Restriction map for
pF1KB8467
(Linear) (Six-base) MAPSORT of: KIBB8467.seq Check: 9260 from: 1 to: 1347
KIBB8467.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AccI GT'mk_AC
Cuts at: 0 1031 1347
Size: 1031 316
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 71 103 1347
Size: 71 32 1244
Fragments arranged by size:
1244 71 32
AlwNI CAG_nnn'CTG
Cuts at: 0 881 1347
Size: 881 466
ApaI G_GGCC'C
Cuts at: 0 741 1167 1347
Size: 741 426 180
Fragments arranged by size:
741 426 180
ApoI r'AATT_y
Cuts at: 0 1249 1342 1347
Size: 1249 93 5
Fragments arranged by size:
1249 93 5
AvaI C'yCGr_G
Cuts at: 0 188 311 918 1090 1347
Size: 188 123 607 172 257
Fragments arranged by size:
607 257 188 172 123
BanI G'GyrC_C
Cuts at: 0 777 969 1347
Size: 777 192 378
Fragments arranged by size:
777 378 192
BanII G_rGCy'C
Cuts at: 0 68 551 741 893 1167 1347
Size: 68 483 190 152 274 180
Fragments arranged by size:
483 274 190 180 152 68
BbsI GAAGACnn'nnnn_
Cuts at: 0 1274 1347
Size: 1274 73
BclI T'GATC_A
Cuts at: 0 275 1347
Size: 275 1072
BfrBI ATG'CAT
Cuts at: 0 11 1347
Size: 11 1336
BglI GCCn_nnn'nGGC
Cuts at: 0 398 1204 1347
Size: 398 806 143
Fragments arranged by size:
806 398 143
BglII A'GATC_T
Cuts at: 0 204 1347
Size: 204 1143
BlpI GC'TnA_GC
Cuts at: 0 1132 1347
Size: 1132 215
Bme1580I G_kGCm'C
Cuts at: 0 188 433 741 782 974 1167 1347
Size: 188 245 308 41 192 193 180
Fragments arranged by size:
308 245 193 192 188 180 41
BmgBI CAC'GTC
Cuts at: 0 966 1347
Size: 966 381
BmtI G_CTAG'C
Cuts at: 0 122 1347
Size: 122 1225
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 520 1303 1347
Size: 229 291 783 44
Fragments arranged by size:
783 291 229 44
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 1347
Size: 98 1249
BsaI GGTCTCn'nnnn_
Cuts at: 0 534 1091 1347
Size: 534 557 256
Fragments arranged by size:
557 534 256
BsaAI yAC'GTr
Cuts at: 0 24 1347
Size: 24 1323
BseYI C'CCAG_C
Cuts at: 0 59 516 1347
Size: 59 457 831
Fragments arranged by size:
831 457 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1132 1347
Size: 1132 215
BsiEI CG_ry'CG
Cuts at: 0 1082 1347
Size: 1082 265
BsiHKAI G_wGCw'C
Cuts at: 0 68 1347
Size: 68 1279
BsmI GAATG_Cn'
Cuts at: 0 13 1347
Size: 13 1334
Bsp1286I G_dGCh'C
Cuts at: 0 68 188 433 551 741 782 893 974
Size: 68 120 245 118 190 41 111 81
Cuts at: 974 1167 1347
Size: 193 180
Fragments arranged by size:
245 193 190 180 120 118 111 81
68 41
BspHI T'CATG_A
Cuts at: 0 197 1347
Size: 197 1150
BsrDI GCAATG_nn'
Cuts at: 0 57 177 1148 1347
Size: 57 120 971 199
Fragments arranged by size:
971 199 120 57
BstAPI GCAn_nnn'nTGC
Cuts at: 0 56 1347
Size: 56 1291
BstEII G'GTnAC_C
Cuts at: 0 807 1347
Size: 807 540
BstXI CCAn_nnnn'nTGG
Cuts at: 0 421 1347
Size: 421 926
BstYI r'GATC_y
Cuts at: 0 204 1347
Size: 204 1143
BtgI C'CryG_G
Cuts at: 0 1082 1142 1347
Size: 1082 60 205
Fragments arranged by size:
1082 205 60
BtsI GCAGTG_nn'
Cuts at: 0 545 713 1347
Size: 545 168 634
Fragments arranged by size:
634 545 168
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1131 1166 1347
Size: 1131 35 181
Fragments arranged by size:
1131 181 35
EaeI y'GGCC_r
Cuts at: 0 863 1103 1145 1347
Size: 863 240 42 202
Fragments arranged by size:
863 240 202 42
EarI CTCTTCn'nnn_
Cuts at: 0 73 262 1347
Size: 73 189 1085
Fragments arranged by size:
1085 189 73
EcoICRI GAG'CTC
Cuts at: 0 66 1347
Size: 66 1281
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 520 1303 1347
Size: 229 291 783 44
Fragments arranged by size:
783 291 229 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1105 1285 1347
Size: 1105 180 62
Fragments arranged by size:
1105 180 62
EcoO109I rG'GnC_Cy
Cuts at: 0 737 738 1347
Size: 737 1 609
Fragments arranged by size:
737 609 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1224 1347
Size: 1224 123
HaeII r_GCGC'y
Cuts at: 0 663 1347
Size: 663 684
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 124 156 370 402 1347
Size: 124 32 214 32 945
Fragments arranged by size:
945 214 124 32 32
MscI TGG'CCA
Cuts at: 0 1105 1347
Size: 1105 242
MslI CAynn'nnrTG
Cuts at: 0 1220 1347
Size: 1220 127
MspA1I CmG'CkG
Cuts at: 0 273 804 1116 1347
Size: 273 531 312 231
Fragments arranged by size:
531 312 273 231
NheI G'CTAG_C
Cuts at: 0 118 1347
Size: 118 1229
NsiI A_TGCA'T
Cuts at: 0 13 1347
Size: 13 1334
NspI r_CATG'y
Cuts at: 0 184 951 1131 1347
Size: 184 767 180 216
Fragments arranged by size:
767 216 184 180
PmlI CAC'GTG
Cuts at: 0 24 1347
Size: 24 1323
PshAI GACnn'nnGTC
Cuts at: 0 1037 1084 1347
Size: 1037 47 263
Fragments arranged by size:
1037 263 47
PspOMI G'GGCC_C
Cuts at: 0 737 1163 1347
Size: 737 426 184
Fragments arranged by size:
737 426 184
PstI C_TGCA'G
Cuts at: 0 398 540 1347
Size: 398 142 807
Fragments arranged by size:
807 398 142
PvuII CAG'CTG
Cuts at: 0 273 1347
Size: 273 1074
SacI G_AGCT'C
Cuts at: 0 68 1347
Size: 68 1279
SapI GCTCTTCn'nnn_
Cuts at: 0 73 262 1347
Size: 73 189 1085
Fragments arranged by size:
1085 189 73
SbfI CC_TGCA'GG
Cuts at: 0 398 1347
Size: 398 949
SexAI A'CCwGG_T
Cuts at: 0 347 773 1347
Size: 347 426 574
Fragments arranged by size:
574 426 347
SfcI C'TryA_G
Cuts at: 0 218 394 536 1347
Size: 218 176 142 811
Fragments arranged by size:
811 218 176 142
SmaI CCC'GGG
Cuts at: 0 190 313 1092 1347
Size: 190 123 779 255
Fragments arranged by size:
779 255 190 123
SmlI C'TyrA_G
Cuts at: 0 113 1347
Size: 113 1234
SphI G_CATG'C
Cuts at: 0 951 1131 1347
Size: 951 180 216
Fragments arranged by size:
951 216 180
StuI AGG'CCT
Cuts at: 0 1159 1347
Size: 1159 188
StyI C'CwwG_G
Cuts at: 0 974 1106 1347
Size: 974 132 241
Fragments arranged by size:
974 241 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 586 1347
Size: 586 761
TatI w'GTAC_w
Cuts at: 0 932 1347
Size: 932 415
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 421 900 1347
Size: 421 479 447
Fragments arranged by size:
479 447 421
XmaI C'CCGG_G
Cuts at: 0 188 311 1090 1347
Size: 188 123 779 257
Fragments arranged by size:
779 257 188 123
Enzymes that do cut:
AccI AloI AlwNI ApaI ApoI AvaI BanI BanII
BbsI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmtI BpmI BpuEI BsaI BsaAI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BsrDI BstAPI BstEII BstXI
BstYI BtgI BtsI CspCI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I MscI MslI MspA1I
NheI NsiI NspI PmlI PshAI PspOMI PstI PvuII
SacI SapI SbfI SexAI SfcI SmaI SmlI SphI
StuI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BmrI
BplI Bpu10I BsaBI BsaHI BsaWI BsaXI BseRI BsiWI
BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI
EagI EciI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NotI NruI PacI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI ScaI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
TstI Tth111I XbaI XhoI XmnI ZraI