Restriction map for
pF1KB8417
(Linear) (Six-base) MAPSORT of: KIBB8417.seq Check: 2455 from: 1 to: 2355
KIBB8417.orf
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 723 1082 1583 2355
Size: 723 359 501 772
Fragments arranged by size:
772 723 501 359
AflII C'TTAA_G
Cuts at: 0 1867 2355
Size: 1867 488
AflIII A'CryG_T
Cuts at: 0 246 1427 2355
Size: 246 1181 928
Fragments arranged by size:
1181 928 246
AlwNI CAG_nnn'CTG
Cuts at: 0 1198 2355
Size: 1198 1157
ApoI r'AATT_y
Cuts at: 0 792 2355
Size: 792 1563
AseI AT'TA_AT
Cuts at: 0 1292 2355
Size: 1292 1063
BanI G'GyrC_C
Cuts at: 0 3 2355
Size: 3 2352
BanII G_rGCy'C
Cuts at: 0 131 1791 2355
Size: 131 1660 564
Fragments arranged by size:
1660 564 131
BbeI G_GCGC'C
Cuts at: 0 7 2355
Size: 7 2348
BbsI GAAGACnn'nnnn_
Cuts at: 0 729 856 1229 2355
Size: 729 127 373 1126
Fragments arranged by size:
1126 729 373 127
BbvCI CC'TCA_GC
Cuts at: 0 1883 2355
Size: 1883 472
BciVI GTATCCnnnnn_n'
Cuts at: 0 843 1021 1486 2355
Size: 843 178 465 869
Fragments arranged by size:
869 843 465 178
BclI T'GATC_A
Cuts at: 0 159 2355
Size: 159 2196
BglI GCCn_nnn'nGGC
Cuts at: 0 519 2355
Size: 519 1836
BglII A'GATC_T
Cuts at: 0 744 1976 2355
Size: 744 1232 379
Fragments arranged by size:
1232 744 379
Bme1580I G_kGCm'C
Cuts at: 0 1956 2355
Size: 1956 399
BmrI ACTGGGnnnn_n'
Cuts at: 0 223 1537 2355
Size: 223 1314 818
Fragments arranged by size:
1314 818 223
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 69 2355
Size: 69 2286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 987 2355
Size: 987 1368
Bpu10I CC'TnA_GC
Cuts at: 0 220 1883 2355
Size: 220 1663 472
Fragments arranged by size:
1663 472 220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 1544 2355
Size: 100 1444 811
Fragments arranged by size:
1444 811 100
BsaBI GATnn'nnATC
Cuts at: 0 272 2355
Size: 272 2083
BsaHI Gr'CG_yC
Cuts at: 0 4 1121 2355
Size: 4 1117 1234
Fragments arranged by size:
1234 1117 4
BsaWI w'CCGG_w
Cuts at: 0 377 1814 2355
Size: 377 1437 541
Fragments arranged by size:
1437 541 377
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2024 2054 2355
Size: 2024 30 301
Fragments arranged by size:
2024 301 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 572 2355
Size: 572 1783
BseYI C'CCAG_C
Cuts at: 0 1955 2355
Size: 1955 400
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1259 2355
Size: 1259 1096
BsmI GAATG_Cn'
Cuts at: 0 1249 2355
Size: 1249 1106
Bsp1286I G_dGCh'C
Cuts at: 0 131 1791 1956 2355
Size: 131 1660 165 399
Fragments arranged by size:
1660 399 165 131
BsrDI GCAATG_nn'
Cuts at: 0 546 2355
Size: 546 1809
BsrGI T'GTAC_A
Cuts at: 0 1968 2355
Size: 1968 387
BssSI C'ACGA_G
Cuts at: 0 1972 2355
Size: 1972 383
BstAPI GCAn_nnn'nTGC
Cuts at: 0 90 1594 2355
Size: 90 1504 761
Fragments arranged by size:
1504 761 90
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1504 2355
Size: 1504 851
BstYI r'GATC_y
Cuts at: 0 744 1976 2355
Size: 744 1232 379
Fragments arranged by size:
1232 744 379
BtgI C'CryG_G
Cuts at: 0 702 2355
Size: 702 1653
DraI TTT'AAA
Cuts at: 0 2186 2333 2355
Size: 2186 147 22
Fragments arranged by size:
2186 147 22
EaeI y'GGCC_r
Cuts at: 0 59 2355
Size: 59 2296
EarI CTCTTCn'nnn_
Cuts at: 0 136 911 2355
Size: 136 775 1444
Fragments arranged by size:
1444 775 136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 723 987 1082 1583 2355
Size: 723 264 95 501 772
Fragments arranged by size:
772 723 501 264 95
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1022 2355
Size: 1022 1333
EcoO109I rG'GnC_Cy
Cuts at: 0 1015 1879 2355
Size: 1015 864 476
Fragments arranged by size:
1015 864 476
EcoRI G'AATT_C
Cuts at: 0 792 2355
Size: 792 1563
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 426 1087 2355
Size: 426 661 1268
Fragments arranged by size:
1268 661 426
HaeII r_GCGC'y
Cuts at: 0 7 2355
Size: 7 2348
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 936 968 985 1017 2355
Size: 936 32 17 32 1338
Fragments arranged by size:
1338 936 32 32 17
HincII GTy'rAC
Cuts at: 0 2030 2355
Size: 2030 325
HpaI GTT'AAC
Cuts at: 0 2030 2355
Size: 2030 325
KasI G'GCGC_C
Cuts at: 0 3 2355
Size: 3 2352
MfeI C'AATT_G
Cuts at: 0 797 1998 2355
Size: 797 1201 357
Fragments arranged by size:
1201 797 357
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1575 2195 2355
Size: 1575 620 160
Fragments arranged by size:
1575 620 160
MscI TGG'CCA
Cuts at: 0 61 2355
Size: 61 2294
MspA1I CmG'CkG
Cuts at: 0 2256 2293 2355
Size: 2256 37 62
Fragments arranged by size:
2256 62 37
NarI GG'CG_CC
Cuts at: 0 4 2355
Size: 4 2351
NdeI CA'TA_TG
Cuts at: 0 156 2355
Size: 156 2199
NspI r_CATG'y
Cuts at: 0 250 757 1431 2355
Size: 250 507 674 924
Fragments arranged by size:
924 674 507 250
PciI A'CATG_T
Cuts at: 0 246 1427 2355
Size: 246 1181 928
Fragments arranged by size:
1181 928 246
PpuMI rG'GwC_Cy
Cuts at: 0 1015 1879 2355
Size: 1015 864 476
Fragments arranged by size:
1015 864 476
PsiI TTA'TAA
Cuts at: 0 1097 1660 1733 2264 2355
Size: 1097 563 73 531 91
Fragments arranged by size:
1097 563 531 91 73
PvuII CAG'CTG
Cuts at: 0 2293 2355
Size: 2293 62
SapI GCTCTTCn'nnn_
Cuts at: 0 136 2355
Size: 136 2219
ScaI AGT'ACT
Cuts at: 0 89 2355
Size: 89 2266
SfcI C'TryA_G
Cuts at: 0 23 1391 1565 2355
Size: 23 1368 174 790
Fragments arranged by size:
1368 790 174 23
SfoI GGC'GCC
Cuts at: 0 5 2355
Size: 5 2350
SmlI C'TyrA_G
Cuts at: 0 115 1559 1867 2355
Size: 115 1444 308 488
Fragments arranged by size:
1444 488 308 115
SpeI A'CTAG_T
Cuts at: 0 1138 2169 2355
Size: 1138 1031 186
Fragments arranged by size:
1138 1031 186
SphI G_CATG'C
Cuts at: 0 757 2355
Size: 757 1598
SspI AAT'ATT
Cuts at: 0 2099 2355
Size: 2099 256
StyI C'CwwG_G
Cuts at: 0 123 319 2058 2355
Size: 123 196 1739 297
Fragments arranged by size:
1739 297 196 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 588 1382 2287 2355
Size: 588 794 905 68
Fragments arranged by size:
905 794 588 68
TatI w'GTAC_w
Cuts at: 0 87 589 639 1434 1827 1968 2355
Size: 87 502 50 795 393 141 387
Fragments arranged by size:
795 502 393 387 141 87 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2024 2056 2355
Size: 2024 32 299
Fragments arranged by size:
2024 299 32
XmnI GAAnn'nnTTC
Cuts at: 0 905 1067 2355
Size: 905 162 1288
Fragments arranged by size:
1288 905 162
Enzymes that do cut:
AcuI AflII AflIII AlwNI ApoI AseI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsmI Bsp1286I BsrDI BsrGI
BssSI BstAPI BstXI BstYI BtgI DraI EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
HpaI KasI MfeI MmeI MscI MspA1I NarI NdeI
NspI PciI PpuMI PsiI PvuII SapI ScaI SfcI
SfoI SmlI SpeI SphI SspI StyI TaqII TatI
TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BcgI BfrBI BlpI BmgBI BmtI
BsaI BsaAI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI
BspMI BsrBI BsrFI BssHII BstBI BstEII BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraIII DrdI EagI EciI
EcoICRI EcoRV FseI FspI FspAI HindIII KpnI MluI
MslI NaeI NcoI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PmlI PpiI PshAI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SbfI SexAI SfiI SgfI SgrAI SmaI
SnaBI SrfI StuI SwaI Tth111I XbaI XcmI XhoI
XmaI ZraI