(Linear) (Six-base) MAPSORT of: KIBB8449.seq  Check: 7480  from: 1  to: 405



KIBB8449.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

AleI CACnn'nnGTG

Cuts at:      0     175     405 
   Size:        175     230
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     279     405 
   Size:        279     126
 
ApoI r'AATT_y

Cuts at:      0     316     405 
   Size:        316      89
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     255     289     405 
   Size:        255      34     116

  Fragments arranged by size: 

                 255     116      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     304     405 
   Size:        304     101
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      62     364     405 
   Size:         62     302      41

  Fragments arranged by size: 

                 302      62      41
 
BsaHI Gr'CG_yC

Cuts at:      0     164     405 
   Size:        164     241
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     405 
   Size:        115     290
 
BtgI C'CryG_G

Cuts at:      0     156     405 
   Size:        156     249
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      62     364     405 
   Size:         62     302      41

  Fragments arranged by size: 

                 302      62      41
 
EcoO109I rG'GnC_Cy

Cuts at:      0     200     405 
   Size:        200     205
 
EcoRI G'AATT_C

Cuts at:      0     316     405 
   Size:        316      89
 
MslI CAynn'nnrTG

Cuts at:      0     175     405 
   Size:        175     230
 
MspA1I CmG'CkG

Cuts at:      0     219     367     405 
   Size:        219     148      38

  Fragments arranged by size: 

                 219     148      38
 
PvuII CAG'CTG

Cuts at:      0     367     405 
   Size:        367      38
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     226     405 
   Size:        226     179
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     357     405 
   Size:        357      48
 

 Enzymes that do cut:

     AleI     AlfI     ApoI     BcgI    BciVI     BpmI    BsaHI     BsgI
     BtgI  Eco57MI EcoO109I    EcoRI     MslI   MspA1I    PvuII    TaqII
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI