Restriction map for
pF1KB8462
(Linear) (Six-base) MAPSORT of: KIBB8462.seq Check: 5681 from: 1 to: 1128
KIBB8462.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
Acc65I G'GTAC_C
Cuts at: 0 852 1128
Size: 852 276
AflIII A'CryG_T
Cuts at: 0 561 1128
Size: 561 567
AgeI A'CCGG_T
Cuts at: 0 160 1128
Size: 160 968
AlwNI CAG_nnn'CTG
Cuts at: 0 991 1128
Size: 991 137
ApoI r'AATT_y
Cuts at: 0 110 306 1128
Size: 110 196 822
Fragments arranged by size:
822 196 110
AvrII C'CTAG_G
Cuts at: 0 684 1128
Size: 684 444
BanI G'GyrC_C
Cuts at: 0 129 514 852 1128
Size: 129 385 338 276
Fragments arranged by size:
385 338 276 129
BanII G_rGCy'C
Cuts at: 0 807 1128
Size: 807 321
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 318 352 460 494 1128
Size: 318 34 108 34 634
Fragments arranged by size:
634 318 108 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 784 1128
Size: 784 344
Bme1580I G_kGCm'C
Cuts at: 0 707 1128
Size: 707 421
BmrI ACTGGGnnnn_n'
Cuts at: 0 613 1128
Size: 613 515
BmtI G_CTAG'C
Cuts at: 0 1067 1128
Size: 1067 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 1128
Size: 125 1003
BsaAI yAC'GTr
Cuts at: 0 562 1128
Size: 562 566
BsaWI w'CCGG_w
Cuts at: 0 160 642 1128
Size: 160 482 486
Fragments arranged by size:
486 482 160
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 33 63 1128
Size: 33 30 1065
Fragments arranged by size:
1065 33 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 815 1128
Size: 815 313
BseYI C'CCAG_C
Cuts at: 0 226 1128
Size: 226 902
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 860 1128
Size: 860 268
BsiHKAI G_wGCw'C
Cuts at: 0 267 1128
Size: 267 861
Bsp1286I G_dGCh'C
Cuts at: 0 267 707 807 1128
Size: 267 440 100 321
Fragments arranged by size:
440 321 267 100
BspEI T'CCGG_A
Cuts at: 0 642 1128
Size: 642 486
BsrBI CCG'CTC
Cuts at: 0 850 1128
Size: 850 278
BsrDI GCAATG_nn'
Cuts at: 0 65 696 1128
Size: 65 631 432
Fragments arranged by size:
631 432 65
BsrFI r'CCGG_y
Cuts at: 0 151 160 1128
Size: 151 9 968
Fragments arranged by size:
968 151 9
BsrGI T'GTAC_A
Cuts at: 0 1025 1128
Size: 1025 103
BstAPI GCAn_nnn'nTGC
Cuts at: 0 955 991 1128
Size: 955 36 137
Fragments arranged by size:
955 137 36
BstEII G'GTnAC_C
Cuts at: 0 28 1128
Size: 28 1100
BstYI r'GATC_y
Cuts at: 0 241 1128
Size: 241 887
BtgI C'CryG_G
Cuts at: 0 201 518 782 1128
Size: 201 317 264 346
Fragments arranged by size:
346 317 264 201
BtsI GCAGTG_nn'
Cuts at: 0 707 1128
Size: 707 421
EaeI y'GGCC_r
Cuts at: 0 153 276 1128
Size: 153 123 852
Fragments arranged by size:
852 153 123
EarI CTCTTCn'nnn_
Cuts at: 0 711 1128
Size: 711 417
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 840 1128
Size: 840 288
EcoO109I rG'GnC_Cy
Cuts at: 0 369 1128
Size: 369 759
EcoRI G'AATT_C
Cuts at: 0 110 1128
Size: 110 1018
HaeII r_GCGC'y
Cuts at: 0 1082 1128
Size: 1082 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 33 65 304 336 710 742 1128
Size: 33 32 239 32 374 32 386
Fragments arranged by size:
386 374 239 33 32 32 32
KpnI G_GTAC'C
Cuts at: 0 856 1128
Size: 856 272
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 606 883 1128
Size: 586 20 277 245
Fragments arranged by size:
586 277 245 20
MspA1I CmG'CkG
Cuts at: 0 979 991 1128
Size: 979 12 137
Fragments arranged by size:
979 137 12
NaeI GCC'GGC
Cuts at: 0 153 1128
Size: 153 975
NdeI CA'TA_TG
Cuts at: 0 594 1128
Size: 594 534
NgoMIV G'CCGG_C
Cuts at: 0 151 1128
Size: 151 977
NheI G'CTAG_C
Cuts at: 0 1063 1128
Size: 1063 65
NspI r_CATG'y
Cuts at: 0 343 1128
Size: 343 785
PflMI CCAn_nnn'nTGG
Cuts at: 0 625 1128
Size: 625 503
PfoI T'CCnGG_A
Cuts at: 0 365 1128
Size: 365 763
PmlI CAC'GTG
Cuts at: 0 562 1128
Size: 562 566
PpuMI rG'GwC_Cy
Cuts at: 0 369 1128
Size: 369 759
PstI C_TGCA'G
Cuts at: 0 777 987 996 1128
Size: 777 210 9 132
Fragments arranged by size:
777 210 132 9
SanDI GG'GwC_CC
Cuts at: 0 369 1128
Size: 369 759
SfcI C'TryA_G
Cuts at: 0 554 773 939 983 992 1107 1128
Size: 554 219 166 44 9 115 21
Fragments arranged by size:
554 219 166 115 44 21 9
SmlI C'TyrA_G
Cuts at: 0 140 1128
Size: 140 988
SspI AAT'ATT
Cuts at: 0 351 1128
Size: 351 777
StyI C'CwwG_G
Cuts at: 0 684 1128
Size: 684 444
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 240 1128
Size: 240 888
TatI w'GTAC_w
Cuts at: 0 1025 1128
Size: 1025 103
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 723 755 1128
Size: 723 32 373
Fragments arranged by size:
723 373 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 579 1128
Size: 579 549
Enzymes that do cut:
Acc65I AflIII AgeI AlwNI ApoI AvrII BanI BanII
BcgI BglI Bme1580I BmrI BmtI BpuEI BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BsrBI
BsrDI BsrFI BsrGI BstAPI BstEII BstYI BtgI BtsI
EaeI EarI EciI EcoO109I EcoRI HaeII Hin4I KpnI
MmeI MspA1I NaeI NdeI NgoMIV NheI NspI PflMI
PfoI PmlI PpuMI PstI SanDI SfcI SmlI SspI
StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI BaeI BamHI BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI BmgBI BplI BpmI Bpu10I BsaI
BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BspHI BspMI
BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EcoICRI Eco57MI EcoNI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI MfeI MluI MscI MslI NarI NcoI NotI
NruI NsiI PacI PasI PciI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI Tth111I XbaI XhoI XmaI XmnI ZraI