(Linear) (Six-base) MAPSORT of: KIBB8455.seq  Check: 6900  from: 1  to: 345



KIBB8455.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

AccI GT'mk_AC

Cuts at:      0     284     345 
   Size:        284      61
 
AleI CACnn'nnGTG

Cuts at:      0      25     345 
   Size:         25     320
 
AlwNI CAG_nnn'CTG

Cuts at:      0      78     345 
   Size:         78     267
 
ApaLI G'TGCA_C

Cuts at:      0     118     345 
   Size:        118     227
 
BanII G_rGCy'C

Cuts at:      0      68     345 
   Size:         68     277
 
Bme1580I G_kGCm'C

Cuts at:      0      32     122     345 
   Size:         32      90     223

  Fragments arranged by size: 

                 223      90      32
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      99     345 
   Size:         99     246
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     141     345 
   Size:        141     204
 
BsaAI yAC'GTr

Cuts at:      0     123     345 
   Size:        123     222
 
BsaBI GATnn'nnATC

Cuts at:      0      10     345 
   Size:         10     335
 
BseYI C'CCAG_C

Cuts at:      0      71     345 
   Size:         71     274
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     345 
   Size:        169     176
 
BsiHKAI G_wGCw'C

Cuts at:      0      68     122     345 
   Size:         68      54     223

  Fragments arranged by size: 

                 223      68      54
 
Bsp1286I G_dGCh'C

Cuts at:      0      32      68     122     345 
   Size:         32      36      54     223

  Fragments arranged by size: 

                 223      54      36      32
 
BsrBI CCG'CTC

Cuts at:      0     221     345 
   Size:        221     124
 
BsrFI r'CCGG_y

Cuts at:      0      91     345 
   Size:         91     254
 
BssHII G'CGCG_C

Cuts at:      0     170     209     345 
   Size:        170      39     136

  Fragments arranged by size: 

                 170     136      39
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      96     345 
   Size:         96     249
 
EaeI y'GGCC_r

Cuts at:      0     251     311     345 
   Size:        251      60      34

  Fragments arranged by size: 

                 251      60      34
 
EcoICRI GAG'CTC

Cuts at:      0      66     345 
   Size:         66     279
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     141     345 
   Size:        141     204
 
FspI TGC'GCA

Cuts at:      0     265     345 
   Size:        265      80
 
HaeII r_GCGC'y

Cuts at:      0     261     345 
   Size:        261      84
 
MslI CAynn'nnrTG

Cuts at:      0      25     115     345 
   Size:         25      90     230

  Fragments arranged by size: 

                 230      90      25
 
MspA1I CmG'CkG

Cuts at:      0      78     345 
   Size:         78     267
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      78     141     345 
   Size:         78      63     204

  Fragments arranged by size: 

                 204      78      63
 
PfoI T'CCnGG_A

Cuts at:      0     128     345 
   Size:        128     217
 
PmlI CAC'GTG

Cuts at:      0     123     345 
   Size:        123     222
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     208     240     345 
   Size:        208      32     105

  Fragments arranged by size: 

                 208     105      32
 
PstI C_TGCA'G

Cuts at:      0     181     345 
   Size:        181     164
 
PvuII CAG'CTG

Cuts at:      0      78     345 
   Size:         78     267
 
SacI G_AGCT'C

Cuts at:      0      68     345 
   Size:         68     277
 
SfcI C'TryA_G

Cuts at:      0     177     225     345 
   Size:        177      48     120

  Fragments arranged by size: 

                 177     120      48
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      85     345 
   Size:         85     260
 
TatI w'GTAC_w

Cuts at:      0     107     345 
   Size:        107     238
 
Tth111I GACn'n_nGTC

Cuts at:      0     280     345 
   Size:        280      65
 

 Enzymes that do cut:

     AccI     AleI    AlwNI    ApaLI    BanII Bme1580I     BmrI     BpmI
    BsaAI    BsaBI    BseYI     BsgI  BsiHKAI Bsp1286I    BsrBI    BsrFI
   BssHII    BtgZI     EaeI  EcoICRI  Eco57MI     FspI    HaeII     MslI
   MspA1I    PflMI     PfoI     PmlI     PsrI     PstI    PvuII     SacI
     SfcI    TaqII     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI
    BsaXI    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrDI    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI    FspAI    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PvuI    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TstI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI