Restriction map for
pF1KB8459
(Linear) (Six-base) MAPSORT of: KIBB8459.seq Check: 1774 from: 1 to: 1404
KIBB8459.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
Acc65I G'GTAC_C
Cuts at: 0 195 1404
Size: 195 1209
AclI AA'CG_TT
Cuts at: 0 703 1220 1268 1404
Size: 703 517 48 136
Fragments arranged by size:
703 517 136 48
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 749 1404
Size: 83 666 655
Fragments arranged by size:
666 655 83
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1228 1404
Size: 1228 176
ApoI r'AATT_y
Cuts at: 0 618 1379 1404
Size: 618 761 25
Fragments arranged by size:
761 618 25
BanI G'GyrC_C
Cuts at: 0 195 1404
Size: 195 1209
BanII G_rGCy'C
Cuts at: 0 850 1404
Size: 850 554
BbsI GAAGACnn'nnnn_
Cuts at: 0 1174 1404
Size: 1174 230
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1294 1328 1404
Size: 1294 34 76
Fragments arranged by size:
1294 76 34
Bme1580I G_kGCm'C
Cuts at: 0 291 1404
Size: 291 1113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 224 260 882 1404
Size: 182 42 36 622 522
Fragments arranged by size:
622 522 182 42 36
BsaAI yAC'GTr
Cuts at: 0 522 1404
Size: 522 882
BsaBI GATnn'nnATC
Cuts at: 0 150 1404
Size: 150 1254
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 645 675 1404
Size: 645 30 729
Fragments arranged by size:
729 645 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 179 221 411 414 1404
Size: 179 42 190 3 990
Fragments arranged by size:
990 190 179 42 3
BsmI GAATG_Cn'
Cuts at: 0 1000 1317 1404
Size: 1000 317 87
Fragments arranged by size:
1000 317 87
Bsp1286I G_dGCh'C
Cuts at: 0 291 850 1404
Size: 291 559 554
Fragments arranged by size:
559 554 291
BstYI r'GATC_y
Cuts at: 0 321 1404
Size: 321 1083
BtgI C'CryG_G
Cuts at: 0 83 1404
Size: 83 1321
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 354 389 1404
Size: 354 35 1015
Fragments arranged by size:
1015 354 35
DraI TTT'AAA
Cuts at: 0 174 183 561 1119 1148 1404
Size: 174 9 378 558 29 256
Fragments arranged by size:
558 378 256 174 29 9
EaeI y'GGCC_r
Cuts at: 0 2 86 479 1404
Size: 2 84 393 925
Fragments arranged by size:
925 393 84 2
EarI CTCTTCn'nnn_
Cuts at: 0 59 945 1010 1404
Size: 59 886 65 394
Fragments arranged by size:
886 394 65 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 182 224 260 749 882 1404
Size: 83 99 42 36 489 133 522
Fragments arranged by size:
522 489 133 99 83 42 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 203 254 1404
Size: 203 51 1150
Fragments arranged by size:
1150 203 51
EcoRI G'AATT_C
Cuts at: 0 618 1404
Size: 618 786
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 351 1404
Size: 351 1053
HaeII r_GCGC'y
Cuts at: 0 239 1404
Size: 239 1165
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 745 769 777 801 1404
Size: 745 24 8 24 603
Fragments arranged by size:
745 603 24 24 8
HincII GTy'rAC
Cuts at: 0 701 1404
Size: 701 703
HindIII A'AGCT_T
Cuts at: 0 446 1404
Size: 446 958
KpnI G_GTAC'C
Cuts at: 0 199 1404
Size: 199 1205
MfeI C'AATT_G
Cuts at: 0 1298 1404
Size: 1298 106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 435 1404
Size: 123 312 969
Fragments arranged by size:
969 312 123
MscI TGG'CCA
Cuts at: 0 4 88 1404
Size: 4 84 1316
Fragments arranged by size:
1316 84 4
MspA1I CmG'CkG
Cuts at: 0 316 649 1404
Size: 316 333 755
Fragments arranged by size:
755 333 316
NcoI C'CATG_G
Cuts at: 0 83 1404
Size: 83 1321
PmlI CAC'GTG
Cuts at: 0 522 1404
Size: 522 882
PvuII CAG'CTG
Cuts at: 0 649 1404
Size: 649 755
SfcI C'TryA_G
Cuts at: 0 1154 1404
Size: 1154 250
SspI AAT'ATT
Cuts at: 0 577 1404
Size: 577 827
StyI C'CwwG_G
Cuts at: 0 83 326 368 1404
Size: 83 243 42 1036
Fragments arranged by size:
1036 243 83 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1047 1248 1404
Size: 1047 201 156
Fragments arranged by size:
1047 201 156
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 960 1404
Size: 960 444
Enzymes that do cut:
Acc65I AclI AcuI AlfI ApoI BanI BanII BbsI
BcgI Bme1580I BpmI BsaAI BsaBI BsaXI BseRI BsmI
Bsp1286I BstYI BtgI CspCI DraI EaeI EarI Eco57MI
EcoNI EcoRI FalI HaeII Hin4I HincII HindIII KpnI
MfeI MmeI MscI MspA1I NcoI PmlI PvuII SfcI
SspI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI AfeI AflII AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI BtsI ClaI DraIII DrdI EagI
EciI EcoICRI EcoO109I EcoRV FseI FspI FspAI HpaI
KasI MluI MslI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI StuI SwaI
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI