Restriction map for
pF1KB8365
(Linear) (Six-base) MAPSORT of: KIBB8365.seq Check: 4936 from: 1 to: 1416
KIBB8365.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AfeI AGC'GCT
Cuts at: 0 637 793 1416
Size: 637 156 623
Fragments arranged by size:
637 623 156
AflIII A'CryG_T
Cuts at: 0 194 755 1416
Size: 194 561 661
Fragments arranged by size:
661 561 194
AlwNI CAG_nnn'CTG
Cuts at: 0 41 1002 1269 1416
Size: 41 961 267 147
Fragments arranged by size:
961 267 147 41
ApaI G_GGCC'C
Cuts at: 0 385 983 1091 1416
Size: 385 598 108 325
Fragments arranged by size:
598 385 325 108
ApaLI G'TGCA_C
Cuts at: 0 937 1416
Size: 937 479
AscI GG'CGCG_CC
Cuts at: 0 965 1416
Size: 965 451
AvaI C'yCGr_G
Cuts at: 0 210 327 976 1090 1416
Size: 210 117 649 114 326
Fragments arranged by size:
649 326 210 117 114
AvrII C'CTAG_G
Cuts at: 0 216 1416
Size: 216 1200
BanI G'GyrC_C
Cuts at: 0 271 340 555 856 895 1033 1137 1416
Size: 271 69 215 301 39 138 104 279
Fragments arranged by size:
301 279 271 215 138 104 69 39
BanII G_rGCy'C
Cuts at: 0 118 265 328 385 418 724 983 1091
Size: 118 147 63 57 33 306 259 108
Cuts at: 1091 1345 1355 1416
Size: 254 10 61
Fragments arranged by size:
306 259 254 147 118 108 63 61
57 33 10
BbeI G_GCGC'C
Cuts at: 0 275 344 559 860 899 1037 1416
Size: 275 69 215 301 39 138 379
Fragments arranged by size:
379 301 275 215 138 69 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 952 1054 1416
Size: 952 102 362
Fragments arranged by size:
952 362 102
BbvCI CC'TCA_GC
Cuts at: 0 1356 1416
Size: 1356 60
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 741 775 1416
Size: 741 34 641
Fragments arranged by size:
741 641 34
BglI GCCn_nnn'nGGC
Cuts at: 0 332 458 1416
Size: 332 126 958
Fragments arranged by size:
958 332 126
BglII A'GATC_T
Cuts at: 0 584 1416
Size: 584 832
BlpI GC'TnA_GC
Cuts at: 0 322 577 1416
Size: 322 255 839
Fragments arranged by size:
839 322 255
Bme1580I G_kGCm'C
Cuts at: 0 385 941 983 1091 1112 1416
Size: 385 556 42 108 21 304
Fragments arranged by size:
556 385 304 108 42 21
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 93 1416
Size: 93 1323
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1037 1384 1416
Size: 1037 347 32
Fragments arranged by size:
1037 347 32
Bpu10I CC'TnA_GC
Cuts at: 0 1356 1416
Size: 1356 60
BsaHI Gr'CG_yC
Cuts at: 0 184 272 341 473 556 857 896 1034
Size: 184 88 69 132 83 301 39 138
Cuts at: 1034 1416
Size: 382
Fragments arranged by size:
382 301 184 138 132 88 83 69
39
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 730 760 1183 1213 1357 1387 1416
Size: 730 30 423 30 144 30 29
Fragments arranged by size:
730 423 144 30 30 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 69 1050 1212 1416
Size: 69 981 162 204
Fragments arranged by size:
981 204 162 69
BseYI C'CCAG_C
Cuts at: 0 559 907 1151 1416
Size: 559 348 244 265
Fragments arranged by size:
559 348 265 244
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 1141 1416
Size: 721 420 275
Fragments arranged by size:
721 420 275
BsiEI CG_ry'CG
Cuts at: 0 296 395 1416
Size: 296 99 1021
Fragments arranged by size:
1021 296 99
BsiHKAI G_wGCw'C
Cuts at: 0 941 1416
Size: 941 475
BsiWI C'GTAC_G
Cuts at: 0 425 1416
Size: 425 991
Bsp1286I G_dGCh'C
Cuts at: 0 118 265 328 385 418 724 941 983
Size: 118 147 63 57 33 306 217 42
Cuts at: 983 1091 1112 1345 1355 1416
Size: 108 21 233 10 61
Fragments arranged by size:
306 233 217 147 118 108 63 61
57 42 33 21 10
BsrBI CCG'CTC
Cuts at: 0 167 332 839 1416
Size: 167 165 507 577
Fragments arranged by size:
577 507 167 165
BsrFI r'CCGG_y
Cuts at: 0 223 247 295 620 820 949 969 1368
Size: 223 24 48 325 200 129 20 399
Cuts at: 1368 1416
Size: 48
Fragments arranged by size:
399 325 223 200 129 48 48 24
20
BssHII G'CGCG_C
Cuts at: 0 965 1416
Size: 965 451
BssSI C'ACGA_G
Cuts at: 0 1144 1416
Size: 1144 272
BstEII G'GTnAC_C
Cuts at: 0 1329 1416
Size: 1329 87
BstYI r'GATC_y
Cuts at: 0 584 1416
Size: 584 832
BtgI C'CryG_G
Cuts at: 0 308 344 419 539 1416
Size: 308 36 75 120 877
Fragments arranged by size:
877 308 120 75 36
BtsI GCAGTG_nn'
Cuts at: 0 1267 1416
Size: 1267 149
DrdI GACnn_nn'nnGTC
Cuts at: 0 183 682 1416
Size: 183 499 734
Fragments arranged by size:
734 499 183
EaeI y'GGCC_r
Cuts at: 0 293 305 392 542 951 1416
Size: 293 12 87 150 409 465
Fragments arranged by size:
465 409 293 150 87 12
EagI C'GGCC_G
Cuts at: 0 293 392 1416
Size: 293 99 1024
Fragments arranged by size:
1024 293 99
EarI CTCTTCn'nnn_
Cuts at: 0 613 1416
Size: 613 803
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 643 1416
Size: 643 773
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1037 1384 1416
Size: 1037 347 32
Fragments arranged by size:
1037 347 32
EcoO109I rG'GnC_Cy
Cuts at: 0 382 885 1170 1416
Size: 382 503 285 246
Fragments arranged by size:
503 382 285 246
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1263 1416
Size: 1263 153
FseI GG_CCGG'CC
Cuts at: 0 227 1416
Size: 227 1189
FspI TGC'GCA
Cuts at: 0 353 1416
Size: 353 1063
HaeII r_GCGC'y
Cuts at: 0 275 322 344 559 639 795 860 899
Size: 275 47 22 215 80 156 65 39
Cuts at: 899 914 1037 1099 1325 1416
Size: 15 123 62 226 91
Fragments arranged by size:
275 226 215 156 123 91 80 65
62 47 39 22 15
KasI G'GCGC_C
Cuts at: 0 271 340 555 856 895 1033 1416
Size: 271 69 215 301 39 138 383
Fragments arranged by size:
383 301 271 215 138 69 39
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 1416
Size: 461 955
MscI TGG'CCA
Cuts at: 0 544 1416
Size: 544 872
MslI CAynn'nnrTG
Cuts at: 0 139 145 538 932 1416
Size: 139 6 393 394 484
Fragments arranged by size:
484 394 393 139 6
MspA1I CmG'CkG
Cuts at: 0 854 1155 1416
Size: 854 301 261
Fragments arranged by size:
854 301 261
NaeI GCC'GGC
Cuts at: 0 225 249 297 822 971 1370 1416
Size: 225 24 48 525 149 399 46
Fragments arranged by size:
525 399 225 149 48 46 24
NarI GG'CG_CC
Cuts at: 0 272 341 556 857 896 1034 1416
Size: 272 69 215 301 39 138 382
Fragments arranged by size:
382 301 272 215 138 69 39
NcoI C'CATG_G
