Restriction map for
pF1KB8427
(Linear) (Six-base) MAPSORT of: KIBB8427.seq Check: 3452 from: 1 to: 2715
KIBB8427.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AccI GT'mk_AC
Cuts at: 0 254 2384 2715
Size: 254 2130 331
Fragments arranged by size:
2130 331 254
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 482 1174 1495 1795 2098 2183 2188
Size: 262 220 692 321 300 303 85 5
Cuts at: 2188 2602 2715
Size: 414 113
Fragments arranged by size:
692 414 321 303 300 262 220 113
85 5
AflII C'TTAA_G
Cuts at: 0 219 784 2715
Size: 219 565 1931
Fragments arranged by size:
1931 565 219
AflIII A'CryG_T
Cuts at: 0 2251 2715
Size: 2251 464
AhdI GACnn_n'nnGTC
Cuts at: 0 41 2715
Size: 41 2674
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2626 2658 2715
Size: 2626 32 57
Fragments arranged by size:
2626 57 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1405 2698 2715
Size: 1405 1293 17
Fragments arranged by size:
1405 1293 17
ApoI r'AATT_y
Cuts at: 0 395 2406 2715
Size: 395 2011 309
Fragments arranged by size:
2011 395 309
AseI AT'TA_AT
Cuts at: 0 427 1798 2338 2715
Size: 427 1371 540 377
Fragments arranged by size:
1371 540 427 377
BamHI G'GATC_C
Cuts at: 0 873 2715
Size: 873 1842
BanI G'GyrC_C
Cuts at: 0 409 2715
Size: 409 2306
BbsI GAAGACnn'nnnn_
Cuts at: 0 197 268 832 850 2715
Size: 197 71 564 18 1865
Fragments arranged by size:
1865 564 197 71 18
BclI T'GATC_A
Cuts at: 0 1182 2715
Size: 1182 1533
BfrBI ATG'CAT
Cuts at: 0 1302 2715
Size: 1302 1413
BglII A'GATC_T
Cuts at: 0 1923 2715
Size: 1923 792
BlpI GC'TnA_GC
Cuts at: 0 1976 2715
Size: 1976 739
BmtI G_CTAG'C
Cuts at: 0 2404 2715
Size: 2404 311
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 1982 2290 2715
Size: 186 1796 308 425
Fragments arranged by size:
1796 425 308 186
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2626 2656 2715
Size: 2626 30 59
Fragments arranged by size:
2626 59 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1745 2715
Size: 1745 970
BsiHKAI G_wGCw'C
Cuts at: 0 827 2715
Size: 827 1888
BsmI GAATG_Cn'
Cuts at: 0 166 359 2715
Size: 166 193 2356
Fragments arranged by size:
2356 193 166
Bsp1286I G_dGCh'C
Cuts at: 0 827 2715
Size: 827 1888
BsrDI GCAATG_nn'
Cuts at: 0 2663 2715
Size: 2663 52
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1660 2715
Size: 1660 1055
BstYI r'GATC_y
Cuts at: 0 777 873 1923 2715
Size: 777 96 1050 792
Fragments arranged by size:
1050 792 777 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1966 2715
Size: 1966 749
BtsI GCAGTG_nn'
Cuts at: 0 8 361 1609 2715
Size: 8 353 1248 1106
Fragments arranged by size:
1248 1106 353 8
EarI CTCTTCn'nnn_
Cuts at: 0 650 891 2054 2588 2715
Size: 650 241 1163 534 127
Fragments arranged by size:
1163 650 534 241 127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 482 1174 1495 1795 2098 2183 2188
Size: 262 220 692 321 300 303 85 5
Cuts at: 2188 2602 2715
Size: 414 113
Fragments arranged by size:
692 414 321 303 300 262 220 113
85 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 485 1954 2715
Size: 485 1469 761
Fragments arranged by size:
1469 761 485
EcoO109I rG'GnC_Cy
Cuts at: 0 1515 2715
Size: 1515 1200
EcoRV GAT'ATC
Cuts at: 0 2490 2715
Size: 2490 225
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1067 2715
Size: 1067 1648
HaeII r_GCGC'y
Cuts at: 0 986 2715
Size: 986 1729
