Restriction map for
pF1KB8444
(Linear) (Six-base) MAPSORT of: KIBB8444.seq Check: 8765 from: 1 to: 909
KIBB8444.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 136 909
Size: 136 773
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 488 909
Size: 488 421
AlwNI CAG_nnn'CTG
Cuts at: 0 607 909
Size: 607 302
ApoI r'AATT_y
Cuts at: 0 229 579 909
Size: 229 350 330
Fragments arranged by size:
350 330 229
BamHI G'GATC_C
Cuts at: 0 596 909
Size: 596 313
BbsI GAAGACnn'nnnn_
Cuts at: 0 425 909
Size: 425 484
BciVI GTATCCnnnnn_n'
Cuts at: 0 323 909
Size: 323 586
BfrBI ATG'CAT
Cuts at: 0 616 909
Size: 616 293
Bme1580I G_kGCm'C
Cuts at: 0 489 909
Size: 489 420
BmgBI CAC'GTC
Cuts at: 0 870 909
Size: 870 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 274 909
Size: 67 207 635
Fragments arranged by size:
635 207 67
BsaI GGTCTCn'nnnn_
Cuts at: 0 177 909
Size: 177 732
BsaWI w'CCGG_w
Cuts at: 0 658 909
Size: 658 251
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 624 654 909
Size: 624 30 255
Fragments arranged by size:
624 255 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 12 632 909
Size: 12 620 277
Fragments arranged by size:
620 277 12
Bsp1286I G_dGCh'C
Cuts at: 0 489 909
Size: 489 420
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 136 359 431 615 657 909
Size: 136 223 72 184 42 252
Fragments arranged by size:
252 223 184 136 72 42
BsrBI CCG'CTC
Cuts at: 0 32 909
Size: 32 877
BsrFI r'CCGG_y
Cuts at: 0 51 243 909
Size: 51 192 666
Fragments arranged by size:
666 192 51
BstYI r'GATC_y
Cuts at: 0 266 331 443 547 596 909
Size: 266 65 112 104 49 313
Fragments arranged by size:
313 266 112 104 65 49
BtgI C'CryG_G
Cuts at: 0 96 909
Size: 96 813
DraI TTT'AAA
Cuts at: 0 432 909
Size: 432 477
EaeI y'GGCC_r
Cuts at: 0 501 816 909
Size: 501 315 93
Fragments arranged by size:
501 315 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 24 909
Size: 24 885
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 274 488 909
Size: 67 207 214 421
Fragments arranged by size:
421 214 207 67
EcoNI CCTnn'n_nnAGG
Cuts at: 0 9 909
Size: 9 900
EcoRI G'AATT_C
Cuts at: 0 579 909
Size: 579 330
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 780 909
Size: 780 129
HaeII r_GCGC'y
Cuts at: 0 96 909
Size: 96 813
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 624 656 909
Size: 624 32 253
Fragments arranged by size:
624 253 32
HindIII A'AGCT_T
Cuts at: 0 538 909
Size: 538 371
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 608 909
Size: 608 301
MscI TGG'CCA
Cuts at: 0 818 909
Size: 818 91
MslI CAynn'nnrTG
Cuts at: 0 802 909
Size: 802 107
NaeI GCC'GGC
Cuts at: 0 245 909
Size: 245 664
NgoMIV G'CCGG_C
Cuts at: 0 243 909
Size: 243 666
NsiI A_TGCA'T
Cuts at: 0 618 909
Size: 618 291
PasI CC'CwG_GG
Cuts at: 0 133 909
Size: 133 776
PflMI CCAn_nnn'nTGG
Cuts at: 0 276 909
Size: 276 633
PstI C_TGCA'G
Cuts at: 0 654 909
Size: 654 255
RsrII CG'GwC_CG
Cuts at: 0 27 909
Size: 27 882
SfcI C'TryA_G
Cuts at: 0 356 650 909
Size: 356 294 259
Fragments arranged by size:
356 294 259
StyI C'CwwG_G
Cuts at: 0 764 909
Size: 764 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 360 909
Size: 360 549
Enzymes that do cut:
AarI AcuI AlwNI ApoI BamHI BbsI BciVI BfrBI
Bme1580I BmgBI BpmI BsaI BsaWI BsaXI BseRI Bsp1286I
BspMI BsrBI BsrFI BstYI BtgI DraI EaeI EciI
Eco57MI EcoNI EcoRI FalI HaeII Hin4I HindIII MmeI
MscI MslI NaeI NgoMIV NsiI PasI PflMI PstI
RsrII SfcI StyI TaqII
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BanII BbeI BbvCI
BcgI BclI BglI BglII BlpI BmrI BmtI BplI
Bpu10I BpuEI BsaAI BsaBI BsaHI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraIII DrdI EagI EarI
EcoICRI EcoO109I EcoRV FseI FspI FspAI HincII HpaI
KasI KpnI MfeI MluI MspA1I NarI NcoI NdeI
NheI NotI NruI NspI PacI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI