Restriction map for
pF1KB8327
(Linear) (Six-base) MAPSORT of: KIBB8327.seq Check: 2090 from: 1 to: 762
KIBB8327.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 129 762
Size: 129 633
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 524 762
Size: 524 238
ApaI G_GGCC'C
Cuts at: 0 324 762
Size: 324 438
AvaI C'yCGr_G
Cuts at: 0 134 176 261 762
Size: 134 42 85 501
Fragments arranged by size:
501 134 85 42
BanII G_rGCy'C
Cuts at: 0 63 324 762
Size: 63 261 438
Fragments arranged by size:
438 261 63
BbvCI CC'TCA_GC
Cuts at: 0 649 701 762
Size: 649 52 61
Fragments arranged by size:
649 61 52
BclI T'GATC_A
Cuts at: 0 191 762
Size: 191 571
BglI GCCn_nnn'nGGC
Cuts at: 0 47 762
Size: 47 715
BglII A'GATC_T
Cuts at: 0 542 762
Size: 542 220
BlpI GC'TnA_GC
Cuts at: 0 37 762
Size: 37 725
Bme1580I G_kGCm'C
Cuts at: 0 324 762
Size: 324 438
BmrI ACTGGGnnnn_n'
Cuts at: 0 151 762
Size: 151 611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 476 762
Size: 77 399 286
Fragments arranged by size:
399 286 77
Bpu10I CC'TnA_GC
Cuts at: 0 649 701 762
Size: 649 52 61
Fragments arranged by size:
649 61 52
BsaI GGTCTCn'nnnn_
Cuts at: 0 376 762
Size: 376 386
BsaAI yAC'GTr
Cuts at: 0 110 762
Size: 110 652
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 271 631 634 673 762
Size: 271 360 3 39 89
Fragments arranged by size:
360 271 89 39 3
BseYI C'CCAG_C
Cuts at: 0 117 185 201 762
Size: 117 68 16 561
Fragments arranged by size:
561 117 68 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 762
Size: 84 678
Bsp1286I G_dGCh'C
Cuts at: 0 63 324 762
Size: 63 261 438
Fragments arranged by size:
438 261 63
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 122 762
Size: 122 640
BstYI r'GATC_y
Cuts at: 0 542 762
Size: 542 220
BtgI C'CryG_G
Cuts at: 0 474 591 611 762
Size: 474 117 20 151
Fragments arranged by size:
474 151 117 20
BtsI GCAGTG_nn'
Cuts at: 0 234 755 762
Size: 234 521 7
Fragments arranged by size:
521 234 7
EaeI y'GGCC_r
Cuts at: 0 399 762
Size: 399 363
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 501 762
Size: 19 482 261
Fragments arranged by size:
482 261 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 476 762
Size: 77 399 286
Fragments arranged by size:
399 286 77
EcoO109I rG'GnC_Cy
Cuts at: 0 320 762
Size: 320 442
HaeII r_GCGC'y
Cuts at: 0 175 372 762
Size: 175 197 390
Fragments arranged by size:
390 197 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 50 82 115 147 337 341 369 373
Size: 50 32 33 32 190 4 28 4
Cuts at: 373 762
Size: 389
Fragments arranged by size:
389 190 50 33 32 32 28 4
4
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 359 762
Size: 359 403
MscI TGG'CCA
Cuts at: 0 401 762
Size: 401 361
MspA1I CmG'CkG
Cuts at: 0 36 189 653 762
Size: 36 153 464 109
Fragments arranged by size:
464 153 109 36
NcoI C'CATG_G
Cuts at: 0 591 762
Size: 591 171
NspI r_CATG'y
Cuts at: 0 735 762
Size: 735 27
PflMI CCAn_nnn'nTGG
Cuts at: 0 444 762
Size: 444 318
PfoI T'CCnGG_A
Cuts at: 0 161 555 762
Size: 161 394 207
Fragments arranged by size:
394 207 161
PspOMI G'GGCC_C
Cuts at: 0 320 762
Size: 320 442
PvuII CAG'CTG
Cuts at: 0 36 189 653 762
Size: 36 153 464 109
Fragments arranged by size:
464 153 109 36
SmaI CCC'GGG
Cuts at: 0 136 762
Size: 136 626
StyI C'CwwG_G
Cuts at: 0 591 762
Size: 591 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 109 467 519 762
Size: 109 358 52 243
Fragments arranged by size:
358 243 109 52
TatI w'GTAC_w
Cuts at: 0 12 762
Size: 12 750
Tth111I GACn'n_nGTC
Cuts at: 0 157 762
Size: 157 605
XmaI C'CCGG_G
Cuts at: 0 134 762
Size: 134 628
Enzymes that do cut:
AhdI AlfI ApaI AvaI BanII BbvCI BclI BglI
BglII BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI
BseRI BseYI BsgI Bsp1286I BspMI BstYI BtgI BtsI
EaeI EciI Eco57MI EcoO109I HaeII Hin4I MmeI MscI
MspA1I NcoI NspI PflMI PfoI PspOMI PvuII SmaI
StyI TaqII TatI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BcgI BciVI BfrBI BmgBI BmtI BplI BpuEI BsaBI
BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MslI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI XbaI XcmI XhoI XmnI ZraI