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 59 104 136 2587 2619 2715
Size: 27 32 45 32 2451 32 96
Fragments arranged by size:
2451 96 45 32 32 32 27
HincII GTy'rAC
Cuts at: 0 380 765 1271 2715
Size: 380 385 506 1444
Fragments arranged by size:
1444 506 385 380
HindIII A'AGCT_T
Cuts at: 0 1494 2715
Size: 1494 1221
HpaI GTT'AAC
Cuts at: 0 380 1271 2715
Size: 380 891 1444
Fragments arranged by size:
1444 891 380
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 1756 2715
Size: 304 1452 959
Fragments arranged by size:
1452 959 304
MslI CAynn'nnrTG
Cuts at: 0 952 2715
Size: 952 1763
MspA1I CmG'CkG
Cuts at: 0 933 2715
Size: 933 1782
NdeI CA'TA_TG
Cuts at: 0 941 1362 2715
Size: 941 421 1353
Fragments arranged by size:
1353 941 421
NheI G'CTAG_C
Cuts at: 0 2400 2715
Size: 2400 315
NsiI A_TGCA'T
Cuts at: 0 1304 2715
Size: 1304 1411
NspI r_CATG'y
Cuts at: 0 2255 2715
Size: 2255 460
PciI A'CATG_T
Cuts at: 0 2251 2715
Size: 2251 464
PflMI CCAn_nnn'nTGG
Cuts at: 0 1053 2715
Size: 1053 1662
PfoI T'CCnGG_A
Cuts at: 0 1776 2715
Size: 1776 939
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2626 2658 2715
Size: 2626 32 57
Fragments arranged by size:
2626 57 32
PpuMI rG'GwC_Cy
Cuts at: 0 1515 2715
Size: 1515 1200
PshAI GACnn'nnGTC
Cuts at: 0 1324 2715
Size: 1324 1391
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2159 2191 2715
Size: 2159 32 524
Fragments arranged by size:
2159 524 32
PvuII CAG'CTG
Cuts at: 0 933 2715
Size: 933 1782
SapI GCTCTTCn'nnn_
Cuts at: 0 2054 2715
Size: 2054 661
ScaI AGT'ACT
Cuts at: 0 1634 2220 2715
Size: 1634 586 495
Fragments arranged by size:
1634 586 495
SfcI C'TryA_G
Cuts at: 0 370 1665 2715
Size: 370 1295 1050
Fragments arranged by size:
1295 1050 370
SmlI C'TyrA_G
Cuts at: 0 165 219 784 1997 2305 2715
Size: 165 54 565 1213 308 410
Fragments arranged by size:
1213 565 410 308 165 54
SspI AAT'ATT
Cuts at: 0 632 713 720 1106 1592 1788 2715
Size: 632 81 7 386 486 196 927
Fragments arranged by size:
927 632 486 386 196 81 7
StyI C'CwwG_G
Cuts at: 0 1250 2606 2715
Size: 1250 1356 109
Fragments arranged by size:
1356 1250 109
TatI w'GTAC_w
Cuts at: 0 47 1572 1632 2218 2367 2427 2715
Size: 47 1525 60 586 149 60 288
Fragments arranged by size:
1525 586 288 149 60 60 47
XbaI T'CTAG_A
Cuts at: 0 1209 2715
Size: 1209 1506
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2671 2715
Size: 2671 44
XmnI GAAnn'nnTTC
Cuts at: 0 756 905 2201 2715
Size: 756 149 1296 514
Fragments arranged by size:
1296 756 514 149
Enzymes that do cut:
AccI AcuI AflII AflIII AhdI AloI AlwNI ApoI
AseI BamHI BanI BbsI BclI BfrBI BglII BlpI
BmtI BpuEI BsaXI BseRI BsiHKAI BsmI Bsp1286I BsrDI
BstAPI BstYI BtgZI BtsI EarI Eco57MI EcoNI EcoO109I
EcoRV FalI HaeII Hin4I HincII HindIII HpaI MmeI
MslI MspA1I NdeI NheI NsiI NspI PciI PflMI
PfoI PpiI PpuMI PshAI PsrI PvuII SapI ScaI
SfcI SmlI SspI StyI TatI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AleI AlfI
ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BanII
BbeI BbvCI BcgI BciVI BglI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII DrdI
EaeI EagI EciI EcoICRI EcoRI FseI FspI FspAI
KasI KpnI MfeI MluI MscI NaeI NarI NcoI
NgoMIV NotI NruI PacI PasI PmeI PmlI PsiI
PspOMI PspXI PstI PvuI RsrII SacI SacII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI
Tth111I XhoI XmaI ZraI