Cuts at: 0 308 344 419 539 1416
Size: 308 36 75 120 877
Fragments arranged by size:
877 308 120 75 36
NgoMIV G'CCGG_C
Cuts at: 0 223 247 295 820 969 1368 1416
Size: 223 24 48 525 149 399 48
Fragments arranged by size:
525 399 223 149 48 48 24
NotI GC'GGCC_GC
Cuts at: 0 392 1416
Size: 392 1024
NspI r_CATG'y
Cuts at: 0 198 255 759 1416
Size: 198 57 504 657
Fragments arranged by size:
657 504 198 57
PciI A'CATG_T
Cuts at: 0 194 755 1416
Size: 194 561 661
Fragments arranged by size:
661 561 194
PflMI CCAn_nnn'nTGG
Cuts at: 0 41 1002 1416
Size: 41 961 414
Fragments arranged by size:
961 414 41
PpuMI rG'GwC_Cy
Cuts at: 0 885 1170 1416
Size: 885 285 246
Fragments arranged by size:
885 285 246
PspOMI G'GGCC_C
Cuts at: 0 381 979 1087 1416
Size: 381 598 108 329
Fragments arranged by size:
598 381 329 108
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 37 69 757 789 1416
Size: 37 32 688 32 627
Fragments arranged by size:
688 627 37 32 32
PvuII CAG'CTG
Cuts at: 0 1155 1416
Size: 1155 261
SanDI GG'GwC_CC
Cuts at: 0 1170 1416
Size: 1170 246
SfoI GGC'GCC
Cuts at: 0 273 342 557 858 897 1035 1416
Size: 273 69 215 301 39 138 381
Fragments arranged by size:
381 301 273 215 138 69 39
SmaI CCC'GGG
Cuts at: 0 212 978 1092 1416
Size: 212 766 114 324
Fragments arranged by size:
766 324 212 114
SphI G_CATG'C
Cuts at: 0 255 1416
Size: 255 1161
StuI AGG'CCT
Cuts at: 0 73 110 1416
Size: 73 37 1306
Fragments arranged by size:
1306 73 37
StyI C'CwwG_G
Cuts at: 0 216 308 344 419 539 860 1380 1416
Size: 216 92 36 75 120 321 520 36
Fragments arranged by size:
520 321 216 120 92 75 36 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 337 1416
Size: 337 1079
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 649 681 730 762 1183 1215 1416
Size: 649 32 49 32 421 32 201
Fragments arranged by size:
649 421 201 49 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 375 1416
Size: 375 1041
XmaI C'CCGG_G
Cuts at: 0 210 976 1090 1416
Size: 210 766 114 326
Fragments arranged by size:
766 326 210 114
XmnI GAAnn'nnTTC
Cuts at: 0 793 1416
Size: 793 623
Enzymes that do cut:
AfeI AflIII AlwNI ApaI ApaLI AscI AvaI AvrII
BanI BanII BbeI BbsI BbvCI BcgI BglI BglII
BlpI Bme1580I BplI BpmI Bpu10I BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BsrBI BsrFI
BssHII BssSI BstEII BstYI BtgI BtsI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I FalI FseI FspI
HaeII KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PciI PflMI PpuMI PspOMI
PsrI PvuII SanDI SfoI SmaI SphI StuI StyI
TaqII TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AleI AlfI AloI ApoI AseI AsiSI BaeI
BamHI BciVI BclI BfrBI BmgBI BmrI BmtI BpuEI
BsaI BsaAI BsaBI BsaWI BsmI BsmBI BspEI BspHI
BspMI BsrDI BsrGI BstAPI BstBI BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII EcoICRI EcoNI EcoRI
EcoRV FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI NdeI NheI NruI NsiI PacI PasI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI
SspI SwaI TatI Tth111I XbaI XhoI ZraI