA
Cuts at: 0 525 2460 2649
Size: 525 1935 189
Fragments arranged by size:
1935 525 189
BstEII G'GTnAC_C
Cuts at: 0 1703 2649
Size: 1703 946
BstYI r'GATC_y
Cuts at: 0 109 864 2629 2649
Size: 109 755 1765 20
Fragments arranged by size:
1765 755 109 20
BtgI C'CryG_G
Cuts at: 0 2481 2649
Size: 2481 168
DraI TTT'AAA
Cuts at: 0 705 1055 1727 2649
Size: 705 350 672 922
Fragments arranged by size:
922 705 672 350
DrdI GACnn_nn'nnGTC
Cuts at: 0 1092 2649
Size: 1092 1557
EaeI y'GGCC_r
Cuts at: 0 2061 2649
Size: 2061 588
EarI CTCTTCn'nnn_
Cuts at: 0 60 125 752 842 1349 1993 1999 2462
Size: 60 65 627 90 507 644 6 463
Cuts at: 2462 2649
Size: 187
Fragments arranged by size:
644 627 507 463 187 90 65 60
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 669 2649
Size: 669 1980
EcoICRI GAG'CTC
Cuts at: 0 990 2455 2649
Size: 990 1465 194
Fragments arranged by size:
1465 990 194
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 968 1223 1819 2649
Size: 968 255 596 830
Fragments arranged by size:
968 830 596 255
EcoNI CCTnn'n_nnAGG
Cuts at: 0 331 2649
Size: 331 2318
EcoRV GAT'ATC
Cuts at: 0 1161 1428 2649
Size: 1161 267 1221
Fragments arranged by size:
1221 1161 267
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 114 208 1081 2522 2649
Size: 114 94 873 1441 127
Fragments arranged by size:
1441 873 127 114 94
HaeII r_GCGC'y
Cuts at: 0 1119 2649
Size: 1119 1530
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2253 2285 2320 2352 2649
Size: 2253 32 35 32 297
Fragments arranged by size:
2253 297 35 32 32
HincII GTy'rAC
Cuts at: 0 2272 2649
Size: 2272 377
HindIII A'AGCT_T
Cuts at: 0 641 1917 2649
Size: 641 1276 732
Fragments arranged by size:
1276 732 641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1190 1713 2649
Size: 1190 523 936
Fragments arranged by size:
1190 936 523
MscI TGG'CCA
Cuts at: 0 2063 2649
Size: 2063 586
MslI CAynn'nnrTG
Cuts at: 0 217 405 512 931 1820 1961 2649
Size: 217 188 107 419 889 141 688
Fragments arranged by size:
889 688 419 217 188 141 107
MspA1I CmG'CkG
Cuts at: 0 1126 1750 1929 2649
Size: 1126 624 179 720
Fragments arranged by size:
1126 720 624 179
NdeI CA'TA_TG
Cuts at: 0 120 2649
Size: 120 2529
NspI r_CATG'y
Cuts at: 0 2278 2527 2649
Size: 2278 249 122
Fragments arranged by size:
2278 249 122
PciI A'CATG_T
Cuts at: 0 2274 2649
Size: 2274 375
PflMI CCAn_nnn'nTGG
Cuts at: 0 510 1657 2649
Size: 510 1147 992
Fragments arranged by size:
1147 992 510
PshAI GACnn'nnGTC
Cuts at: 0 499 2649
Size: 499 2150
PsiI TTA'TAA
Cuts at: 0 196 638 2649
Size: 196 442 2011
Fragments arranged by size:
2011 442 196
PvuII CAG'CTG
Cuts at: 0 1126 1750 2649
Size: 1126 624 899
Fragments arranged by size:
1126 899 624
SacI G_AGCT'C
Cuts at: 0 992 2457 2649
Size: 992 1465 192
Fragments arranged by size:
1465 992 192
SalI G'TCGA_C
Cuts at: 0 2270 2649
Size: 2270 379
SapI GCTCTTCn'nnn_
Cuts at: 0 2462 2649
Size: 2462 187
ScaI AGT'ACT
Cuts at: 0 446 2649
Size: 446 2203
SmlI C'TyrA_G
Cuts at: 0 991 1630 1735 2086 2649
Size: 991 639 105 351 563
Fragments arranged by size:
991 639 563 351 105
SpeI A'CTAG_T
Cuts at: 0 480 2649
Size: 480 2169
SspI AAT'ATT
Cuts at: 0 805 1522 2649
Size: 805 717 1127
Fragments arranged by size:
1127 805 717
StyI C'CwwG_G
Cuts at: 0 89 2649
Size: 89 2560
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 502 2649
Size: 502 2147
TatI w'GTAC_w
Cuts at: 0 424 444 568 1213 1437 1695 1923 2368
Size: 424 20 124 645 224 258 228 445
Cuts at: 2368 2649
Size: 281
Fragments arranged by size:
645 445 424 281 258 228 224 124
20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1227 1259 2649
Size: 1227 32 1390
Fragments arranged by size:
1390 1227 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 511 2249 2649
Size: 511 1738 400
Fragments arranged by size:
1738 511 400
XmnI GAAnn'nnTTC
Cuts at: 0 2505 2649
Size: 2505 144
ZraI GAC'GTC
Cuts at: 0 1048 2649
Size: 1048 1601
Enzymes that do cut:
AatII AccI AcuI AflII AflIII AleI AloI AlwNI
ApoI AseI AvaI BanII BbsI BciVI BglII BlpI
BmgBI BmrI BpmI Bpu10I BpuEI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI BspMI
BsrDI BsrGI BstBI BstEII BstYI BtgI DraI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoRV FalI
HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I
NdeI NspI PciI PflMI PshAI PsiI PvuII SacI
SalI SapI ScaI SmlI SpeI SspI StyI TaqII
TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AgeI AhdI AlfI ApaI
ApaLI AscI AsiSI AvrII BaeI BamHI BanI BbeI
BbvCI BcgI BclI BfrBI BglI Bme1580I BmtI BplI
BsaI BsaAI BsgI BsiEI BsiWI BsmBI BspHI BsrBI
BsrFI BssHII BssSI BstAPI BstXI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraIII EagI EcoO109I EcoRI FseI
FspI FspAI HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI PacI
PasI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SanDI SbfI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI
SrfI StuI SwaI Tth111I XbaI XhoI XmaI