Restriction map for
pF1KB8441
(Linear) (Six-base) MAPSORT of: KIBB8441.seq Check: 453 from: 1 to: 480
KIBB8441.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 476 480
Size: 476 4
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 125 480
Size: 26 99 355
Fragments arranged by size:
355 99 26
AflII C'TTAA_G
Cuts at: 0 340 480
Size: 340 140
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 367 399 480
Size: 367 32 81
Fragments arranged by size:
367 81 32
AlwNI CAG_nnn'CTG
Cuts at: 0 63 408 480
Size: 63 345 72
Fragments arranged by size:
345 72 63
ApaI G_GGCC'C
Cuts at: 0 222 480
Size: 222 258
AvaI C'yCGr_G
Cuts at: 0 379 480
Size: 379 101
BanII G_rGCy'C
Cuts at: 0 222 480
Size: 222 258
BfrBI ATG'CAT
Cuts at: 0 149 480
Size: 149 331
BlpI GC'TnA_GC
Cuts at: 0 451 480
Size: 451 29
Bme1580I G_kGCm'C
Cuts at: 0 222 480
Size: 222 258
BmgBI CAC'GTC
Cuts at: 0 207 480
Size: 207 273
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 384 480
Size: 6 378 96
Fragments arranged by size:
378 96 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 480
Size: 460 20
BsaI GGTCTCn'nnnn_
Cuts at: 0 34 480
Size: 34 446
BseYI C'CCAG_C
Cuts at: 0 233 480
Size: 233 247
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 480
Size: 448 32
BsmI GAATG_Cn'
Cuts at: 0 151 480
Size: 151 329
Bsp1286I G_dGCh'C
Cuts at: 0 222 480
Size: 222 258
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 476 480
Size: 476 4
BstAPI GCAn_nnn'nTGC
Cuts at: 0 471 480
Size: 471 9
BstYI r'GATC_y
Cuts at: 0 367 480
Size: 367 113
Bsu36I CC'TnA_GG
Cuts at: 0 214 480
Size: 214 266
BtgI C'CryG_G
Cuts at: 0 77 480
Size: 77 403
BtsI GCAGTG_nn'
Cuts at: 0 165 480
Size: 165 315
EaeI y'GGCC_r
Cuts at: 0 248 480
Size: 248 232
EarI CTCTTCn'nnn_
Cuts at: 0 101 480
Size: 101 379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 26 125 384 480
Size: 6 20 99 259 96
Fragments arranged by size:
259 99 96 20 6
EcoO109I rG'GnC_Cy
Cuts at: 0 111 218 219 383 459 480
Size: 111 107 1 164 76 21
Fragments arranged by size:
164 111 107 76 21 1
HaeII r_GCGC'y
Cuts at: 0 418 436 480
Size: 418 18 44
Fragments arranged by size:
418 44 18
MscI TGG'CCA
Cuts at: 0 250 480
Size: 250 230
MslI CAynn'nnrTG
Cuts at: 0 34 480
Size: 34 446
MspA1I CmG'CkG
Cuts at: 0 447 455 480
Size: 447 8 25
Fragments arranged by size:
447 25 8
NcoI C'CATG_G
Cuts at: 0 77 480
Size: 77 403
NsiI A_TGCA'T
Cuts at: 0 151 480
Size: 151 329
PfoI T'CCnGG_A
Cuts at: 0 62 480
Size: 62 418
PpuMI rG'GwC_Cy
Cuts at: 0 111 383 480
Size: 111 272 97
Fragments arranged by size:
272 111 97
PspOMI G'GGCC_C
Cuts at: 0 218 480
Size: 218 262
SmaI CCC'GGG
Cuts at: 0 381 480
Size: 381 99
SmlI C'TyrA_G
Cuts at: 0 340 475 480
Size: 340 135 5
Fragments arranged by size:
340 135 5
StyI C'CwwG_G
Cuts at: 0 77 480
Size: 77 403
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 378 480
Size: 378 102
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 258 480
Size: 258 222
XmaI C'CCGG_G
Cuts at: 0 379 480
Size: 379 101
Enzymes that do cut:
AarI AcuI AflII AloI AlwNI ApaI AvaI BanII
BfrBI BlpI Bme1580I BmgBI BpmI BpuEI BsaI BseYI
BsgI BsmI Bsp1286I BspMI BstAPI BstYI Bsu36I BtgI
BtsI EaeI EarI Eco57MI EcoO109I HaeII MscI MslI
MspA1I NcoI NsiI PfoI PpuMI PspOMI SmaI SmlI
StyI TaqII XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BmrI BmtI BplI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BsiEI BsiHKAI BsiWI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI