Restriction map for
pF1KB8357
(Linear) (Six-base) MAPSORT of: KIBB8357.seq Check: 3622 from: 1 to: 789
KIBB8357.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AatII G_ACGT'C
Cuts at: 0 47 190 789
Size: 47 143 599
Fragments arranged by size:
599 143 47
AccI GT'mk_AC
Cuts at: 0 53 789
Size: 53 736
AhdI GACnn_n'nnGTC
Cuts at: 0 186 789
Size: 186 603
AleI CACnn'nnGTG
Cuts at: 0 238 789
Size: 238 551
ApoI r'AATT_y
Cuts at: 0 400 789
Size: 400 389
AvaI C'yCGr_G
Cuts at: 0 524 789
Size: 524 265
BamHI G'GATC_C
Cuts at: 0 631 789
Size: 631 158
BanII G_rGCy'C
Cuts at: 0 200 789
Size: 200 589
BbvCI CC'TCA_GC
Cuts at: 0 136 789
Size: 136 653
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 252 286 789
Size: 252 34 503
Fragments arranged by size:
503 252 34
BlpI GC'TnA_GC
Cuts at: 0 553 789
Size: 553 236
BmgBI CAC'GTC
Cuts at: 0 51 789
Size: 51 738
BmrI ACTGGGnnnn_n'
Cuts at: 0 653 789
Size: 653 136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 789
Size: 196 593
Bpu10I CC'TnA_GC
Cuts at: 0 136 206 789
Size: 136 70 583
Fragments arranged by size:
583 136 70
BsaBI GATnn'nnATC
Cuts at: 0 280 789
Size: 280 509
BsaHI Gr'CG_yC
Cuts at: 0 44 187 789
Size: 44 143 602
Fragments arranged by size:
602 143 44
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 28 789
Size: 28 761
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 789
Size: 19 770
BseYI C'CCAG_C
Cuts at: 0 514 761 776 789
Size: 514 247 15 13
Fragments arranged by size:
514 247 15 13
BsiHKAI G_wGCw'C
Cuts at: 0 29 200 675 789
Size: 29 171 475 114
Fragments arranged by size:
475 171 114 29
Bsp1286I G_dGCh'C
Cuts at: 0 29 200 675 789
Size: 29 171 475 114
Fragments arranged by size:
475 171 114 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 254 789
Size: 254 535
BsrBI CCG'CTC
Cuts at: 0 11 789
Size: 11 778
BsrDI GCAATG_nn'
Cuts at: 0 361 789
Size: 361 428
BsrFI r'CCGG_y
Cuts at: 0 131 789
Size: 131 658
BstYI r'GATC_y
Cuts at: 0 631 789
Size: 631 158
BtgI C'CryG_G
Cuts at: 0 67 81 789
Size: 67 14 708
Fragments arranged by size:
708 67 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 468 789
Size: 468 321
BtsI GCAGTG_nn'
Cuts at: 0 143 400 704 789
Size: 143 257 304 85
Fragments arranged by size:
304 257 143 85
DrdI GACnn_nn'nnGTC
Cuts at: 0 49 789
Size: 49 740
EaeI y'GGCC_r
Cuts at: 0 129 685 789
Size: 129 556 104
Fragments arranged by size:
556 129 104
EcoICRI GAG'CTC
Cuts at: 0 198 789
Size: 198 591
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 789
Size: 196 593
EcoNI CCTnn'n_nnAGG
Cuts at: 0 572 789
Size: 572 217
EcoO109I rG'GnC_Cy
Cuts at: 0 210 433 789
Size: 210 223 356
Fragments arranged by size:
356 223 210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 103 135 172 204 623 655 789
Size: 103 32 37 32 419 32 134
Fragments arranged by size:
419 134 103 37 32 32 32
MscI TGG'CCA
Cuts at: 0 687 789
Size: 687 102
MslI CAynn'nnrTG
Cuts at: 0 238 789
Size: 238 551
MspA1I CmG'CkG
Cuts at: 0 69 108 238 514 789
Size: 69 39 130 276 275
Fragments arranged by size:
276 275 130 69 39
NaeI GCC'GGC
Cuts at: 0 133 789
Size: 133 656
NgoMIV G'CCGG_C
Cuts at: 0 131 789
Size: 131 658
PasI CC'CwG_GG
Cuts at: 0 574 607 789
Size: 574 33 182
Fragments arranged by size:
574 182 33
PvuII CAG'CTG
Cuts at: 0 108 238 514 789
Size: 108 130 276 275
Fragments arranged by size:
276 275 130 108
SacI G_AGCT'C
Cuts at: 0 200 789
Size: 200 589
SacII CC_GC'GG
Cuts at: 0 70 789
Size: 70 719
SfcI C'TryA_G
Cuts at: 0 87 737 789
Size: 87 650 52
Fragments arranged by size:
650 87 52
StyI C'CwwG_G
Cuts at: 0 429 789
Size: 429 360
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 347 372 789
Size: 347 25 417
Fragments arranged by size:
417 347 25
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 773 789
Size: 773 16
ZraI GAC'GTC
Cuts at: 0 45 188 789
Size: 45 143 601
Fragments arranged by size:
601 143 45
Enzymes that do cut:
AatII AccI AhdI AleI ApoI AvaI BamHI BanII
BbvCI BcgI BlpI BmgBI BmrI BpmI Bpu10I BsaBI
BsaHI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI BsrBI
BsrDI BsrFI BstYI BtgI BtgZI BtsI DrdI EaeI
EcoICRI Eco57MI EcoNI EcoO109I Hin4I MscI MslI MspA1I
NaeI NgoMIV PasI PvuII SacI SacII SfcI StyI
TaqII XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BanI BbeI BbsI BciVI BclI BfrBI
BglI BglII Bme1580I BmtI BplI BpuEI BsaI BsaAI
BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraI DraIII EagI EarI EciI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NarI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI TstI Tth111I XbaI XhoI XmaI
XmnI